Experimental Reporting of Fish Transcriptomic Responses in Environmental Toxicology and Ecotoxicology

Environmental Toxicology and Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Due to its increasing affordability and efforts understand transcriptional responses of organisms biotic abiotic stimuli, transcriptomics has become an important tool with significant impact on toxicological investigations hazard risk assessments, especially during development application new approach methodologies (NAMs). Data generated using transcriptomic have directly informed adverse outcome pathway frameworks, chemical biological read across, aided in the identification points departure. Using data reporting frameworks for offers improved transparency reproducibility research opportunity identify barriers adoption these NAMs, environmental toxicology ecotoxicology aquatic models. Improved also allows reexamination existing data, limiting needs experiment replication further reducing animal experimentation. Here, we use a standardized form omics-based studies, Organisation Economic Co-operation Development omics framework, which specifically reports list parameters that should be included studies used regulatory context. We focused fish RNA- Sequencing (Seq)/microarray technologies within Inconsistencies among experimental designs (toxicology vs. molecular characterization) were observed addition foundational differences sample concentration or preparation quality can affect confidence results, contribute substantially understanding mechanisms toxicants toxins. Our findings present reporting. provide several recommendations as logical steps reduce ecotoxicology.

Language: Английский

---

Advancing the Spatiotemporal Dimension of Wildlife–Pollution Interactions

Environmental Science & Technology Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Chemical pollution is one of the fastest-growing agents global change. Numerous pollutants are known to disrupt animal behavior, alter ecological interactions, and shift evolutionary trajectories. Crucially, both chemical individual organisms nonrandomly distributed throughout environment. Despite this fact, current evidence for chemical-induced impacts on wildlife largely stems from tests that restrict organism movement force homogeneous exposures. While such approaches have provided pivotal ecotoxicological insights, they overlook dynamic spatiotemporal interactions shape wildlife-pollution relationships in nature. Indeed, seemingly simple notion animals move environment creates a complex many which never been theoretically modeled or experimentally tested. Here, we conceptualize between variation highlight their implications. We propose three-pronged approach-integrating silico modeling, laboratory experiments allow movement, field-based tracking free-ranging animals-to bridge gap controlled studies real-world Advances telemetry, remote sensing, computational models provide necessary tools quantify these paving way new era ecotoxicology accounts complexity.

Language: Английский

---

Latitude shapes diel patterns in insect biodiversity

Biology Letters, Journal Year: 2025, Volume and Issue: 21(4)

Published: April 1, 2025

The writings of naturalists from two centuries past are brimming with accounts the stark differences in kinds and numbers organisms encountered during day night as well between tropical temperate zones. However, only recently have ecologists begun to systematically explore geographic variation diel activity patterns species on Earth. Examining data 60 insect communities distributed globally, I find that proportion nocturnal a community declines peak 36% at equator 8% 60° latitude, while diurnal shows no significant trend. By contrast, cathemeral (day- night-active) increases poleward 18% 68% along same gradient. These latitudinal trends partitioning time among co-occurring broadly reflect previously documented biogeographic global distributions vertebrate occupying different temporal niches. Since shape dynamics, uncovering their mechanistic basis roles factors such temperature, light biotic interactions is vital for curbing Anthropocene.

Language: Английский

---

Timing Matters: Time of Day Impacts the Ergogenic Effects of Caffeine—A Narrative Review

Nutrients, Journal Year: 2024, Volume and Issue: 16(10), P. 1421 - 1421

Published: May 8, 2024

Caffeine has attracted significant attention from researchers in the sports field due to its well-documented ergogenic effects across various athletic disciplines. As research on caffeine continues progress, there been a growing emphasis evaluating dosage and administration methods. However, investigations into optimal timing of intake remain limited. Therefore, this narrative review aimed assess at different times during morning (06:00 10:00) evening (16:00 21:00). The findings suggest that circadian rhythms play substantial role influencing performance, potentially contributing decline performance. demonstrated effectiveness mitigating phenomenon, resulting performance enhancement, even comparable nighttime levels. While specific mechanisms by which regulates influences unclear, also explores underlying caffeine’s effects, including adenosine receptor blockade, increased muscle calcium release, modulation catecholamines. Additionally, underscores indirect impact enhancing responsiveness light-induced phase shifts. Although precise through improves declines via rhythm regulation necessitate further investigations, it is noteworthy significantly affects exercise. This emphasizes importance considering future endeavors optimize potential elucidate mechanisms.

Language: Английский

---

EthoCRED: a framework to guide reporting and evaluation of the relevance and reliability of behavioural ecotoxicity studies

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

ABSTRACT Behavioural analysis has been attracting significant attention as a broad indicator of sub‐lethal toxicity and secured place an important subdiscipline in ecotoxicology. Among the most notable characteristics behavioural research, compared to other established approaches ecotoxicology (e.g. reproductive developmental bioassays), are wide range study designs being used diversity endpoints considered. At same time, environmental hazard risk assessment, which underpins regulatory decisions protect environment from potentially harmful chemicals, often recommends that ecotoxicological data be produced following accepted validated test guidelines. These guidelines typically do not address changes, meaning these, sensitive, effects represented assessments. Here, we propose new tool, EthoCRED evaluation method, for assessing relevance reliability ecotoxicity data, considers unique requirements challenges encountered this field. This method accompanying reporting recommendations designed serve extension “Criteria Reporting Evaluating Ecotoxicity Data (CRED)” project. As such, can both accommodate array experimental design seen ecotoxicology, could readily implemented into frameworks deemed appropriate by policy makers different jurisdictions allow better integration knowledge gained testing protection. Furthermore, through our recommendations, aim improve studies peer‐reviewed literature, thereby increase their usefulness inform chemical regulation.

Language: Английский

---

Exposure Effects of Environmentally Relevant Concentrations of the Tricyclic Antidepressant Amitriptyline in Early Life Stage Zebrafish

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: July 17, 2024

Antidepressants are one of the most globally prescribed classes pharmaceuticals, and drug target conservation across phyla means that nontarget organisms may be at risk from effects exposure. Here, we address knowledge gap for chronic exposure (28 days) to tricyclic antidepressant amitriptyline (AMI) on fish, including concentrations with environmental relevance, using zebrafish (Danio rerio) as our experimental model. AMI was found bioconcentrate in zebrafish, readily transformed its major active metabolite nortriptyline, induced a pharmacological effect (downregulation gene encoding serotonin transporter; slc6a4a) environmentally relevant (0.03 μg/L above). Exposures higher accelerated hatch rate reduced locomotor activity, latter which abolished after 14 day period depuration. The lack any response features physiology behavior measured environment would indicate poses relatively low level fish populations. pseudopersistence likely presence multiple drugs acting via same mechanism action, however, together global trend increased prescription rates, mean this underestimated current ecotoxicological assessment paradigms.

Language: Английский

---

Application of a Multispecies Pulse-Exposure Microalgal Bioassay to Assess Duration and Time-of-Day Influences on the Toxicity of Chemicals

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Aquatic organisms may frequently be exposed to short-term discharges of contaminants, including those from pesticide use, stormwater runoff, or industrial effluents entering waterways. Here, a new microalgal multispecies flow cytometry-based bioassay is used assess knowledge gaps in risk assessments posed by the exposure contaminants. The toxicities atrazine, metolachlor, and copper were assessed using four scenarios, 72 h (continuous), an 18 pulse exposure, two 3 exposures (light dark conditions), that chronotoxicity. influence duration on toxicity explored utility expressions chemical-exposure dose: pulse-exposure concentration (PeC) time-weighted average concentrations (TACs). three coexisting microalgae (Monoraphidium arcuatum, Nannochloropsis-like sp., Pediastrum duplex) tolerated higher for shorter pulses compared continuous exposures. Toxicity estimates calculated TAC basis effective predicting copper. Fluorescence data collected cytometry linked physiological diel changes each species. Chronotoxicity was observed with While contaminant provide conservative estimate pulses, time are critical factors consider when assessing contaminants microalgae.

Language: Английский

---

A latitudinal gradient in the diel partitioning of species richness?

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 22, 2024

The writings of naturalists from two centuries past are brimming with accounts the stark differences in kinds and numbers organisms encountered during day night between tropical temperate zones. However, only recently have ecologists begun to systematically describe explain geographic variation diel activities species on Earth. Examining data 60 insect communities globally, I find that partitioning total richness across three activity periods tracks latitudinal gradient. In general, proportions diurnal nocturnal highest among decline poleward, while cathemeral characterises over half all at high latitudes. These trends community level broadly reflect documented patterns global distributions vertebrate using different periods. outline six hypotheses may account for a gradient richness.

Language: Английский

---

Circadian modulation of behavioural stress indicators varies between diurnal and nocturnal fish species

Applied Animal Behaviour Science, Journal Year: 2024, Volume and Issue: unknown, P. 106458 - 106458

Published: Nov. 1, 2024

Language: Английский

---