Measurement and analysis of rare circulating tumor cell dynamics with diffuse in vivo flow cytometry DOI Open Access

Amber Luna Williams

Published: Jan. 1, 2024

Circulating tumor cells (CTCs) are shed from primary tumors to facilitate hematogenous metastasis. The method of studying CTCs is by analysis small, drawn blood samples, known as 'liquid biopsy'. However, little about fluctuations in the number these over time. Our lab recently developed diffuse vivo flow cytometry (DiFC) which continuously and non-invasively scans large vessels small animals detect rare circulating fluorescent cells. aim this dissertation was develop new DiFC technology methods apply study variability CTC numbers different time scales.First, we analyzed data sets measured multiple myeloma Lewis lung carcinoma mouse metastasis models vivo. continuous nature allows us equate detections short intervals captured samples. showed that samples unlikely accurately estimate burden across entire volume. Additionally, short-term variation much higher than previously assumed, suggesting shedding rates change course minutes or hours. Second, designed, built, validated a system can with two distinct fluorophores, for example green protein (GFP) tdTomato protein. This monitor populations concurrently same animal two-fluorophore clusters containing both populations. Finally, applied two-color multi-fluorophore breast cancer bearing mice. These increased grew, but growth not monotonic Both exhibited similar variation, demonstrating types have unknown behavior. Whole-body hyperspectral fluorescence cryo-imaging also used identify volume, location, distribution metastases. methodology has significant implications understanding dissemination further interpretation liquid biopsy pre-clinical research humans.--Author's abstract

Language: Английский

Introduction to the feature issue: Advances in Optical Biosensors for Biomedical Applications DOI Creative Commons
Santosh Kumar, Agostino Iadicicco, Seunghyun Kim

et al.

Biomedical Optics Express, Journal Year: 2024, Volume and Issue: 15(5), P. 3183 - 3183

Published: April 15, 2024

The feature issue of

Language: Английский

Citations

5
Treatment of Acute Liver Injury through Selective Tropism of High Mobility Group Box 1 Gene-Silenced Large Peritoneal Macrophages DOI Creative Commons

Dhaval Oza,

Fernando Ivich,

Kirsten Deprey

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Tissue-resident macrophages (TRMs) are attractive cells to therapeutically deliver oligonucleotide and other gene-expression modifying modalities treat a wide array of diseases ranging from inflammatory autoimmune, even cancer. Here, we focus on TRMs located inside the peritoneal cavity lining abdomen that selectively express transcription factor GATA6 called large (GLPMs) successfully demonstrate functional GLPM-selective delivery Cy5-fluorophore-labeled siRNA encapsulated in C12–200 cationic-lipidoid-based nanoparticles (siRNA-Cy5 (C12–200)). Despite being TRMs, GLPMs possess specific migratory ability peritoneally liver tissue upon injury incited by acetaminophen (APAP) overdose mice. A rapid, injury-driven tropism carrying siRNA-Cy5 (C12–200) was seen via systemic circulation, which elegantly demonstrated using noninvasive live-cell tracking technique diffuse vivo flow cytometry (DiFC). Finally, RNAi-mediated silencing well-known pro-inflammatory damage-associated molecular pattern (DAMP) High Mobility Group Box-1 (HMGB1) gene led mitigation inflammation prevention GLPM modulation state, further translated into significant protection APAP-driven reduction circulating cytokines owing muted response acute injury. Moreover, HMGB1 GalNAc-conjugated hepatocyte-targeting did not reciprocate findings, solidifying our results. Together, data suggested act as carriers rapidly bringing lipid nanoparticle-encapsulated RNAi injured have emerged viable strategy address diseases, especially those more nature.

Language: Английский

Citations

0
Ratiometric fluorescence sensing and quantification of circulating blood sodium sensors in mice in vivo DOI Creative Commons
Fernando Ivich, Isen Andrew C. Calderon, Qianqian Fang

et al.

Biomedical Optics Express, Journal Year: 2023, Volume and Issue: 14(11), P. 5555 - 5555

Published: Sept. 26, 2023

In this work, we introduce ratiometric diffuse in vivo flow cytometry (R-DiFC) for quantitative measurement of circulating fluorescent red blood cell (fRBC) sensors systemic sodium levels. Unlike our previous work measuring fRBC sensors, R-DiFC allows simultaneous two fluorophores encapsulated the sensor, ratio which enables self-calibration fluorescence signal with different depths biological tissue. We show that varies significantly less than either alone. This holds promise personalized monitoring bipolar patients future.

Language: Английский

Citations

5
Introduction to the Optical Molecular Probes, Imaging and Drug Delivery 2023 feature issue DOI Creative Commons
Mark Niedre,

Kimberly S. Samkoe,

Susanne Kossatz

et al.

Biomedical Optics Express, Journal Year: 2024, Volume and Issue: 15(5), P. 3092 - 3092

Published: April 11, 2024

A feature issue is being presented by a team of guest editors containing papers based on studies at the Optical Molecular Probes, Imaging and Drug Delivery conference as part Optica Biophotonics Congress in Vancouver, Canada from April 24–27, 2023.

Language: Английский

Citations

0
Measurement and analysis of rare circulating tumor cell dynamics with diffuse in vivo flow cytometry DOI Open Access

Amber Luna Williams

Published: Jan. 1, 2024

Circulating tumor cells (CTCs) are shed from primary tumors to facilitate hematogenous metastasis. The method of studying CTCs is by analysis small, drawn blood samples, known as 'liquid biopsy'. However, little about fluctuations in the number these over time. Our lab recently developed diffuse vivo flow cytometry (DiFC) which continuously and non-invasively scans large vessels small animals detect rare circulating fluorescent cells. aim this dissertation was develop new DiFC technology methods apply study variability CTC numbers different time scales.First, we analyzed data sets measured multiple myeloma Lewis lung carcinoma mouse metastasis models vivo. continuous nature allows us equate detections short intervals captured samples. showed that samples unlikely accurately estimate burden across entire volume. Additionally, short-term variation much higher than previously assumed, suggesting shedding rates change course minutes or hours. Second, designed, built, validated a system can with two distinct fluorophores, for example green protein (GFP) tdTomato protein. This monitor populations concurrently same animal two-fluorophore clusters containing both populations. Finally, applied two-color multi-fluorophore breast cancer bearing mice. These increased grew, but growth not monotonic Both exhibited similar variation, demonstrating types have unknown behavior. Whole-body hyperspectral fluorescence cryo-imaging also used identify volume, location, distribution metastases. methodology has significant implications understanding dissemination further interpretation liquid biopsy pre-clinical research humans.--Author's abstract

Language: Английский

Citations

0