Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants DOI Open Access
Michael J. Prigge, Nicholas Morffy,

A. Neve

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 13, 2023

A major challenge in plant biology is to understand how the hormone auxin regulates diverse transcriptional responses throughout development, different environments, and species. The answer may lie specific complement of signaling components each cell. balance between activators (class-A AUXIN RESPONSE FACTORS) repressors (class-B ARFs) particularly important. It unclear this achieved. Through comparative analysis novel, dominant mutants maize moss Physcomitrium patens , we have discovered a ∼500-million-year-old mechanism class-B ARF protein level regulation, important determining cell fate decisions across land plants. Thus, our results add key piece puzzle development.

Language: Английский

The ratio of auxin to cytokinin controls leaf development and meristem initiation in Physcomitrium patens DOI
Joseph Cammarata, Adrienne Roeder, Michael J. Scanlon

et al.

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 74(21), P. 6541 - 6550

Published: July 25, 2023

Crosstalk between auxin and cytokinin contributes to widespread developmental processes, including root shoot meristem maintenance, phyllotaxy, vascular patterning. However, our understanding of crosstalk these hormones is limited primarily angiosperms. The moss Physcomitrium patens (formerly Physcomitrella patens) a powerful system for studying plant hormone function. Auxin play similar roles in regulating gametophore (shoot) architecture, those flowering shoots. auxin-cytokinin poorly understood moss. Here we find that the ratio an important determinant development P. patens, especially during leaf branch stem cell initiation. Addition high levels gametophores blocks outgrowth. simultaneous addition both partially restores outgrowth, suggesting predominant factor. Likewise, initiation chemical inhibition synthesis phenocopies application. Finally, cytokinin-insensitive mutants resemble plants with altered signaling are hypersensitive auxin. In summary, results suggest basis decisions gametophore.

Language: Английский

Citations

15
Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants DOI Creative Commons
Michael J. Prigge, Nicholas Morffy,

A. Neve

et al.

Nature Plants, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Language: Английский

Citations

0
The role of auxin-mediated gene activation in the bryophyte, Physcomitrium patens DOI Creative Commons
Carlisle Bascom,

Domena K. Tu,

Mark Estelle

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Abstract Perception and response to the hormone auxin is critical plant growth development. Expression of auxin-response genes tightly regulated via known mechanisms both activation repression. Across lineage, gene induction performed by AUXIN-REPSONSE FACTOR (ARF) activating transcription factors. Conversely, AUXIN/INDOLE ACETIC ACID proteins repress expression. Studies gain-of-function constitutive-repression lines ARF loss-of-function mutants have advanced field. Yet, there a need for comparative study aberrant auxin-signaling understand developmental consequences constitutive repression versus absence auxin-mediated induction. Using CRISPR/Cas9 gene-editing tools, we mutated each in model bryophyte, Physcomitrium patens. The resulting septuple mutant line ( arfa sept ) has severe phenotypes diminished ability respond exogenous auxin. However, phenotypic analysis revealed that not as severely affected lines. several demonstrate abolished but basal expression levels are higher Our results suggest auxin-regulated important progression maintained, albeit at reduced levels, ARFs. Highlight Researchers used CRIPSR/Cas9 produce Auxin Response Factor bryophyte patens revealing complete loss on

Language: Английский

Citations

0
Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants DOI Open Access
Michael J. Prigge, Nicholas Morffy,

A. Neve

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 13, 2023

A major challenge in plant biology is to understand how the hormone auxin regulates diverse transcriptional responses throughout development, different environments, and species. The answer may lie specific complement of signaling components each cell. balance between activators (class-A AUXIN RESPONSE FACTORS) repressors (class-B ARFs) particularly important. It unclear this achieved. Through comparative analysis novel, dominant mutants maize moss Physcomitrium patens , we have discovered a ∼500-million-year-old mechanism class-B ARF protein level regulation, important determining cell fate decisions across land plants. Thus, our results add key piece puzzle development.

Language: Английский

Citations

1