Localization of Secondary Metabolites in Relict Gymnosperms of the Genus Sequoia In Vivo and in Cell Cultures In Vitro, and the Biological Activity of Their Extracts DOI Creative Commons
Rima N. Kirakosyan, Е. А. Калашникова,

Elizaveta A. Bolotina

et al.

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1694 - 1694

Published: Dec. 20, 2024

In order to scientifically search for new sources of secondary metabolites with valuable qualities phytopharmacognosy, tasks requiring a step-by-step solution were set. The primary task is the development technologies obtaining in vitro highly productive biomass cells relict gymnosperms genus Sequoia, capable accumulating various classes metabolites. study accumulation and localization allowed us evaluate biological activity cytotoxicity Sequoia cultures. our study, histochemical methods used determine compounds (phenolic terpenoid nature) plant tissues. Secondary metabolites—polyphenols, catechins, terpenoids—are mainly localized epidermal, parenchymal, conductive tissues leaves stems. callus suspension cultures cell walls vacuoles. mineral composition nutrient medium (MS WPM), light source (photoperiod), endogenous content polyphenols explant influenced initiation growth characteristics culture plants. Inhibition on WPM was noted. cultivation lines at 16 h photoperiod stimulated formation but had negative effect Extractive substances obtained from intact evergreen demonstrate high (fungicidal) cytotoxicity. inhibitory Fusarium oxisporum noted when 200 mg/L extract added medium. Extracts redwood low toxicity normal FetMSC inhibited “immortal” cervical HeLa cancer human glioblastoma A172. Intact plants initiated them are producers activity.

Language: Английский

Localization of Secondary Metabolites in Relict Gymnosperms of the Genus Sequoia In Vivo and in Cell Cultures In Vitro, and the Biological Activity of Their Extracts DOI Creative Commons
Rima N. Kirakosyan, Е. А. Калашникова,

Elizaveta A. Bolotina

et al.

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1694 - 1694

Published: Dec. 20, 2024

In order to scientifically search for new sources of secondary metabolites with valuable qualities phytopharmacognosy, tasks requiring a step-by-step solution were set. The primary task is the development technologies obtaining in vitro highly productive biomass cells relict gymnosperms genus Sequoia, capable accumulating various classes metabolites. study accumulation and localization allowed us evaluate biological activity cytotoxicity Sequoia cultures. our study, histochemical methods used determine compounds (phenolic terpenoid nature) plant tissues. Secondary metabolites—polyphenols, catechins, terpenoids—are mainly localized epidermal, parenchymal, conductive tissues leaves stems. callus suspension cultures cell walls vacuoles. mineral composition nutrient medium (MS WPM), light source (photoperiod), endogenous content polyphenols explant influenced initiation growth characteristics culture plants. Inhibition on WPM was noted. cultivation lines at 16 h photoperiod stimulated formation but had negative effect Extractive substances obtained from intact evergreen demonstrate high (fungicidal) cytotoxicity. inhibitory Fusarium oxisporum noted when 200 mg/L extract added medium. Extracts redwood low toxicity normal FetMSC inhibited “immortal” cervical HeLa cancer human glioblastoma A172. Intact plants initiated them are producers activity.

Language: Английский

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