Engineering Gut Symbionts: A Way to Promote Bee Growth?
Insects,
Journal Year:
2024,
Volume and Issue:
15(5), P. 369 - 369
Published: May 19, 2024
Bees
play
a
crucial
role
as
pollinators,
contributing
significantly
to
ecosystems.
However,
the
honeybee
population
faces
challenges
such
global
warming,
pesticide
use,
and
pathogenic
microorganisms.
Promoting
bee
growth
using
several
approaches
is
therefore
for
maintaining
their
roles.
To
this
end,
bacterial
microbiota
well-known
its
native
in
supporting
respects.
Maximizing
capabilities
of
these
microorganisms
holds
theoretical
potential
promote
bees.
Recent
advancements
have
made
it
feasible
achieve
enhancement
through
application
genetic
engineering.
In
review,
we
present
roles
gut
symbionts
promoting
collectively
summarize
engineering
that
would
be
needed
future
applications.
Particularly,
has
not
been
advanced,
dominant
symbiotic
bacteria
Snodgrassella
alvi
Gilliamella
apicola
are
main
focus
paper,
along
with
other
species.
Moreover,
propose
strategies
will
allow
improvement
listed
gene
targets
modification
further
encourage
use
engineered
growth.
Language: Английский
Host specificity of gut microbiota associated with social bees: patterns and processes
Microbiology and Molecular Biology Reviews,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 20, 2025
SUMMARYGut
microbes
provide
benefits
to
some
animals,
but
their
distribution
and
effects
across
diverse
hosts
are
still
poorly
described.
There
is
accumulating
evidence
for
host
specificity
(i.e.,
a
pattern
where
different
tend
associate
with
distinct
lineages),
the
causes
consequences
of
this
unclear.
Combining
experimental
tests
in
laboratory
broad
surveys
wild
promising
approach
gaining
comprehensive
mechanistic
understanding
prevalence,
origin,
importance.
Social
bees
represent
an
ideal
testbed
endeavor
because
they
phylogenetically
functionally
diverse,
host-specific,
stable,
tractable
gut
microbiota.
Furthermore,
western
honeybee
(Apis
mellifera)
emerging
model
system
studying
microbiota-host
interactions.
In
review,
we
summarize
data
on
prevalence
strength
social
bee
microbiota
(bumblebees,
stingless
bees,
honeybees),
as
well
potential
proven
ecological
molecular
mechanisms
that
maintain
specificity.
Overall,
found
relatively
strong
likely
results
from
several
processes,
including
filtering
mediated
by
immune
priority
effects.
However,
more
research
needed
multiple
species
confirm
these
findings.
To
help
future
research,
hypotheses
field
propose
comparative
tests.
Finally,
conclude
review
highlighting
need
understand
how
can
influence
health.
Language: Английский
One-step genome engineering in bee gut bacterial symbionts
mBio,
Journal Year:
2024,
Volume and Issue:
15(9)
Published: Aug. 6, 2024
ABSTRACT
Mechanistic
understanding
of
interactions
in
many
host-microbe
systems,
including
the
honey
bee
microbiome,
is
limited
by
a
lack
easy-to-use
genome
engineering
approaches.
To
this
end,
we
demonstrate
one-step
approach
for
making
gene
deletions
and
insertions
chromosomes
gut
bacterial
symbionts.
Electroporation
linear
or
non-replicating
plasmid
DNA
containing
an
antibiotic
resistance
cassette
flanked
regions
with
homology
to
symbiont
reliably
results
chromosomal
integration.
This
lightweight
does
not
require
expressing
any
exogenous
recombination
machinery.
The
high
concentrations
large
DNAs
long
needed
make
process
efficient
can
be
readily
produced
using
modern
synthesis
assembly
methods.
We
use
knock
out
genes,
genes
involved
biofilm
formation,
insert
fluorescent
protein
into
chromosome
betaproteobacterial
Snodgrassella
alvi
.
are
also
able
engineer
genomes
multiple
strains
S.
another
species,
communis
,
which
found
bumble
microbiome.
Finally,
same
method
symbiont,
Bartonella
apis
alphaproteobacterium.
As
expected,
knockout
recA
-dependent,
suggesting
that
straightforward
procedure
applied
other
microbes
convenient
IMPORTANCE
Honey
bees
ecologically
economically
important
crop
pollinators
symbionts
influence
their
health.
Microbiome-based
strategies
studying
improving
health
have
utilized
wild-type
plasmid-engineered
bacteria.
straightforward,
single-step
used
cassettes
replace
investigating
mechanisms
community
stably
benefit
pollinator
Language: Английский
Fecal transplant allows transmission of the gut microbiota in honey bees
mSphere,
Journal Year:
2024,
Volume and Issue:
9(9)
Published: Aug. 19, 2024
ABSTRACT
The
study
of
the
fecal
microbiota
is
crucial
for
unraveling
pathways
through
which
gut
symbionts
are
acquired
and
transmitted.
While
stable
microbial
communities
essential
honey
bee
health,
their
modes
acquisition
transmission
yet
to
be
confirmed.
bees
colonized
by
symbiotic
bacteria
within
5
days
after
emergence
from
wax
cells
as
adults.
Few
studies
have
suggested
that
could
in
part
via
contact
with
matter
hive.
However,
composition
still
unknown.
It
particularly
unclear
whether
all
bacterial
species
can
found
viable
feces
therefore
transmitted
newborn
nestmates.
Using
16S
rRNA
gene
amplicon
sequencing,
we
revealed
strikingly
similar
entire
guts.
We
transplantation
resulted
those
obtained
feeding
homogenates.
Our
shows
sampling
tools
non-invasive
analysis
community
host-microbe
interactions.
also
implies
young
hive
a
plausible
route
acquisition.
IMPORTANCE
Honey
pollinators
many
crops
wildflowers.
They
powerful
models
studying
microbiome-host
current
methods
rely
on
tissue
disruption
analyze
use
homogenates
inoculate
microbiota-deprived
bees.
Here,
provide
two
new
approaches
will
open
doors
longitudinal
studies:
transplantation.
Furthermore,
our
findings
insights
into
social
insects
showing
ingestion
result
Language: Английский