Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy for rapid, label-free biological imaging DOI
Jessica Z. Dixon, Wei‐Wen Chen, Haoyu Xu

et al.

Review of Scientific Instruments, Journal Year: 2025, Volume and Issue: 96(4)

Published: April 1, 2025

Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy is a label-free imaging approach that provides detailed chemical information at high spatial resolution in sample through nonlinear, excitation of molecular vibrations and detection spectra. While its utility for biological has been demonstrated, many aspects this technique must mature before it can be widely adopted. One the areas required improvement speed—most BCARS implementations involve rastering, which limits speed. Beam scanning provide faster but presents some unique challenges. Here, we describe beam-scanning system improves twofold speed by fivefold over previous implementation. These enhancements were enabled an supercontinuum power use sCMOS camera data transfer rate low read noise. Implementation correction significant pixel-to-pixel background photon response nonuniformity. We report on method implemented calibrating correcting differences

Language: Английский

Design and Analysis of Spaceborne Hyperspectral Imaging System for Coastal Studies DOI Creative Commons
Yin Wu, Yueming Wang, Dong Zhang

et al.

Remote Sensing, Journal Year: 2025, Volume and Issue: 17(6), P. 986 - 986

Published: March 11, 2025

Hyperspectral payloads with high spatial and spectral resolution, combined a wide field of view, are crucial for tackling the complexity coastal estuarine water ecosystems, enabling effective monitoring quality ecological conditions. This study introduces modular spectrometer design utilizing multiple sub-modules in an extended slit configuration. The system delivers resolution 5 nm (400–1000 nm) 10 (1000–2500 nm), 20 m, swath width 80 km. Smile keystone distortions maintained below 1/5 pixel. Using Modran to simulate solar irradiance, SNR different targets under typical background conditions is calculated. Compared conventional designs, proposed approach provides compactness fidelity, effectively addressing size optical aberration challenges. simulation results confirm system’s robustness, setting benchmark next-generation coast observation missions, particularly monitoring, underwater exploration, dynamic environmental change tracking.

Language: Английский

Citations

0
Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy for rapid, label-free biological imaging DOI
Jessica Z. Dixon, Wei‐Wen Chen, Haoyu Xu

et al.

Review of Scientific Instruments, Journal Year: 2025, Volume and Issue: 96(4)

Published: April 1, 2025

Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy is a label-free imaging approach that provides detailed chemical information at high spatial resolution in sample through nonlinear, excitation of molecular vibrations and detection spectra. While its utility for biological has been demonstrated, many aspects this technique must mature before it can be widely adopted. One the areas required improvement speed—most BCARS implementations involve rastering, which limits speed. Beam scanning provide faster but presents some unique challenges. Here, we describe beam-scanning system improves twofold speed by fivefold over previous implementation. These enhancements were enabled an supercontinuum power use sCMOS camera data transfer rate low read noise. Implementation correction significant pixel-to-pixel background photon response nonuniformity. We report on method implemented calibrating correcting differences

Language: Английский

Citations

0