Cited by Drosophila ß<sub>Heavy</sub>-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil

Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil DOI

Nicole Pogodalla,

Holger Kranenburg,

Simone Rey

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Nov. 4, 2021

Abstract In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in Drosophila CNS where synapses and dendrites clustered neuropil regions. The separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute formation of an internal diffusion barrier. We find that glia polarized with a basolateral plasma membrane rich phosphatidylinositol-(3,4,5)-triphosphate (PIP 3 ) Na + /K -ATPase Nervana2 (Nrv2) abuts extracellular matrix formed at neuropil-cortex interface. apical facing phosphatidylinositol-(4,5)-bisphosphate 2 supported sub-membranous ß Heavy -Spectrin cytoskeleton. -spectrin mutant larvae affect glial polarity delocalized PIP Nrv2 exhibit abnormal locomotion similarly shown ablated larvae. Thus, compartmentalizes brain essential for proper function.

Language: Английский

Citations

A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster DOI

Emily L. Rivard,

Andrew G. Ludwig,

Prajal H. Patel

et al.

PLoS Genetics, Journal Year: 2021, Volume and Issue: 17(9), P. e1009787 - e1009787

Published: Sept. 3, 2021

Comparative genomics has enabled the identification of genes that potentially evolved de novo from non-coding sequences. Many such are expressed in male reproductive tissues, but their functions remain poorly understood. To address this, we conducted a functional genetic screen over 40 putative with testis-enriched expression Drosophila melanogaster and identified one gene, atlas , required for fertility. Detailed cytological analyses showed is proper chromatin condensation during final stages spermatogenesis. Atlas protein spermatid nuclei facilitates transition histone- to protamine-based packaging. Complementary evolutionary revealed complex history . The protein-coding portion gene likely arose at base genus on X chromosome was unlikely be essential, as it then lost several independent lineages. Within last ~15 million years, however, moved an autosome, where fused conserved RNA non-redundant role Altogether, this study provides insight into integration novel biological processes, links between genomic innovation evolution, control fundamental developmental process, gametogenesis.

Language: Английский

Citations

Identification of candidate variants and genes associated with temperature tolerance in olive flounders by Genome-Wide Association Study (GWAS) DOI

H.M.V. Udayantha,

Sukkyoung Lee,

D.S. Liyanage

et al.

Aquaculture, Journal Year: 2023, Volume and Issue: 576, P. 739858 - 739858

Published: July 6, 2023

Language: Английский

Citations

Characterization of Seizure Induction Methods inDrosophila DOI

Jurga Mituzaite, Rasmus S. Petersen, Adam Claridge‐Chang

et al.

eNeuro, Journal Year: 2021, Volume and Issue: 8(4), P. ENEURO.0079 - 21.2021

Published: July 1, 2021

Epilepsy is one of the most common neurologic disorders. Around third patients do not respond to current medications. This lack treatment indicates a need for better understanding underlying mechanisms and, importantly, identification novel targets drug manipulation. The fruit fly Drosophila melanogaster has fast reproduction time, powerful genetics, and facilitates large sample sizes, making it strong model seizure mechanisms. To understand behavioral physiological phenotypes across major genotypes we systematically measured severity secondary at both larval adult stage. Comparison several seizure-induction methods; specifically electrical, mechanical heat induction, show that electroshock effective inducing seizures wide range seizure-prone mutants tested. Locomotion in adults larvae was found be non-predictive susceptibility. Recording activity identified motor neurons revealed variations action potential (AP) patterns, different genotypes, but these patterns did correlate with conclude, while there variation phenotypes, consistent method induction known Drosophila.

Language: Английский

Citations

The ERG1 K+ Channel and Its Role in Neuronal Health and Disease DOI

Francisco G. Sanchez-Conde, Eric N. Jiménez‐Vázquez, David S. Auerbach

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: May 3, 2022

The ERG1 potassium channel, encoded by KCNH2 , has long been associated with cardiac electrical excitability. Yet, a growing body of work suggests that mediates physiology throughout the human body, including brain. is regulator neuronal excitability, variants are diseases (e.g., epilepsy and schizophrenia), serves as potential therapeutic target for pathophysiology. This review summarizes current state-of-the-field regarding channel structure function, ERG1’s relationship to mammalian brain highlights key questions have yet be answered.

Language: Английский

Citations

Knockdown of Chronophage in the nervous system mimics features of neurodevelopmental disorders caused by BCL11A/B variants DOI

Mizuki Yamaguchi,

Man Anh Huynh, Tomohiro Chiyonobu

et al.

Experimental Cell Research, Journal Year: 2023, Volume and Issue: 433(2), P. 113827 - 113827

Published: Nov. 4, 2023

Language: Английский

Citations

A novel Drosophila model for neurodevelopmental disorders associated with Shwachman–Diamond syndrome DOI

Akari Takai, Tomohiro Chiyonobu,

Ibuki Ueoka

et al.

Neuroscience Letters, Journal Year: 2020, Volume and Issue: 739, P. 135449 - 135449

Published: Oct. 25, 2020

Language: Английский

Citations

A pleiotropic chemoreceptor facilitates the production and perception of mating pheromones DOI

Cassondra Vernier, Nicole Leitner,

Kathleen M. Zelle

et al.

iScience, Journal Year: 2022, Volume and Issue: 26(1), P. 105882 - 105882

Published: Dec. 29, 2022

Optimal mating decisions depend on the robust coupling of signal production and perception because independent changes in either could carry a fitness cost. However, since signals are often mediated by different tissues cell types, mechanisms that drive maintain their remain unknown for most animal species. Here, we show Drosophila, behavioral responses to, of, putative inhibitory pheromone co-regulated Gr8a, member Gustatory receptor gene family. Specifically, through pheromonal data, found Gr8a independently regulates males females to pheromone, as well its fat body oenocytes males. Overall, these findings provide relatively simple molecular explanation how pleiotropic receptors signaling systems at population species levels.

Language: Английский

Citations

The genetic architecture of larval aggregation behavior inDrosophila DOI

Ross M. McKinney,

Ryan Valdez,

Yehuda Ben‐Shahar

et al.

Journal of Neurogenetics, Journal Year: 2021, Volume and Issue: 35(3), P. 274 - 284

Published: Feb. 25, 2021

Many insect species exhibit basal social behaviors such as aggregation, which play important roles in their feeding and mating ecologies. However, the evolutionary, genetic, physiological mechanisms that regulate aggregation remain unknown for most species. Here, we used natural populations of Drosophila melanogaster to identify genetic architecture drives larval behavior. By using quantitative reverse approaches, have identified a complex neurogenetic network plays role regulating decision larvae feed either solitude or group. Results from single gene, RNAi-knockdown experiments show several genes represent key nodes determines level while feeding. Furthermore, non-coding variant gene CG14205, putative acyltransferase, is associated with both decreased mRNA expression increased aggregate formation, suggests it has specific inhibiting Our results identify, first time, components interact naturally occurring levels D. larvae.

Language: Английский

Citations

The environmental toxicant ziram enhances neurotransmitter release and increases neuronal excitability via the EAG family of potassium channels DOI

Jenna Harrigan,

Daisy F. Brambila,

Pratap Meera

et al.

Neurobiology of Disease, Journal Year: 2020, Volume and Issue: 143, P. 104977 - 104977

Published: June 16, 2020

Environmental toxicants have the potential to contribute pathophysiology of multiple complex diseases, but underlying mechanisms remain obscure. One such toxicant is widely used fungicide ziram, a dithiocarbamate known neurotoxic effects and increase risk Parkinson's disease. We Drosophila melanogaster as an unbiased discovery tool identify novel molecular pathways by which ziram may disrupt neuronal function. Consistent with previous results in mammalian cells, we find that increases probability synaptic vesicle release dysregulation ubiquitin signaling system. In addition, excitability. Using combination live imaging electrophysiology, excitability both aminergic glutamatergic neurons. This increased phenocopied occluded null mutant animals ether a-go-go (eag) potassium channel. A pharmacological inhibitor temperature sensitive hERG (human ether-a-go-go related gene) phenocopies only at elevated temperatures. seizure (sei), fly ortholog hERG, thus another candidate target ziram. Taken together, eag family channels emerges for mediating some toxic propose human diseases blockade sei orthologs, hERG.

Language: Английский

Citations