Single-cell Atlas reveals core function of CPVL/MSR1 expressing macrophages in the prognosis of triple-negative breast cancer DOI Creative Commons

Xin’an Wang,

Lin Li, Xue Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Background Triple-negative breast cancer (TNBC) is the most aggressive subtype of cancer, with worst prognosis among all subtypes. The impact distinct cell subpopulations within tumor microenvironment (TME) on TNBC patient has yet to be clarified. Methods Utilizing single-cell RNA sequencing (scRNA-seq) integrated bulk (bulk RNA-seq), we applied Cox regression models compute hazard ratios, and cross-validated prognostic scoring using a GLMNET-based model. Cell communication analysis was used elucidate potential mechanisms CPVL MSR1. Ultimately, interference-mediated gene knockdown utilized validate specific genes polarization tumor-associated macrophages (TAMs). Results Our findings revealed that function immune cells more pivotal in prognosis, TAMs showing strongest correlation outcomes, compared other cells. Additionally, identified MSR1 as critical TAMs, expression positively correlated favorable outcomes associated poorer prognosis. Mechanistically, may contribute by inhibiting SPP1-CD44 ligand-receptor promoting CXCL9-CXCR3, C3-C3AR1 ligand-receptor, through which interact such monocytes, neutrophils, T Moreover, cytokines including IL-18, IFNγR1, CCL20, CCL2, along complement-related like TREM2 complement component CFD, participate process or regulating macrophage polarization. Furthermore, RT-PCR experiments confirmed M1-like TAM polarization, while linked M2-like Finally, significance these two also validated HER2-positive Conclusions are biomarkers for macrophage-mediated suggesting therapeutic targeting TNBC.

Language: Английский

The immune duality of osteopontin and its therapeutic implications for kidney transplantation DOI Creative Commons

Junto Leung,

Lei Qu,

Qifa Ye

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

Osteopontin (OPN) is a multifunctional glycoprotein with various structural domains that enable it to perform diverse functions in both physiological and pathological states. This review comprehensively examines OPN from multiple perspectives, including its protein structure, interactions receptors, immune cells, roles kidney diseases transplantation. explores the immunological duality of significance value as biomarker therapeutic target In cancer, typically promotes tumor evasion by suppressing system. Conversely, immune-related diseases, particularly transplantation, activates system enhancing migration activation thereby exacerbating damage. may stem different splice variants exposure, after cleavage, domains, which play distinct biological cellular interactions. Additionally, has significant impact posttransplantation on chronic disease and, highlighting importance potential target. Future research should further explore specific mechanisms transplantation improve treatment strategies enhance patient quality life.

Language: Английский

Citations

0
Single-cell RNA-seq data have prevalent blood contamination but can be rescued by Originator, a computational tool separating single-cell RNA-seq by genetic and contextual information DOI Creative Commons

Thatchayut Unjitwattana,

Qianhui Huang, Yiwen Yang

et al.

Genome biology, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 11, 2025

Abstract Single-cell RNA sequencing (scRNA-seq) data from complex human tissues have prevalent blood cell contamination during the sample preparation process. They may also comprise cells of different genetic makeups. We propose a new computational framework, Originator, which deciphers single by origin and separates immune those expected tissue-resident cells. demonstrate accuracy Originator at separating tissue as well origins, using variety artificially mixed real datasets, including pancreatic cancer placentas examples.

Language: Английский

Citations

0
Mechanistic study of Lonicerae Japonicae Flos (Caprifoliaceae) in non-small cell lung cancer prevention and treatment through integrative pharmacology, multi-machine learning, artificial intelligence, and in vitro experiments DOI Creative Commons
Can Liu,

Peng He,

Rui Qiao

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119832 - 119832

Published: April 1, 2025

Language: Английский

Citations

0
Heterogeneous immune landscapes and macrophage dynamics in primary and lung metastatic adenoid cystic carcinoma of the head and neck DOI Creative Commons
Xuelian Wang,

Tingyao Ma,

Hongfei Liu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 4, 2024

Recurrent or metastatic adenoid cystic carcinoma (ACC) of the head and neck is rare highly aggressive. Due to ineffectiveness immune checkpoint therapies, this study aims investigate tumor microenvironment primary tissues lung comprehend challenges immunotherapy. We analyzed RNA sequencing data constructed landscapes from 25 tumors 34 metastases. The were then validated by immunohistochemistry single-cell analysis. Compared adjacent normal tissues, both ACC showed low infiltration. Lung metastases had higher infiltration levels antigen presentation scores but also T cell exclusion dysfunction scores. Single-cell revealed abundant immunosuppressive tumor-associated macrophages in Patients with high M2 macrophage shorter metastasis-free survival. Primary exhibit heterogeneous microenvironments. Higher countered presence suppressive macrophages, which may limit effective anti-tumor responses.

Language: Английский

Citations

0
Single-cell Atlas reveals core function of CPVL/MSR1 expressing macrophages in the prognosis of triple-negative breast cancer DOI Creative Commons

Xin’an Wang,

Lin Li, Xue Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Background Triple-negative breast cancer (TNBC) is the most aggressive subtype of cancer, with worst prognosis among all subtypes. The impact distinct cell subpopulations within tumor microenvironment (TME) on TNBC patient has yet to be clarified. Methods Utilizing single-cell RNA sequencing (scRNA-seq) integrated bulk (bulk RNA-seq), we applied Cox regression models compute hazard ratios, and cross-validated prognostic scoring using a GLMNET-based model. Cell communication analysis was used elucidate potential mechanisms CPVL MSR1. Ultimately, interference-mediated gene knockdown utilized validate specific genes polarization tumor-associated macrophages (TAMs). Results Our findings revealed that function immune cells more pivotal in prognosis, TAMs showing strongest correlation outcomes, compared other cells. Additionally, identified MSR1 as critical TAMs, expression positively correlated favorable outcomes associated poorer prognosis. Mechanistically, may contribute by inhibiting SPP1-CD44 ligand-receptor promoting CXCL9-CXCR3, C3-C3AR1 ligand-receptor, through which interact such monocytes, neutrophils, T Moreover, cytokines including IL-18, IFNγR1, CCL20, CCL2, along complement-related like TREM2 complement component CFD, participate process or regulating macrophage polarization. Furthermore, RT-PCR experiments confirmed M1-like TAM polarization, while linked M2-like Finally, significance these two also validated HER2-positive Conclusions are biomarkers for macrophage-mediated suggesting therapeutic targeting TNBC.

Language: Английский

Citations

0