Reported transgenerational responses to Pseudomonas aeruginosa in C. elegans are not robust DOI Creative Commons

D Patrick Gainey,

Andrey V. Shubin,

Craig P. Hunter

et al.

eLife, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Here we report our attempt to replicate reports of transgenerational epigenetic inheritance in Caenorhabditis elegans . Published results from multiple laboratories show that C. adults and their F1 embryos exposed the pathogen Pseudomonas aeruginosa aversion behavior a exposure-dependent increase daf-7/TGFβ reporter gene expression. However, one group persistence elevated daf-7 expression F2, F3, F4 generations. In contrast, failed consistently detect either avoidance response or beyond generation. We did confirm dsRNA transport proteins SID-1 SID-2 are required for intergenerational (F1) avoidance, but not Furthermore, reanalysis RNA seq data provides additional evidence this inherited PA14 may be mediated by small RNAs. The experimental methods well-described, source materials readily available, including samples reporting laboratory, explored variety environmental conditions likely account lab-to-lab variability. None these adjustments altered results. conclude example lacks robustness, response, daf-7p::gfp progeny, requires sid-1 sid-2 , identify candidate siRNAs target genes mediate response.

Language: Английский

Reported transgenerational responses to Pseudomonas aeruginosa in C. elegans are not robust DOI Creative Commons

D Patrick Gainey,

Andrey V. Shubin,

Craig P. Hunter

et al.

eLife, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Here we report our attempt to replicate reports of transgenerational epigenetic inheritance in Caenorhabditis elegans . Published results from multiple laboratories show that C. adults and their F1 embryos exposed the pathogen Pseudomonas aeruginosa aversion behavior a exposure-dependent increase daf-7/TGFβ reporter gene expression. However, one group persistence elevated daf-7 expression F2, F3, F4 generations. In contrast, failed consistently detect either avoidance response or beyond generation. We did confirm dsRNA transport proteins SID-1 SID-2 are required for intergenerational (F1) avoidance, but not Furthermore, reanalysis RNA seq data provides additional evidence this inherited PA14 may be mediated by small RNAs. The experimental methods well-described, source materials readily available, including samples reporting laboratory, explored variety environmental conditions likely account lab-to-lab variability. None these adjustments altered results. conclude example lacks robustness, response, daf-7p::gfp progeny, requires sid-1 sid-2 , identify candidate siRNAs target genes mediate response.

Language: Английский

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