Detection and characterization of colorectal cancer by autofluorescence lifetime imaging: a preliminary study on surgical specimens DOI Creative Commons
Alberto Ignacio Herrando, Laura M. Fernández, José Azevedo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 30, 2024

Abstract Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report of ex vivo autofluorescence lifetime fingerprint colorectal tissues obtained from 73 patients that underwent surgical resection. specifically target characteristics collagens, reduced nicotine adenine (phosphate) dinucleotide (NAD(P)H), and flavins employing fiber-based dual excitation (375 nm 445 nm) optical imaging system. Autofluorescence-derived parameters normal tissues, adenomatous lesions, adenocarcinomas were analyzed considering underlying clinicopathological features. Our results indicate differences between are primarily driven by collagen parameters. also changes in associated with NAD(P)H we tentatively attribute to intratumoral heterogeneity, potentially presence distinct metabolic subpopulations. Changes signatures tumors observed lymphatic venous invasion, differentiation grade, microsatellite instability. Finally, characterized impact radiative treatment fingerprints rectal generalized increase mean radiated tumors, which is suggestive altered metabolism structural remodeling. Overall, our preliminary findings multiparametric measurements have potential significantly enhance clinical decision-making CRC, spanning initial diagnosis ongoing management. believe will provide foundational framework future investigations further understand combat CRC exploiting measurements.

Language: Английский

Fluorescence Lifetime Imaging Techniques—A Review on Principles, Applications and Clinical Relevance DOI Creative Commons
Vladislav I. Shcheslavskiy, Marina V. Shirmanova, Konstantin Yashin

et al.

Journal of Biophotonics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

ABSTRACT This article gives an overview of the most frequently used fluorescence‐lifetime imaging (FLIM) techniques, their capabilities, and typical applications. Starting from a general introduction to fluorescence phosphorescence lifetime, we will show that lifetime or, more accurately, decay function fluorophore is direct indicator interaction with its molecular environment. FLIM therefore than simple contrast technique in microscopy—it imaging. techniques can be classified into time‐domain frequency‐domain analogue photon counting scanning wide‐field techniques. these technical principles describe features peculiarities different use. An extended section dedicated TCSPC FLIM, addressing unique capabilities make especially interesting biological systems.

Language: Английский

Citations

1
Towards Optical Biopsy in Glioma Surgery DOI Open Access
Konstantin Yashin, Vladislav I. Shcheslavskiy, Igor Medyanik

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4554 - 4554

Published: May 9, 2025

Currently, the focus of intraoperative imaging in brain tumor surgery is beginning to shift optical methods such as coherence tomography (OCT), Raman spectroscopy, confocal laser endomicroscopy (CLE), and fluorescence lifetime (FLIM). Optical technologies provide vivo real-time high-resolution images tissues. “Optical biopsy” can be considered an alternative traditional approaches for histopathologic consultation. Intraoperative help achieve precise identification infiltrations within surrounding parenchyma. Therefore, it a complement existing based on wide-field modalities MRI, US, or 5-ALA fluorescence. A promising future direction guidance during stereotactic biopsy lies integration with machine learning techniques, enabling automated differentiation between tissue healthy We present this review increase knowledge form critical opinions field using surgery.

Language: Английский

Citations

0
Simultaneous assessment of NAD(P)H and flavins with multispectral fluorescence lifetime imaging microscopy at a single excitation wavelength of 750 nm DOI Creative Commons
Boris P. Yakimov, Anastasia D. Komarova, Elena E Nikonova

et al.

Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(10)

Published: Sept. 30, 2024

Autofluorescence characteristics of the reduced nicotinamide adenine dinucleotide and oxidized flavin cofactors are important for evaluation metabolic status cells. The approaches that involve a detailed analysis both spectral time autofluorescence signals may provide additional insights into biochemical processes in cells biological tissues facilitate transition fluorescence lifetime imaging clinical applications.

Language: Английский

Citations

1
Detection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimens DOI Creative Commons
Alberto Ignacio Herrando, Laura M. Fernández, José Azevedo

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 19, 2024

Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report of ex vivo autofluorescence lifetime fingerprint colorectal tissues obtained from 73 patients that underwent surgical resection. specifically target characteristics collagens, reduced nicotine adenine (phosphate) dinucleotide (NAD(P)H), and flavins employing fiber-based dual excitation (375 nm 445 nm) optical imaging system. Autofluorescence-derived parameters normal tissues, adenomatous lesions, adenocarcinomas were analyzed considering underlying clinicopathological features. Our results indicate differences between are primarily driven by collagen parameters. also changes in associated with NAD(P)H we tentatively attribute to intratumoral heterogeneity, potentially presence distinct metabolic subpopulations. Changes signatures malignant tumors observed lymphatic venous invasion, differentiation grade, microsatellite instability. Finally, characterized impact radiative treatment fingerprints rectal generalized increase mean radiated adenocarcinomas, which is suggestive altered metabolism structural remodeling. Overall, our preliminary findings multiparametric measurements have potential significantly enhance clinical decision-making CRC, spanning initial diagnosis ongoing management. believe will provide foundational framework future investigations further understand combat CRC exploiting measurements.

Language: Английский

Citations

1
Detection and characterization of colorectal cancer by autofluorescence lifetime imaging: a preliminary study on surgical specimens DOI Creative Commons
Alberto Ignacio Herrando, Laura M. Fernández, José Azevedo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 30, 2024

Abstract Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report of ex vivo autofluorescence lifetime fingerprint colorectal tissues obtained from 73 patients that underwent surgical resection. specifically target characteristics collagens, reduced nicotine adenine (phosphate) dinucleotide (NAD(P)H), and flavins employing fiber-based dual excitation (375 nm 445 nm) optical imaging system. Autofluorescence-derived parameters normal tissues, adenomatous lesions, adenocarcinomas were analyzed considering underlying clinicopathological features. Our results indicate differences between are primarily driven by collagen parameters. also changes in associated with NAD(P)H we tentatively attribute to intratumoral heterogeneity, potentially presence distinct metabolic subpopulations. Changes signatures tumors observed lymphatic venous invasion, differentiation grade, microsatellite instability. Finally, characterized impact radiative treatment fingerprints rectal generalized increase mean radiated tumors, which is suggestive altered metabolism structural remodeling. Overall, our preliminary findings multiparametric measurements have potential significantly enhance clinical decision-making CRC, spanning initial diagnosis ongoing management. believe will provide foundational framework future investigations further understand combat CRC exploiting measurements.

Language: Английский

Citations

0