Pseudomonas aeruginosa faces a fitness trade-off between mucosal colonization and antibiotic tolerance during airway infections

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract Pseudomonas aeruginosa causes antibiotic-resilient acute and chronic pneumonia, but the mechanisms by which it adapts to airway environment are poorly understood. Here, we investigated P. pathoadaptive in tissue-engineered human organoids. Using transposon sequencing situ, decoded how survives on mucosal surface during antibiotic treatment. Biofilm formation emerged as a major driver of colonization. Mutants that extensively produce biofilms mucus show limited exploratory behavior, limits nutrient access, slowing down their growth. Conversely, biofilm-dwelling better tolerate antibiotics via biophysical mechanisms. Finally, can shelter less-tolerant more cytotoxic strains, thereby contributing genotypic heterogeneity. must therefore adapt conflicting physical biological selective pressures initiate infections.

Language: Английский

Microtopographic Influence on Bacterial Biofilm Development in Habitat-Like Environments

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 106311 - 106311

Published: Oct. 1, 2024

Language: Английский

Tailored multilayer nanoparticles against resistant P. aeruginosa by disrupting the thickened mucus, dense biofilm and hyperinflammation

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 378, P. 588 - 604

Published: Dec. 25, 2024

Language: Английский