MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans DOI
X. Liu,

直人 慶長,

Pingping Sun

et al.

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 224(1)

Published: Oct. 14, 2024

Maximizing cell survival under stress requires rapid and transient adjustments of RNA protein synthesis. However, capturing these dynamic changes at both single-cell level across an organism has been challenging. Here, we developed a system named MONITTR (MS2-embedded mCherry-based monitoring transcription) for real-time simultaneous measurement nascent transcripts endogenous levels in C. elegans. Utilizing this system, monitored the transcriptional bursting fasting-induced genes found that epidermis responds to fasting by modulating proportion actively transcribing nuclei kinetics individual alleles. Additionally, our findings revealed essential roles transcription factors NHR-49 HLH-30 governing fasting. Furthermore, tracked dynamics during heat-shock response ER unfolded observed conditions. Collectively, study provides foundation quantitatively investigating how animals spatiotemporally modulate various physiological pathological

Language: Английский

MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans DOI
X. Liu,

直人 慶長,

Pingping Sun

et al.

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 224(1)

Published: Oct. 14, 2024

Maximizing cell survival under stress requires rapid and transient adjustments of RNA protein synthesis. However, capturing these dynamic changes at both single-cell level across an organism has been challenging. Here, we developed a system named MONITTR (MS2-embedded mCherry-based monitoring transcription) for real-time simultaneous measurement nascent transcripts endogenous levels in C. elegans. Utilizing this system, monitored the transcriptional bursting fasting-induced genes found that epidermis responds to fasting by modulating proportion actively transcribing nuclei kinetics individual alleles. Additionally, our findings revealed essential roles transcription factors NHR-49 HLH-30 governing fasting. Furthermore, tracked dynamics during heat-shock response ER unfolded observed conditions. Collectively, study provides foundation quantitatively investigating how animals spatiotemporally modulate various physiological pathological

Language: Английский

Citations

1