Eluding anemone nematocysts: are clownfish deprived of N-acetylated sugars in their skin mucus? DOI Creative Commons
Sara Heim,

Tony Teav,

Fabio Cortesi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 11, 2024

The clownfish - sea anemone system is a great example of symbiotic mutualism where host 'toxicity' does not impact its symbiont partner, although the underlying protection mechanism remains unclear. regulation nematocyst discharge in cnidarians involves N-acetylated sugars like sialic acid, that bind chemoreceptors on tentacles anemones, leading to release stings. It has been suggested could be deprived acid their skin surface, sparing them from being stung and facilitating with anemones. In this study, we sampled mucus two symbionts, Amphiprion akindynos juvenile damselfish Dascyllus trimaculatus, as well non-symbiotic adult Pomacentrus moluccensis P. pavo. free total content, including conjugated form, three other intermediates pathway were quantified using stable isotope dilution mass spectrometry approach. We found significantly higher levels precursor damselfishes. Concentrations symbionts ranged between 13 uM 16 uM, whereas damselfishes 21 30 uM. presence metabolite precursors, triggers discharge, suggests direct or trigger concentration dependent. This experiment demonstrates are spared by nematocysts because lack sugars, previously thought.

Language: Английский

N-acetylated sugars in clownfish and damselfish skin mucus as messengers involved in chemical recognition by anemone host DOI Creative Commons
Sara Heim,

Tony Teav,

Fabio Cortesi

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 15, 2025

The clownfish - sea anemone system is a great example of symbiotic mutualism where host «toxicity» does not impact its symbiont partner, although the underlying protection mechanism remains unclear. regulation nematocyst discharge in cnidarians involves N-acetylated sugars like sialic acid, that bind chemoreceptors on tentacles anemones, leading to release stings. It has been suggested could be deprived acid their skin surface, sparing them from being stung and facilitating with anemones. In this study, we sampled mucus two symbionts, Amphiprion akindynos juvenile damselfish Dascyllus trimaculatus, as well non-symbiotic adult Pomacentrus moluccensis P. pavo. free total content, including conjugated form, three other intermediates pathway were quantified using stable isotope dilution mass spectrometry approach. We found significantly higher levels precursor damselfishes. Concentrations symbionts ranged between 13 µM 16 µM, whereas damselfishes 21 30 µM. presence metabolite precursors, triggers discharge, suggests direct or trigger concentration dependent. This experiment demonstrates are spared by nematocysts because lack sugars, previously thought, rather biochemical mechanisms involving more complex than just presence/absence these molecules.

Language: Английский

Citations

0
Mechanistic toxicity and growth abnormalities mediated by subacute exposure to environmentally relevant levels of benzophenone-3 in clown anemonefish (Amphiprion ocellaris) DOI

Yankun Zhang,

Yongqiang Qin,

Hanye Ju

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 902, P. 166308 - 166308

Published: Aug. 16, 2023

Language: Английский

Citations

9
The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of Pomacentrids DOI Creative Commons
Emma Gairin, Saori Miura,

Hiroki Takamiyagi

et al.

Gigabyte, Journal Year: 2024, Volume and Issue: 2024

Published: Dec. 31, 2024

The number of high-quality genomes is rapidly increasing across taxa. However, it remains limited for coral reef fish the Pomacentrid family, with most research focused on anemonefish. Here, we present first assembly a genus Chrysiptera. Using PacBio long-read sequencing 94.5× coverage, genome Sapphire Devil, Chrysiptera cyanea, was assembled and annotated. final comprises 896 Mb pairs 91 contigs, BUSCO completeness 97.6%, 28,173 genes. Comparative analyses chromosome-scale assemblies related species identified contig-chromosome correspondences. This will be useful as comparison to study specific adaptations linked symbiotic life closely Furthermore, C. cyanea found in tropical coastal areas Indo-West Pacific could become model environmental monitoring. work expand efforts, highlighting power retrieve high quality genomes.

Language: Английский

Citations

1
Artificial interspecific hybridization of the two distant anemonefish species and phenotypic characterization of the F1 hybrid DOI
Haruka Yoshida, Tomoyuki Kokita

Ichthyological Research, Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

Language: Английский

Citations

0
Eluding anemone nematocysts: are clownfish deprived of N-acetylated sugars in their skin mucus? DOI Creative Commons
Sara Heim,

Tony Teav,

Fabio Cortesi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 11, 2024

The clownfish - sea anemone system is a great example of symbiotic mutualism where host 'toxicity' does not impact its symbiont partner, although the underlying protection mechanism remains unclear. regulation nematocyst discharge in cnidarians involves N-acetylated sugars like sialic acid, that bind chemoreceptors on tentacles anemones, leading to release stings. It has been suggested could be deprived acid their skin surface, sparing them from being stung and facilitating with anemones. In this study, we sampled mucus two symbionts, Amphiprion akindynos juvenile damselfish Dascyllus trimaculatus, as well non-symbiotic adult Pomacentrus moluccensis P. pavo. free total content, including conjugated form, three other intermediates pathway were quantified using stable isotope dilution mass spectrometry approach. We found significantly higher levels precursor damselfishes. Concentrations symbionts ranged between 13 uM 16 uM, whereas damselfishes 21 30 uM. presence metabolite precursors, triggers discharge, suggests direct or trigger concentration dependent. This experiment demonstrates are spared by nematocysts because lack sugars, previously thought.

Language: Английский

Citations

0