Resolution enhancement with deblurring by pixel reassignment (DPR) DOI Creative Commons
Bingying Zhao, Jérôme Mertz

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 24, 2023

Improving the spatial resolution of a fluorescence microscope has been an ongoing challenge in imaging community. To address this challenge, variety approaches have taken, ranging from instrumentation development to image post-processing. An example latter is deconvolution, where images are numerically deblurred based on knowledge point spread function. However, deconvolution can easily lead noise-amplification artifacts. Deblurring by post-processing also negativities or fail conserve local linearity between sample and image. We describe here simple deblurring algorithm pixel reassignment that inherently avoids such artifacts be applied general modalities fluorophore types. Our helps distinguish nearby fluorophores even when these separated distances smaller than conventional limit, helping facilitate, for example, application single-molecule localization microscopy dense samples. demonstrate versatility performance our under conditions.

Language: Английский

Resolution enhancement with deblurring by pixel reassignment DOI Creative Commons
Bingying Zhao, Jérôme Mertz

Advanced Photonics, Journal Year: 2023, Volume and Issue: 5(06)

Published: Oct. 27, 2023

Improving the spatial resolution of a fluorescence microscope has been an ongoing challenge in imaging community. To address this challenge, variety approaches have taken, ranging from instrumentation development to image postprocessing. An example latter is deconvolution, where images are numerically deblurred based on knowledge point spread function. However, deconvolution can easily lead noise-amplification artifacts. Deblurring by postprocessing also negativities or fail conserve local linearity between sample and image. We describe here simple deblurring algorithm pixel reassignment that inherently avoids such artifacts be applied general modalities fluorophore types. Our helps distinguish nearby fluorophores, even when these separated distances smaller than conventional limit, helping facilitate, for example, application single-molecule localization microscopy dense samples. demonstrate versatility performance our under conditions.

Language: Английский

Citations

33
Real-time targeted illumination in widefield microscopy achieves confocal quality neuronal images DOI Open Access
Yao L. Wang,

Jia Fan,

Samuel Chung

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 10, 2023

Abstract Widefield fluorescence imaging has significant challenges in visualizing neuronal fibers near cell bodies. Specifically, out-of-focus and scattered light from the bright cellbody often obscures nearby dim degrades their signal-to-background ratio. Scanning techniques can solve this problem but are limited by reduced speed increased cost. We greatly reduce stray modulating illumination intensity to different structures. use a digital micromirror device channel of common widefield microscope real-time image processing pattern illumination. With setup, we illuminate bodies with minimal intensity, focus fiber-like structures high reveal weak signals. Thus, minimize background enhance visibility final image. This targeted significantly improves fiber contrast while maintaining fast-imaging low Using setup microscope, demonstrate confocal quality complex neurons live C. elegans zebrafish larva, as well vitro mice brain slice.

Language: Английский

Citations

1
Research on the Method of Depth-Sensing Optical System Based on Multi-Layer Interface Reflection DOI Creative Commons
Yu Chen, Ying Liu, Linhan Li

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(22), P. 7228 - 7228

Published: Nov. 12, 2024

In this paper, a depth-sensing method employing active irradiation of semi-annular beam is proposed for observing the multi-layered reflective surfaces transparent samples with higher resolutions and lower interference. To obtain focusing resolution aperture diaphragm system, model computing diffracted optical energy distribution an asymmetric constructed, mathematical formulas are deduced determining system based on position first dark ring amplitude distribution. Optical simulations were performed under specific conditions; lateral δr was determined to be 0.68 μm, accuracy δz 0.60 μm. An experimental platform established same conditions, results in accord those simulation results, which validated correctness formula calculating light from diaphragm.

Language: Английский

Citations

0
Resolution enhancement with deblurring by pixel reassignment (DPR) DOI Creative Commons
Bingying Zhao, Jérôme Mertz

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 24, 2023

Improving the spatial resolution of a fluorescence microscope has been an ongoing challenge in imaging community. To address this challenge, variety approaches have taken, ranging from instrumentation development to image post-processing. An example latter is deconvolution, where images are numerically deblurred based on knowledge point spread function. However, deconvolution can easily lead noise-amplification artifacts. Deblurring by post-processing also negativities or fail conserve local linearity between sample and image. We describe here simple deblurring algorithm pixel reassignment that inherently avoids such artifacts be applied general modalities fluorophore types. Our helps distinguish nearby fluorophores even when these separated distances smaller than conventional limit, helping facilitate, for example, application single-molecule localization microscopy dense samples. demonstrate versatility performance our under conditions.

Language: Английский

Citations

0