Glucokinase activity controls subpopulations of β-cells that alternately lead islet Ca2+ oscillations DOI Open Access

Erli Jin,

Jennifer K. Briggs, Richard K.P. Benninger

et al.

Published: Nov. 7, 2024

Oscillations in insulin secretion, driven by islet Ca 2+ waves, are crucial for glycemic control. Prior studies, performed with single-plane imaging, suggest that subpopulations of electrically coupled β-cells have privileged roles leading and coordinating the propagation waves. Here, we used 3D light-sheet imaging to analyze location activity single within entire at >2 Hz. In contrast network analysis indicates most highly synchronized located center, remain regionally but not cellularly stable between oscillations. This subpopulation, which includes ‘hub cells’, is insensitive changes fuel metabolism induced glucokinase pyruvate kinase activation. initiate wave (‘leaders’) periphery, strikingly, change their identity over time via rotations axis. Glucokinase activation, increased oscillation period, reinforced leader cells stabilized Pyruvate despite increasing frequency, had no effect on cells, indicating origin patterned input. These findings emphasize stochastic nature β-cell control oscillations identify a role spatially patterning ‘leader’ β-cells.

Language: Английский

Glucokinase activity controls peripherally-located subpopulations of β-cells that lead islet Ca2+ oscillations DOI Open Access

Erli Jin,

Jennifer K. Briggs, Richard K.P. Benninger

et al.

Published: Jan. 9, 2025

Oscillations in insulin secretion, driven by islet Ca 2+ waves, are crucial for glycemic control. Prior studies, performed with single-plane imaging, suggest that subpopulations of electrically coupled β-cells have privileged roles leading and coordinating the propagation waves. Here, we used 3D light- sheet imaging to analyze location activity single within entire at >2 Hz. In contrast network analysis indicates most highly synchronized located center, remain regionally but not cellularly stable between oscillations. This subpopulation, which includes ‘hub cells’, is insensitive changes fuel metabolism induced glucokinase pyruvate kinase activation. initiate wave (‘leaders’) periphery, strikingly, change their identity over time via rotations axis. Glucokinase activation, increased oscillation period, reinforced leader cells stabilized Pyruvate despite increasing frequency, had no effect on cells, indicating origin patterned input. These findings emphasize stochastic nature β-cell control oscillations identify a role spatially patterning ‘leader’ β-cells.

Language: Английский

Citations

0
Glucokinase activity controls peripherally located subpopulations of β-cells that lead islet Ca2+ oscillations DOI Creative Commons

Erli Jin,

Jennifer K. Briggs, Richard K.P. Benninger

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 12, 2025

Oscillations in insulin secretion, driven by islet Ca 2+ waves, are crucial for glycemic control. Prior studies, performed with single-plane imaging, suggest that subpopulations of electrically coupled β-cells have privileged roles leading and coordinating the propagation waves. Here, we used three-dimensional (3D) light-sheet imaging to analyze location activity single within entire at >2 Hz. In contrast 3D network analysis indicates most highly synchronized located center, remain regionally but not cellularly stable between oscillations. This subpopulation, which includes ‘hub cells’, is insensitive changes fuel metabolism induced glucokinase pyruvate kinase activation. β-Cells initiate wave (leaders) periphery, strikingly, change their identity over time via rotations axis. Glucokinase activation, increased oscillation period, reinforced leader cells stabilized Pyruvate despite increasing frequency, had no effect on cells, indicating origin patterned input. These findings emphasize stochastic nature β-cell control oscillations identify a role spatially patterning ‘leader’ β-cells.

Language: Английский

Citations

0
Glucokinase activity controls subpopulations of β-cells that alternately lead islet Ca2+oscillations DOI Creative Commons

Erli Jin,

Jennifer K. Briggs, Richard K.P. Benninger

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Abstract Oscillations in insulin secretion, driven by islet Ca 2+ waves, are crucial for glycemic control. Prior studies, performed with single-plane imaging, suggest that subpopulations of electrically coupled β-cells have privileged roles leading and coordinating the propagation waves. Here, we used 3D light- sheet imaging to analyze location activity single within entire at >2 Hz. In contrast network analysis indicates most highly synchronized located center, remain regionally but not cellularly stable between oscillations. This subpopulation, which includes ‘hub cells’, is insensitive changes fuel metabolism induced glucokinase pyruvate kinase activation. initiate wave (‘leaders’) periphery, strikingly, change their identity over time via rotations axis. Glucokinase activation, increased oscillation period, reinforced leader cells stabilized Pyruvate despite increasing frequency, had no effect on cells, indicating origin patterned input. These findings emphasize stochastic nature β-cell control oscillations identify a role spatially patterning ‘leader’ β-cells. Highlights • Studies light-sheet provide more complete picture β- cell than prior 2D studies. Highly (including cells’) regionally-stable subpopulation center metabolic perturbation. activation patterns axis, originates from periphery The stochasticity β-cells, stability ‘hubs’, geographically consistent peripheral α/δ-cells mouse islets.

Language: Английский

Citations

2
Glucokinase activity controls subpopulations of β-cells that alternately lead islet Ca2+ oscillations DOI Creative Commons

Erli Jin,

Jennifer K. Briggs, Richard K.P. Benninger

et al.

eLife, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Oscillations in insulin secretion, driven by islet Ca2+ waves, are crucial for glycemic control. Prior studies, performed with single-plane imaging, suggest that subpopulations of electrically coupled β-cells have privileged roles leading and coordinating the propagation waves. Here, we used three-dimensional (3D) light-sheet imaging to analyze location activity single within entire at >2 Hz. In contrast 3D network analysis indicates most highly synchronized located center, remain regionally but not cellularly stable between oscillations. This subpopulation, which includes 'hub cells', is insensitive changes fuel metabolism induced glucokinase pyruvate kinase activation. β-Cells initiate wave (leaders) periphery, strikingly, change their identity over time via rotations axis. Glucokinase activation, increased oscillation period, reinforced leader cells stabilized Pyruvate despite increasing frequency, had no effect on cells, indicating origin patterned input. These findings emphasize stochastic nature β-cell control oscillations identify a role spatially patterning 'leader' β-cells.

Language: Английский

Citations

1
Glucokinase activity controls subpopulations of β-cells that alternately lead islet Ca2+ oscillations DOI Open Access

Erli Jin,

Jennifer K. Briggs, Richard K.P. Benninger

et al.

Published: Nov. 7, 2024

Oscillations in insulin secretion, driven by islet Ca 2+ waves, are crucial for glycemic control. Prior studies, performed with single-plane imaging, suggest that subpopulations of electrically coupled β-cells have privileged roles leading and coordinating the propagation waves. Here, we used 3D light-sheet imaging to analyze location activity single within entire at >2 Hz. In contrast network analysis indicates most highly synchronized located center, remain regionally but not cellularly stable between oscillations. This subpopulation, which includes ‘hub cells’, is insensitive changes fuel metabolism induced glucokinase pyruvate kinase activation. initiate wave (‘leaders’) periphery, strikingly, change their identity over time via rotations axis. Glucokinase activation, increased oscillation period, reinforced leader cells stabilized Pyruvate despite increasing frequency, had no effect on cells, indicating origin patterned input. These findings emphasize stochastic nature β-cell control oscillations identify a role spatially patterning ‘leader’ β-cells.

Language: Английский

Citations

0