Mitochondrial Fusion Protein 2‐Modified Bone Marrow Mesenchymal Stem Cells Improved Hyperglycemia‐Induced Schwann Cell Injury via Regulating Mitochondria‐Associated Endoplasmic Reticulum Membranes

Neurourology and Urodynamics, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

ABSTRACT Objective High glucose damages rat Schwann cells (SCs), which is closely related to the dysfunction of mitochondria‐associated endoplasmic reticulum membranes (MAMs). Therefore, present study aimed investigate protective effects and mechanisms modified bone marrow mesenchymal stem (BMSCs) mitochondrial fusion protein 2 (Mfn2) BMSCs against SCs injury. Methods The Mfn2‐modified were constructed after culturing with neural‐induced differentiation solution. MAP‐2 (microtubule‐associated protein‐2, neuron marker) GFAP (glial fibrillary acidic protein, astrocytes immunofluorescence staining was used observe changes in potential neural‐like BMSCs. (RSC96) cultured under high conditions cocultured Changes functional expression MAMs detected by Western Blot. Transmission electron microscopy (TEM) microscopic morphology MAMs, mitochondria reticulum. Results level Mfn2 significantly increased transfected Mfn2. fluorescence densities upregulated Mfn2‐BMSCs induction neural inducible When RSC96 incubated Mfn2‐modified/non‐modified BMSCs, increased, while PERK, IP3R Drp1 expressions reduced. And showed more significant comparing Mfn2‐non‐modified TEM structural integrity clear structure cristae obvious structurally intact extension Mfn2‐BMSC group. Conclusions transfection promoted cell modulated homeostasis regulating levels proteins.

Language: Английский

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A guide to diabetic bladder dysfunction

Independent Nurse, Journal Year: 2025, Volume and Issue: 2025(5), P. 14 - 22

Published: May 2, 2025

Diabetes affects multiple body systems and can have a major effect on health well being. Here Linda Nazarko explains how type two diabetes bladder function in women

Language: Английский

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Study on OAB Symptoms and Quality of Life in Elderly Patients with Diabetes Mellitus Complicated with Bladder Dysfunction

Advances in Clinical Medicine, Journal Year: 2024, Volume and Issue: 14(08), P. 560 - 569

Published: Jan. 1, 2024

Language: Английский

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