Frontiers in Optics + Laser Science 2022 (FIO, LS), Journal Year: 2023, Volume and Issue: unknown, P. FM5E.5 - FM5E.5
Published: Jan. 1, 2023
This study presents recent advancements in stimulated Brillouin microscopy by utilizing a pulsed scheme and extracting first order harmonics to achieve 100-fold signal-to-noise ratio (SNR) enhancement. Here, we demonstrate the application of signal processing method leads an additional two-fold improvement SNR.
Language: Английский
Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)
Published: Feb. 1, 2024
Language: Английский
Citations
24
Photoacoustics, Journal Year: 2025, Volume and Issue: 42, P. 100696 - 100696
Published: Feb. 10, 2025
Brillouin microscopy has been widely used in the mechanical imaging of cells and tissues, signal-to-noise (SNR) ratio limits spectral integration time. Impulsive stimulated scattering (ISBS) is a new elastic technique. As variant (SBS), ISBS can overcome weak signal spontaneous scattering. A simple model estimate SBS gain. However, theoretical gain not compared with This paper gives estimation, experiments are designed to verify estimation reliability. The heterodyne coefficient be much higher than coefficient. relationship between spatial resolution then discussed. We anticipate that setup optimization improve gain, potentially enabling fast high biological cells.
Language: Английский
Citations
0
Nature Photonics, Journal Year: 2025, Volume and Issue: 19(5), P. 494 - 501
Published: Feb. 20, 2025
Abstract Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a three-dimensional, all-optical and hence non-contact fashion. However, the low cross-section spontaneous scattering produces weak signals often necessitate prolonged exposure times or illumination dosages are potentially harmful for samples. Here we present new approach highly multiplexed therefore rapid spectral acquisition Brillouin-scattered light. Specifically, by exploiting custom-built Fourier-transform imaging spectrometer symmetric spectrum, experimentally demonstrate full-field 2D phantoms as well samples, at throughput up 40,000 spectra per second, with precision ~70 MHz effective image speed 0.1 Hz over ~300 × 300 µm 2 field view. This represents approximately three-orders-of-magnitude improvement compared standard confocal methods, while retaining high spatial resolution capability acquire three-dimensional images photosensitive biology medicine.
Language: Английский
Citations
0
Journal of Physics Photonics, Journal Year: 2024, Volume and Issue: 6(3), P. 035016 - 035016
Published: May 27, 2024
Abstract Brillouin spectroscopy has recently attracted attention as a powerful tool for the characterization of mechanical properties heterogeneous materials, particularly in biological and biomedical domains. This study investigates procedure to use data provide relevant morphological parameters micro-structured samples. When acquiring spectra at interface between two regions sample, spectrum shows signatures both regions. feature can be used precisely identify position interfaces by analyzing evolution fitting acquired performing linear scan across interface. concept been demonstrated measuring thickness adherent HEK293T cells. The results are validated using fluorescence microscopy, showing an excellent agreement. present analysis showcases wealth information potentiality not only but also label-free, high-resolution imaging sample morphology. introduces possibility correlating shape samples single technique.
Language: Английский
Citations
2
Laser & Photonics Review, Journal Year: 2024, Volume and Issue: 18(6)
Published: Feb. 5, 2024
Abstract Structured light with multiple degrees of freedom has inspired many advanced applications, and its nonlinear control plays an important role in the interactions between matter. Stimulated Brillouin scattering (SBS) based on light–sound coupling can be used to realize wavefront‐reversal reflection ordinary light; consequently, it is correct wavefront aberrations high‐power laser systems. However, high‐fidelity reversal structured orbital angular momentum high‐reflectivity single‐end SBS weak incident waves remain challenging. Here, authors propose a cross‐pump focused scheme that enables high fidelity, low threshold, reflectivity for arbitrary . The demonstrate capability this approach perform conformal enhanced reflections fidelity >95% >100%. Compared conventional configuration, threshold reduced by more than ten times, weakly realized. This proof‐of‐principle study provides high‐performance platform potential implications lasers, signal‐to‐noise‐ratio microscopy imaging, development optics light.
Language: Английский
Citations
1
Nature Photonics, Journal Year: 2024, Volume and Issue: unknown
Published: June 10, 2024
Language: Английский
Citations
1
Published: March 12, 2024
Language: Английский
Citations
0
Journal of Physics Photonics, Journal Year: 2024, Volume and Issue: 6(3), P. 035004 - 035004
Published: May 2, 2024
Abstract Impulsive stimulated Brillouin scattering (ISBS) is a variant of scattering, which can overcome the shortcomings long acquisition time traditional microscopy. We introduce difference between ISBS and other microscopies in calculating longitudinal modulus. The frequency shift obtained by only related to system parameters speed sound (SOS) sample, not refractive index. Non-contact SOS measurement homogeneous samples an important application used early study spectroscopy mechanical properties liquids. However, requires prior knowledge sample index, limits unknown index sample. Here, we propose method measure based on ISBS, principle avoids need for parameters. several liquids are measured compared with standard values. mean relative deviation 1.13%. Moreover, mixture ethanol water demonstrate measuring without information. also high spatial resolution methanol-filled PDMS
Language: Английский
Citations
0
Journal of Physics Photonics, Journal Year: 2024, Volume and Issue: 6(3), P. 032001 - 032001
Published: June 6, 2024
Abstract Stimulated Brillouin scattering (SBS) microscopy is a nonlinear all-optical imaging method that provides mechanical contrast based on the interaction of laser radiation and acoustical vibrational modes. Featuring high specificity sensitivity, three-dimensional sectioning, practical times, SBS with (quasi) continuous wave excitation rapidly advancing as promising tool for label-free visualization viscoelastic information materials living biological systems. In this article, we introduce theory review current state-of-the-art well recent innovations, including different approaches to system designs data analysis. particular, various performance parameters its applications in life sciences are described discussed. Future perspectives also presented.
Language: Английский
Citations
0
Journal of Physics Photonics, Journal Year: 2024, Volume and Issue: 6(3), P. 035024 - 035024
Published: June 25, 2024
Abstract Brillouin spectroscopy is commonly used to study the acoustic properties of materials. Here we explored its feasibility in studying photoinduced isomerization azobenzene. The azobenzene changes solution elastic modulus, and scattering sensitive these changes. In this study, experimentally demonstrated photoswitching DMSO using our home-made virtually imaged phased array-based high-resolution optical spectrometer, confirmed results by ultraviolet–visible spectrophotometry. Remarkable frequency shift variations were quantitatively recorded upon irradiation, it was found that method can indeed be monitor process situ . Importantly, strategy also allows us provide relationship between fraction trans- cis- shift. This shows has broad prospects for characterization other photoresponsive
Language: Английский
Citations
0