Statement on the active substance acetamiprid

EFSA Journal, Journal Year: 2022, Volume and Issue: 20(1)

Published: Jan. 1, 2022

Acetamiprid is a pesticide active substance with insecticidal action currently under the third renewal (AIR3) of Commission implementing regulation (EU) No 844/2012. Following concerns that this may pose high risks to humans and environment, French authorities asked restrict its uses Article 69 Regulation (EC) 1107/2009. To support request, competent Authorities from France cited series literature papers investigating hazards and/or exposure environment. Consequently, EFSA PPR Panel was mandated advise on likelihood body evidence would constitute proof serious or Therefore, evaluated these studies indicating new higher environment compared previous EU assessments.A stepwise methodology designed, including: (i) initial screening; (ii) data extraction critical appraisal based principles OHAT/NTP; (iii) weight evidence, including consideration assessments; (iv) uncertainty analysis, followed, whenever relevant, by an expert knowledge elicitation process. For human health, no conclusive assessment found for genotoxicity, developmental toxicity, neurotoxicity immunotoxicity. However, due lack adequate current set, recommends conducting endocrine disrupting properties acetamiprid in line EFSA/ECHA guidance document identification disruptors. conclusive, robust birds, aquatic organisms, bees soil organisms. potential inter-species sensitivity birds towards requires further consideration.

Language: Английский

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Genome-Wide Identification and Expression Analysis of the Cytochrome P450 Gene Family in Bemisia tabaci MED and Their Roles in the Insecticide Resistance

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5899 - 5899

Published: March 21, 2023

The whitefly, Bemisia tabaci MED (Hemiptera: Aleyrodidae), is an omnivorous agricultural pest, which causes huge economic losses to agriculture and highly resistant many pesticides. overexpression of cytochrome P450 may play important role in host adaptation insecticide resistance B. MED. Therefore, the present study systematically analyzed gene family at genome-wide level understand its function Our analysis identified 58 genes MED, among 24 were novel. Phylogenetic revealed broad functional species-specific diversification P450, suggesting multiple detoxifying. Reverse transcription-real time quantitative PCR (RT-qPCR) showed that CYP4CS2, CYP4CS5, CYP4CS6, CYP4CS8, CYP6DW4, CYP6DW5, CYP6DW6, CYP6DZ8, CYP6EN1 increased significantly after two days exposure imidacloprid. Interestingly, all nine belonged CYP4 CYP6 families. A decrease expression five (CYP6DW4, CYP4CS6) via RNA interference (RNAi) resulted a significant increase mortalities whiteflies when exposed These results indicate essential imidacloprid tolerance Thus, provides basic information on will further help elucidate mechanism pest whitefly.

Language: Английский

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Multiple stressors interact to impair the performance of bumblebee Bombus terrestris colonies

Journal of Animal Ecology, Journal Year: 2020, Volume and Issue: 90(2), P. 415 - 431

Published: Oct. 21, 2020

Bumblebees are constantly exposed to a wide range of biotic and abiotic stresses which they must defend themselves against survive. Pathogens pesticides represent important stressors that influence bumblebee health, both when acting alone or in combination. To better understand we need investigate how these factors interact, yet experimental studies date generally focus on only one two stressors. The aim this study is evaluate combined effects four (the gut parasite Nosema ceranae, the neonicotinoid insecticide thiamethoxam, pyrethroid cypermethrin EBI fungicide tebuconazole) interact affect bumblebees at individual colony levels. We established seven treatment groups colonies pulse different combinations for 2 weeks under laboratory conditions. Colonies were subsequently placed field 7 effect treatments prevalence N. ceranae inoculated bumblebees, expression levels immunity detoxification-related genes, food collection, weight gain, worker male numbers, production brood reproductives. Exposure pesticide mixtures reduced collection by bumblebees. All immunity-related genes upregulated with had not been mixtures, less likely have ceranae. Combined exposure three-pesticide mixture growth, all detrimental production. produced 40%-76% fewer queens than control colonies. Our findings show frequently come into contact health performance could therefore an impact population level. These results also significant implications current practices policies risk assessment use as tested here applied simultaneously field. Understanding interactions between will be crucial improving our ability manage bee populations ensuring pollination services future.Los abejorros están constantemente expuestos una amplia gama de agentes estresantes bióticos y abióticos los que deben defenderse para sobrevivir. Los patógenos pesticidas son importantes factores influyen en la salud abejorros, tanto cuando actúan solos como combinación. Para tener un mejor conocimiento sobre debemos investigar cómo interactúan estos estresantes, pero estudios experimentales hasta fecha generalmente se centran estudiar solo uno o dos factores. El objetivo nuestro estudio es evaluar efectos combinados cuatro (el parásito intestinal el insecticida neonicotinoide tiametoxam, piretroide cipermetrina fungicida tebuconazol) afectar nivel colonia. Establecimos siete grupos tratamiento colonias expusimos diferentes combinaciones durante semanas condiciones laboratorio, posteriormente colocaron campo semanas, efecto tratamientos prevalencia inoculados, niveles expresión relacionados con inmunidad desintoxicación, recolección alimentos, aumento peso, número obreras machos, producción cría obreras, machos reinas. La exposición mezclas redujo alimentos por parte abejorros. Todos sobre-expresaron inoculados no habían estado pesticidas, al presentaron menos probabilidades combinada mezcla tres crecimiento colonia todos tuvieron perjudiciales crías. produjeron entre 40 76% reinas las control. Nuestros hallazgos muestran entran frecuentemente contacto tiene rendimiento y, lo tanto, podría impacto poblacional. Estos resultados también tienen implicaciones prácticas políticas actuales evaluación riesgos uso plaguicidas, ya probadas aquí aplican frecuencia simultáneamente campo. Comprender interacciones estrés fundamental mejorar nuestra capacidad gestión poblaciones abejas así garantizar servicios polinización futuro.

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The neonicotinoid thiacloprid causes transcriptional alteration of genes associated with mitochondria at environmental concentrations in honey bees

Environmental Pollution, Journal Year: 2020, Volume and Issue: 266, P. 115297 - 115297

Published: Aug. 11, 2020

Thiacloprid is widely used in agriculture and may affect pollinators. However, its molecular effects are poorly known. Here, we report the global gene expression profile brain of honey bee foragers assessed by RNA-sequencing. Bees were exposed for 72 h to nominal concentrations 25 250 ng/bee via sucrose solution. Determined residue LC-MS/MS 0.59 5.49 ng/bee, respectively. exposure led 5 71 differentially expressed genes (DEGs), Nuclear encoding mitochondrial ribosomal proteins enzymes involved oxidative phosphorylation, as well metabolism transporters altered at ng/bee. Kyoto Encylopedia Genes Genomes (KEGG) analysis revealed that ribosome proteins, pyrimidine, nicotinate nicotinamide additional metabolic pathways altered. Among 21 RT-qPCR, transcript farnesol dehydrogenase juvenile hormone III synthesis was significantly down-regulated. Transcripts cyp6a14-like apolipophorin-II like protein, cytochrome oxidase (cox17) non-coding RNA (LOC102654625) up-regulated Our findings indicate thiacloprid causes transcriptional changes prominently associated with mitochondria, particularly phosphorylation. This highlight potential this neonicotinoid on energy metabolism, which compromise foraging thriving populations environmentally relevant concentrations.

Language: Английский

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Hymenoptera Genome Database: new genomes and annotation datasets for improved go enrichment and orthologue analyses

Nucleic Acids Research, Journal Year: 2021, Volume and Issue: 50(D1), P. D1032 - D1039

Published: Oct. 13, 2021

Abstract We report an update of the Hymenoptera Genome Database (HGD; http://HymenopteraGenome.org), a genomic database hymenopteran insect species. The number species represented in HGD has nearly tripled, with fifty-eight species, including twenty bees, twenty-three ants, eleven wasps and four sawflies. With reorganized website, continues to provide HymenopteraMine data mining warehouse JBrowse/Apollo genome browsers integrated BLAST. have computed Gene Ontology (GO) annotations for all greatly enhancing GO annotation gathered from UniProt more than ten-fold increase GO-annotated genes. also generated orthology datasets that encompass orthologue clusters fourteen taxonomic groups. new are available searching HymenopteraMine, as bulk file downloads.

Language: Английский

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