No evidence that proteome composition is associated with realised thermal limit and dietary niche breadth in butterflies DOI Creative Commons
Fernanda S. Caron, Zuzanna Pietras,

Arkan Eddine-Lomas

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Abstract Amino acids are the building blocks of proteins that perform essential physiological functions. Theory suggests proteome composition, amino acid frequencies across all in a genome, is associated with an organism’s optimal growth temperature, offering insights into species’ temperature limits. However, this hypothesis, based on prokaryotes, has not been tested complex multicellular eukaryotes where many strictly acquired through diet. Here, we analysed proteomes orthologous and non-orthologous genes from 35 butterfly species to test for correlations maximum observed temperatures diet breadth. Using comparative approach, found no evidence composition correlates or Our findings suggest animal likely shaped more strongly by energetic biophysical constraints rather than ecological factors.

Language: Английский

Early-adult methionine restriction reduces methionine sulfoxide and extends lifespan in Drosophila DOI Creative Commons
Hina Kosakamoto, Fumiaki Obata,

Junpei Kuraishi

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 5, 2023

Methionine restriction (MetR) extends lifespan in various organisms, but its mechanistic understanding remains incomplete. Whether MetR during a specific period of adulthood increases is not known. In Drosophila, reported to extend only when amino acid levels are low. Here, by using an exome-matched holidic medium, we show that decreasing Met 10% Drosophila with or without total levels. the first four weeks adult life robustly lifespan. young flies induces expression many longevity-related genes, including sulfoxide reductase A (MsrA), which reduces oxidatively-damaged Met. MsrA induction foxo-dependent and persists for two after cessation diet. Loss attenuates extension early-adulthood MetR. Our study highlights age-dependency organismal response nutrients suggests nutrient particular sufficient healthspan extension.

Language: Английский

Citations

14
Proteome Size Is Positively Correlated with Lifespan in Mammals but Negatively Correlated with Lifespan in Birds DOI Creative Commons
Juliano Morimoto, Zuzanna Pietras

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract The central dogma describes the unidirectional flow of genetic information from DNA to proteins, leading an underappreciation potential for contained in proteomes (the full set proteins organism) reflect broader biological processes such as lifespan. Here, this is addressed by examining how size and composition 276 four vertebrate classes are related After accounting relationship between body weight lifespan, lifespan negatively correlated with proteome birds and, a weaker extent, fish, positively mammals. Proteome varies amongst classes, but there no evidence that any specific amino acid findings relation role dietary restriction discussed on extension raise questions about evolutionary structural forces shaping across species.

Language: Английский

Citations

0
Target of Rapamycin Drives Unequal Responses to Essential Amino Acid Depletion for Egg Laying in Drosophila Melanogaster DOI Creative Commons
André Nogueira Alves, Carla M. Sgrò, Matthew D. W. Piper

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: Feb. 16, 2022

Nutrition shapes a broad range of life-history traits, ultimately impacting animal fitness. A key fitness-related trait, female fecundity is well known to change as function diet. In particular, the availability dietary protein one main drivers egg production, and in absence essential amino acids laying declines. However, it unclear whether all have same impact on phenotypes like fecundity. Using holidic diet, we fed adult

Language: Английский

Citations

13
Differential amino acid usage leads to ubiquitous edge effect in proteomes across domains of life that can be explained by amino acid secondary structure propensities DOI Creative Commons
Juliano Morimoto, Zuzanna Pietras

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 26, 2024

Amino acids are the building blocks of proteins and enzymes which essential for life. Understanding amino acid usage offers insights into protein function molecular mechanisms underlying life histories. However, genome-wide patterns across domains remain poorly understood. Here, we analysed proteomes 5590 species four found that only a few consistently most least used. This differential results in lower diversity at frequent ranks, creating ubiquitous inverted U-shape pattern rank call an 'edge effect' effect likely stems from secondary structural constraints, not evolutionary chronology incorporation genetic code, highlighting functional rather than influences on usage. We also tested other contemporary hypotheses regarding varies life's is weakly influenced by growth temperature. Our findings reveal novel pervasive genomes with potential to help us probe deep relationships advance synthetic biology.

Language: Английский

Citations

2
Differential amino acid usage leads to ubiquitous edge effect in proteomes across domains of life that can be explained by amino acid secondary structure propensities DOI
Juliano Morimoto, Zuzanna Pietras

Published: July 12, 2024

Abstract Background Amino acids are the building blocks of proteins and enzymes, which pivotal for life on Earth. acid usage provides critical insights into functional constraints acting illuminates molecular mechanisms underpinning traits. Despite this, we have limited knowledge genome-wide signatures amino across domains life, precluding new genome proteome patterns to being discovered. Results Here, analysed proteomes 5,590 species four found that only a small subset is most least frequently used proteomes. This creates ubiquitous ‘edge effect’ diversity by rank arises from protein secondary structural constrains. edge effect was not driven evolutionary chronology acids, showing rather than constrains shape in proteome. We also tested contemporary hypotheses about similarities profiles relationship between growth temperature, that, contrary previous beliefs, varies temperature weakly contributes variance usage. Conclusion described novel pattern signature genomes, reveals how at level. can ultimately influence way probe deep relationships families tree engineer biology synthetic biology.

Language: Английский

Citations

0
No evidence that proteome composition is associated with realised thermal limit and dietary niche breadth in butterflies DOI Creative Commons
Fernanda S. Caron, Zuzanna Pietras,

Arkan Eddine-Lomas

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Abstract Amino acids are the building blocks of proteins that perform essential physiological functions. Theory suggests proteome composition, amino acid frequencies across all in a genome, is associated with an organism’s optimal growth temperature, offering insights into species’ temperature limits. However, this hypothesis, based on prokaryotes, has not been tested complex multicellular eukaryotes where many strictly acquired through diet. Here, we analysed proteomes orthologous and non-orthologous genes from 35 butterfly species to test for correlations maximum observed temperatures diet breadth. Using comparative approach, found no evidence composition correlates or Our findings suggest animal likely shaped more strongly by energetic biophysical constraints rather than ecological factors.

Language: Английский

Citations

0