Deep two-photon voltage imaging with adaptive excitation DOI
Shitong Zhao, Eric Hébert, Anna M. Gruzdeva

et al.

Published: Jan. 1, 2024

We apply adaptive excitation to a fast two-photon microscope image voltage activity in multiple neurons simultaneously at > 600 μm depth awake mouse brains.

Language: Английский

Multiphoton fluorescence microscopy for in vivo imaging DOI
Chris Xu, Maiken Nedergaard, Deborah J. Fowell

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(17), P. 4458 - 4487

Published: Aug. 1, 2024

Language: Английский

Citations

21
EventLFM: event camera integrated Fourier light field microscopy for ultrafast 3D imaging DOI Creative Commons
Ruipeng Guo,

Qianwan Yang,

Andrew S. Chang

et al.

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: June 26, 2024

Abstract Ultrafast 3D imaging is indispensable for visualizing complex and dynamic biological processes. Conventional scanning-based techniques necessitate an inherent trade-off between acquisition speed space-bandwidth product (SBP). Emerging single-shot wide-field offer a promising alternative but are bottlenecked by the synchronous readout constraints of conventional CMOS systems, thus restricting data throughput to maintain high SBP at limited frame rates. To address this, we introduce EventLFM, straightforward cost-effective system that overcomes these challenges integrating event camera with Fourier light field microscopy (LFM), state-of-the-art technique. The operates on novel asynchronous architecture, thereby bypassing rate limitations systems. We further develop simple robust event-driven LFM reconstruction algorithm can reliably reconstruct dynamics from unique spatiotemporal measurements captured EventLFM. Experimental results demonstrate EventLFM robustly fast-moving rapidly blinking fluorescent samples kHz Furthermore, highlight EventLFM’s capability neuronal signals in scattering mouse brain tissues tracking GFP-labeled neurons freely moving C. elegans . believe combined ultrafast large offered may open up new possibilities across many biomedical applications.

Language: Английский

Citations

8
Exploring the future of regenerative medicine: Unveiling the potential of optical microscopy for structural and functional imaging of stem cells DOI Creative Commons
Gagan Raju, Smitha Nayak,

Neha Acharya

et al.

Journal of Biophotonics, Journal Year: 2024, Volume and Issue: 17(1)

Published: Jan. 1, 2024

Regenerative medicine, which utilizes stem cells for tissue and organ repair, holds immense promise in healthcare. A comprehensive understanding of cell characteristics is crucial to unlock their potential. This study explores the pivotal role optical microscopy advancing regenerative medicine as a potent tool research. Advanced techniques enable an in-depth examination behavior, morphology, functionality. The review encompasses current microscopy, elucidating its capabilities constraints imaging, while also shedding light on emerging technologies improved visualization. Optical complemented by like fluorescence multiphoton enhances our comprehension dynamics. introduction label-free imaging facilitates noninvasive, real-time monitoring without external dyes or markers. By pushing boundaries researchers reveal intricate cellular mechanisms underpinning processes, thereby more effective therapeutic strategies. not only outlines future but underscores both structural functional imaging.

Language: Английский

Citations

5
Compressive sensing-based multi-focus line-scanning two-photon microscopy for fast 3D imaging DOI Creative Commons
Gan Liu, Bingxu Chen,

Zezhi Tian

et al.

Optics Express, Journal Year: 2024, Volume and Issue: 32(10), P. 17143 - 17143

Published: April 5, 2024

Fast 3D volume imaging methods have been playing increasingly important roles in biological studies. In this article, we present the design and characterization of a multi-focus line-scanning two-photon microscope. Specifically, digital micromirror device (DMD) is employed to generate randomly distributed focus array on plane (i.e., x-z) via binary holography. Next, galvanometric mirror scans direction normal y-axis) over volume. For sparse samples, e.g., neural networks brain, 1-3 foci are used together with compressive sensing algorithm achieve rate 15.5 volumes/sec 77 × 120 40 µm

Language: Английский

Citations

2
Bessel Beam Optical Coherence Microscopy Enables Multiscale Assessment of Cerebrovascular Network Morphology and Function DOI Open Access
Lukas Glandorf, Bastian Wittmann,

Jeanne Droux

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 20, 2024

Abstract Understanding the morphology and function of large-scale cerebrovascular networks is crucial for studying brain health disease. However, reconciling demands imaging on a broad scale with precision high-resolution volumetric microscopy has been persistent challenge. In this study, we introduce Bessel beam optical coherence an extended focus to capture full cortical vascular hierarchy in mice over 1000 × 360 μm 3 field-of-view at capillary level resolution. The post-processing pipeline leverages supervised deep learning approach precise 3D segmentation angiograms, hence permitting reliable examination microvascular structures multiple spatial scales. Coupled high-sensitivity Doppler tomography, our method enables computation both axial transverse blood velocity components as well vessel-specific flow direction, facilitating detailed assessment morpho-functional characteristics across all vessel dimensions. Through graph-based analysis, deliver insights into connectivity, way from individual capillaries broader network interactions, task traditionally challenging vivo studies. new analysis framework extends frontiers research neurovascular pathologies.

Language: Английский

Citations

1
Bessel beam optical coherence microscopy enables multiscale assessment of cerebrovascular network morphology and function DOI Creative Commons
Lukas Glandorf, Bastian Wittmann,

Jeanne Droux

et al.

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 11, 2024

Understanding the morphology and function of large-scale cerebrovascular networks is crucial for studying brain health disease. However, reconciling demands imaging on a broad scale with precision high-resolution volumetric microscopy has been persistent challenge. In this study, we introduce Bessel beam optical coherence an extended focus to capture full cortical vascular hierarchy in mice over 1000 × 360 μm

Language: Английский

Citations

1
High throughput detection of capillary stalling events with Bessel beam two-photon microscopy DOI Creative Commons
John T. Giblin, Sreekanth Kura,

Juan Luis Ugarte Nunuez

et al.

Neurophotonics, Journal Year: 2023, Volume and Issue: 10(03)

Published: Sept. 12, 2023

Brief disruptions in capillary flow, commonly referred to as "stalling," have gained interest recently for their potential role disrupting cerebral blood flow and oxygen delivery. Approaches studying this phenomenon been hindered by limited volumetric imaging rates cumbersome manual analysis. The ability precisely efficiently quantify the dynamics of these events will be key understanding stroke neurodegenerative diseases, such Alzheimer's disease.

Language: Английский

Citations

3
Optical segmentation-based compressed readout of neuronal voltage dynamics DOI Creative Commons
Seonghoon Kim,

Gwanho Ko,

Iksung Kang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 13, 2023

Abstract Functional imaging of biological dynamics generally begins with acquiring time-series images, followed by quantifying spatially averaged intensity traces for the regions interest (ROIs). The conventional pipeline discards a substantial portion acquired data when traces, indicative inefficient acquisition. Here we propose conceptually novel acquisition that assigns each ROI to single pixel in detector, enabling optimally compressed traces. As proof-of-principle, implemented detection module composed pair spatial light modulators and microlens array, which segments original image into multiple subimages introducing distinct angular shifts ROI. Each subimage exclusively encodes signal corresponding ROI, facilitating readout its trace using pixel. This compression allowed maximizing temporal information without compromising on ROIs. Harnessing our approach, demonstrate recording circuit-scale neuronal voltage at over 5 kHz sampling rate, revealing individual action potential waveforms within subcellular structures, as well their submillisecond-scale delays.

Language: Английский

Citations

2
Label-free image scanning microscopy for kHz super-resolution imaging and single particle tracking DOI Creative Commons

Duc-Minh Ta,

Alberto Aguilar, Pierre Bon

et al.

Optics Express, Journal Year: 2023, Volume and Issue: 31(22), P. 36420 - 36420

Published: Oct. 5, 2023

We report the modification of a label-free image scanning microscope (ISM) to perform asynchronous 2D imaging at up 24kHz while keeping lateral resolution gain and background rejection regular ISM setup. Our method uses resonant mirror oscillating 12kHz for one-direction chromatic line instantaneous in other direction. adapt optical photon reassignment this regime fully super-resolution imaging. exploit kHz capabilities confocal system single nanoparticle tracking down 20 nm gold 50 silica particles as well freely moving Lactobacillus with improved resolution.

Language: Английский

Citations

2
Suppression of Subpixel Jitter in Resonant Scanning Systems With Phase-locked Sampling DOI Creative Commons
Vincent D. Ching-Roa, Chi Z. Huang, Michael G. Giacomelli

et al.

IEEE Transactions on Medical Imaging, Journal Year: 2024, Volume and Issue: 43(6), P. 2159 - 2168

Published: Jan. 24, 2024

Resonant scanning is critical to high speed and in vivo imaging in many applications of laser microscopy. However, resonant suffers from well known image artifacts due scanner jitter, limiting adoption high-speed technologies. Here, we introduce a real-time, inexpensive all electrical method suppress jitter more than an order magnitude below the diffraction limit that can be applied most existing microscope systems with no software changes. By phase-locking period, demonstrate 86% reduction pixel 15% improvement point spread function show this approach enables two widely used models scanners achieve comparable accuracy galvanometer running orders slower. Finally, versatility by retrofitting commercial photon significant quantitative qualitative improvements biological imaging.

Language: Английский

Citations

0