Dynamic Measurement of Flowing Microparticles in Microfluidics Using Pulsed Modulated Digital Holographic Microscopy

Photonics, Journal Year: 2025, Volume and Issue: 12(5), P. 411 - 411

Published: April 24, 2025

We propose a pulsed modulated digital holographic microscopy (PM-DHM) technique for the dynamic measurement of flowing microparticles in microfluidic systems. By digitally tuning pulse width and repetition rate laser source within single-frame exposure, this method enables recording multiple images at different time points single hologram, allowing quantification velocity acceleration. demonstrate feasibility PM-DHM by measuring velocity, acceleration, forces exerted on PMMA microspheres red blood cells chips. Compared to traditional frame-sampling-based imaging methods, has much higher resolution (in range microseconds) that is limited only duration. This demonstrates significant potential high-throughput label-free flow cytometry detection offers promising applications drug development cell analysis.

Language: Английский

Sensitive detection of K-ras gene by a dual-mode “on-off-on” sensor based on bipyridine ruthenium-MOF and bis-enzymatic cleavage technology

Bioelectrochemistry, Journal Year: 2024, Volume and Issue: 161, P. 108845 - 108845

Published: Nov. 5, 2024

Language: Английский