Cul2 Is Essential for the Drosophila IMD Signaling-Mediated Antimicrobial Immune Defense

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2627 - 2627

Published: March 14, 2025

Cullin 2 (Cul2), a core component of the Cullin-RING E3 ubiquitin ligase complex, is integral to regulating distinct biological processes. However, its role in innate immune defenses remains poorly understood. In this study, we investigated functional significance Cul2 deficiency (IMD) signaling-mediated antimicrobial reactions Drosophila melanogaster (fruit fly). We demonstrated that loss-of-function led marked reduction peptide induction following bacterial infection, which was associated with increased fly mortality and load. The proteomic analysis further revealed reduced expression Effete (Eff), key E2 ubiquitin-conjugating enzyme during IMD signaling. Intriguingly, ectopic eff effectively rescued defects caused by loss Cul2. Taken together, results our study underscore critical ensuring robust signaling activation, highlighting importance defense against microbial infection Drosophila.

Language: Английский

E2 conjugating enzymes: A silent but crucial player in Ubiquitin Biology

Ageing Research Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 102740 - 102740

Published: April 1, 2025

Language: Английский