Polarization Gratings Aided Common‐Path Hilbert Holotomography for Label‐Free Lipid Droplets Content Assay
Laser & Photonics Review,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 13, 2025
Abstract
In
this
contribution
a
novel
polarization
gratings
aided
common‐path
Hilbert
holotomography
(CP‐HHT)
is
presented
for
label‐free
3D
refractive
index
imaging.
Addressing
limitations
in
current
methods,
the
extended
space‐bandwidth
product
leveraged
through
robust
phase
demodulation
using
spiral
transform.
The
application
of
diffraction
system
enables
fully
tailored
holographic
settings
such
as
fringe
density
and
shear,
allowing
flexible
hologram
demodulation,
while
maintaining
simplicity
robustness.
performance
tested
3D‐printed
(fabricated
with
two‐photon
polymerization)
brain
phantom
fixed
HeLa
cells
supplemented
cholesterol
oleic
acids.
Reconstruction
analysis
indicates
that
method
provides
improved
spatial
resolution
to
Fourier
transform
significantly
expanded
measurement
information
content.
This
CP‐HHT
approach
demonstrates
unique
(not
possible
by
fluorescence)
ability
measure
lipid
droplets
enriched
acid
highlights
they
exhibit
measurable
differences
their
values.
These
findings
suggest
sensitive
variations
neutral
content,
offering
promising
insights
into
droplet
heterogeneity
supporting
its
potential
sub‐cellular
bioimaging
applications,
thus
reinforcing
versatility
applicability
broader
applications.
Language: Английский
Quantitative Phase Imaging (QPI): Introduction to the feature issue in Journal of the Optical Society of America A
Journal of the Optical Society of America A,
Journal Year:
2024,
Volume and Issue:
41(11), P. QPI1 - QPI1
Published: Oct. 22, 2024
Quantitative
phase
imaging
(QPI),
propelled
by
advancements
in
digital
holography
and
computational
imaging,
has
revolutionized
the
ability
to
retrieve
delays
with
high
precision.
Over
past
two
decades,
field
seen
tremendous
growth,
contributing
numerous
applications
biomedicine
material
metrology,
including
live
cell
monitoring,
structure
profiling,
defect
inspection,
among
others.
This
special
issue
commemorates
Prof.
Gabriel
"Gabi"
Popescu,
a
former
faculty
member
at
University
of
Illinois
Urbana-Champaign
pioneer
QPI
label-free
biological
who
passed
away
on
June
16,
2022,
his
hometown
Prundu,
Romania.
The
honors
Gabi's
legacy
collection
articles
exploring
methodologies
their
diverse
applications.
Language: Английский