The landscape in telomere related gene prognostic signature for survival and medication treatment effectiveness prediction in hepatocellular carcinoma

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 18, 2024

Telomeres, made of repetitive DNA sequences and shelterin complexes, which were found at the ends chromosomes had been extensively studied in cancer research. However, hepatocellular carcinoma (HCC) was still relatively scarce. In this study, we investigated correlation between telomerase-related genes (TRGs) prognosis immunotherapy HCC patients to enhance clinical outcomes. work, TRGs gathered using TelNet, while information gene expression data for retrieved from Cancer Genome Atlas (TCGA) database. A risk prediction model based on created COX Lasso regression analyses, with ROC curves used assess prognostic efficacy. Univariate multifactorial analyses determine if an independent impact prognosis. Nomograms usability, calibration predictive ability various time points. The Tumor Immune Dysfunction Exclusion (TIDE) score analyze differences immune infiltrating cells groups. study analyzed relationship ratings drug treatment effectiveness CellMiner hub identified its markers examined. cell subpopulations also by single-cell data. 2093 identified, 949 showing significant paracancerous tissues. Seven overexpressed tumor tissues, leading lower survival rates high-risk patients. Risk could independently predict Analysis revealed abundance groups, notable variations subset enrichment subgroups. Higher scores correlated increased sensitivity sorafenib, mitoxantrone, oxaliplatin, gemcitabine, entinostat, decreased vincristine, etc. CDCA8 as a key Protein Interaction Network, high associated poorer overall survival, proliferation metastasis. results analysis suggest that may promote development affecting T lymphocytes. TRG-based patient closely linked environment, offer new possibilities treatment.

Language: Английский

Roles of WDR12 and MRTO4 genes in colorectal cancer

Medicine, Journal Year: 2024, Volume and Issue: 103(52), P. e41048 - e41048

Published: Dec. 27, 2024

Colorectal cancer refers to malignant tumors occurring in the walls of colon or rectum. The roles WD Repeat Domain 12 (WDR12) and mitochondrial ribosome-associated tumor suppressor 4 (MRTO4) genes colorectal remain unclear. dataset GSE113513 configuration file was downloaded from gene expression omnibus database generated GPL15207. Differentially expressed screening, functional enrichment analysis, set Weighted Gene Co-expression Network Analysis, construction analysis protein-protein interaction networks, survival heatmap plotting were conducted. Comparative toxicogenomics performed find diseases most relevant core genes. TargetScan used screen miRNAs regulating A total 3106 differentially identified. According ontology they mainly enriched organic acid metabolic processes, condensed chromosome kinetochore, oxidoreductase activity, cell cycle. In Kyoto encyclopedia genomes primarily concentrated cycle, TGF-β signaling pathway, Jak-STAT PI3K-Akt Ras TNF p53 NF-kB WNT pathway. Analysis with a soft thresholding power 29 modules. network identified 6 (DDX27, NAT10, WDR12, DKC1, MRTO4, NOP56). Survival showed (POSTN, MYH11, LUM, COL6A3, COL4A1) as risk factors. revealed high (WDR12 MRTO4) samples. linked local infiltration, bowel obstruction, abdominal pain, neoplasms. WDR12 MRTO4 are highly cancer, potentially influencing its progression.

Language: Английский