The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line DOI Creative Commons
Réda M. Zellag, Vincent Poupart, Takefumi Negishi

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(2), P. 115296 - 115296

Published: Feb. 1, 2025

Highlights•Mitotic spindles orient parallel to the tissue plane in C. elegans germ line•Spindle orientation is set up prophase and actively maintained through anaphase•Proper spindle depends upon LIN-5/NuMA dynein•Interphase centrosome position cortical dynamics predict orientationSummaryMitotic contributes organization shape by setting cell division plane. How coupled diverse architectures incompletely understood. The gonad a tube-shaped organ with cells forming circumferential monolayer around common cytoplasmic lumen. this during development unclear, as lack canonical cell-cell junctions that ensure other types. Here, we show microtubule force generator dynein its conserved regulator regulate are required for germline organization. We uncover cyclic, polarized pattern of localization predicts positioning throughout cycle, providing means align This work reveals new mechanism which oriented can be achieved maintain animal development.Graphical abstract

Language: Английский

The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line DOI Creative Commons
Réda M. Zellag, Vincent Poupart, Takefumi Negishi

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(2), P. 115296 - 115296

Published: Feb. 1, 2025

Highlights•Mitotic spindles orient parallel to the tissue plane in C. elegans germ line•Spindle orientation is set up prophase and actively maintained through anaphase•Proper spindle depends upon LIN-5/NuMA dynein•Interphase centrosome position cortical dynamics predict orientationSummaryMitotic contributes organization shape by setting cell division plane. How coupled diverse architectures incompletely understood. The gonad a tube-shaped organ with cells forming circumferential monolayer around common cytoplasmic lumen. this during development unclear, as lack canonical cell-cell junctions that ensure other types. Here, we show microtubule force generator dynein its conserved regulator regulate are required for germline organization. We uncover cyclic, polarized pattern of localization predicts positioning throughout cycle, providing means align This work reveals new mechanism which oriented can be achieved maintain animal development.Graphical abstract

Language: Английский

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