Biofilm architecture determines the dissemination of conjugative plasmids DOI Creative Commons

Sarah Djermoun,

Daniel K.H. Rode, Eva Jiménez‐Siebert

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(17)

Published: April 25, 2025

Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic resistance among bacteria. While molecular mechanisms have been extensively studied, our understanding plasmid dynamics within spatially structured bacterial communities and influence community architecture on remains limited. In this study, we use live-cell fluorescence microscopy investigate propagation broad host range RP4 conjugative in Escherichia coli populations exhibiting varying levels spatial organization. high-density, two-dimensional cell monolayers, direct tight contact between donors recipients not only necessary but also sufficient trigger transfer, ensuring optimal propagation. three-dimensional mature biofilms, emergent limits ability donor cells enter regions with high density, which hinders establishment contacts impedes biofilms. contrast, microcolonies, early-stage biofilms lower surface coverage leave open access points for later emerge as high-cell-density facilitates transfer. These findings reveal crucial role determining efficiency dissemination.

Language: Английский

Biofilm architecture determines the dissemination of conjugative plasmids DOI Creative Commons

Sarah Djermoun,

Daniel K.H. Rode, Eva Jiménez‐Siebert

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(17)

Published: April 25, 2025

Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic resistance among bacteria. While molecular mechanisms have been extensively studied, our understanding plasmid dynamics within spatially structured bacterial communities and influence community architecture on remains limited. In this study, we use live-cell fluorescence microscopy investigate propagation broad host range RP4 conjugative in Escherichia coli populations exhibiting varying levels spatial organization. high-density, two-dimensional cell monolayers, direct tight contact between donors recipients not only necessary but also sufficient trigger transfer, ensuring optimal propagation. three-dimensional mature biofilms, emergent limits ability donor cells enter regions with high density, which hinders establishment contacts impedes biofilms. contrast, microcolonies, early-stage biofilms lower surface coverage leave open access points for later emerge as high-cell-density facilitates transfer. These findings reveal crucial role determining efficiency dissemination.

Language: Английский

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