Dynamic Measurement of Flowing Microparticles in Microfluidics Using Pulsed Modulated Digital Holographic Microscopy DOI Creative Commons
Yunze Lei, Y. Li, Xiaofang Wang

et al.

Photonics, Journal Year: 2025, Volume and Issue: 12(5), P. 411 - 411

Published: April 24, 2025

We propose a pulsed modulated digital holographic microscopy (PM-DHM) technique for the dynamic measurement of flowing microparticles in microfluidic systems. By digitally tuning pulse width and repetition rate laser source within single-frame exposure, this method enables recording multiple images at different time points single hologram, allowing quantification velocity acceleration. demonstrate feasibility PM-DHM by measuring velocity, acceleration, forces exerted on PMMA microspheres red blood cells chips. Compared to traditional frame-sampling-based imaging methods, has much higher resolution (in range microseconds) that is limited only duration. This demonstrates significant potential high-throughput label-free flow cytometry detection offers promising applications drug development cell analysis.

Language: Английский

Recent Developments in SERS Microfluidic Chips: From Fundamentals to Biosensing Applications DOI
Shuai Lian, Xiaoqiong Li,

Xuefei Lv

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

This paper reviews the latest research progress of surface-enhanced Raman spectroscopy (SERS) microfluidic chips in field biosensing. Due to its single-molecule sensitivity, selectivity, minimal or no preprocessing, and immediacy, SERS is considered a promising biosensing technology. However, nondirectional interactions between biological samples substrate, as well fluctuations sample environment temperature during signal acquisition, can affect stability reproducibility signals. Integrating with not only leverages continuous flow, high reaction efficiency, throughput, multifunctionality address challenges applications but also expands scope technology by providing novel on-chip optical detection method. The combination enables complementary advantages both technologies offers highly "combined technology" for starts introducing enhancement mechanisms presents labeled label-free strategies. Based on differences substrate properties, we broadly categorize into colloidal nanoparticle-based fixed substrate-based chips. Finally, review targets such nucleic acids, proteins, small biomolecules, live cells. In conclusion outlook section, summarize faced propose feasible solutions. To better leverage role biosensing, present an future development this combined

Language: Английский

Citations

0
Dynamic Measurement of Flowing Microparticles in Microfluidics Using Pulsed Modulated Digital Holographic Microscopy DOI Creative Commons
Yunze Lei, Y. Li, Xiaofang Wang

et al.

Photonics, Journal Year: 2025, Volume and Issue: 12(5), P. 411 - 411

Published: April 24, 2025

We propose a pulsed modulated digital holographic microscopy (PM-DHM) technique for the dynamic measurement of flowing microparticles in microfluidic systems. By digitally tuning pulse width and repetition rate laser source within single-frame exposure, this method enables recording multiple images at different time points single hologram, allowing quantification velocity acceleration. demonstrate feasibility PM-DHM by measuring velocity, acceleration, forces exerted on PMMA microspheres red blood cells chips. Compared to traditional frame-sampling-based imaging methods, has much higher resolution (in range microseconds) that is limited only duration. This demonstrates significant potential high-throughput label-free flow cytometry detection offers promising applications drug development cell analysis.

Language: Английский

Citations

0