Microbiota-Mediated Modulation of Organophosphate Insecticide Toxicity by Species-Dependent Interactions with Lactobacilli in a Drosophila melanogaster Insect Model

Applied and Environmental Microbiology, Journal Year: 2018, Volume and Issue: 84(9)

Published: Feb. 21, 2018

Despite the benefits to global food supply and agricultural economies, pesticides are believed pose a threat health of both humans wildlife. Chlorpyrifos (CP), commonly used organophosphate insecticide, has poor target specificity causes acute neurotoxicity in wide range species via suppression acetylcholinesterase. This effect is exacerbated 10- 100-fold by chlorpyrifos oxon (CPO), principal metabolite CP. Since many animal-associated symbiont microorganisms known hydrolyze CP into CPO, we Drosophila melanogaster insect model investigate hypothesis that indigenous probiotic bacteria could affect metabolism toxicity. Antibiotic-treated germfree D. insects lived significantly longer than their conventionally reared counterparts when exposed 10 μM gut-derived Lactobacillus plantarum, but not Acetobacterindonesiensis, was shown metabolize Liquid chromatography tandem-mass spectrometry confirmed L. plantarum isolate preferentially metabolized CPO grown CP-spiked culture medium. Further experiments showed monoassociating with reestablish conventional-like sensitivity Interestingly, supplementation human rhamnosus GG (a strain binds does CP) increased survival CP-exposed suggests strain-specific differences may exist among lactobacilli emphasizes need for further investigation. In summary, these results suggest (i) formation gut microbiota can have biologically relevant consequences host, (ii) be beneficial reducing vivo toxicity.IMPORTANCE An understudied area research how (microorganisms living in/on an animal) affects toxic outcomes environmental pollutants such as pesticides. study focused specifically on microbial biotransformation (CP; common insecticide) affected host exposure toxicity parameters model. Our demonstrate had certain inadvertent pesticide suspected negatively impact off-target species, findings provide useful information wildlife conservation sustainability planning. Furthermore, highlight consider composition between pest future insecticide designs. More broadly, this supports use modulate microbiota-mediated xenobiotics.

Language: Английский

---

Pesticide degrading natural multidrug resistance bacterial flora

Microbial Pathogenesis, Journal Year: 2017, Volume and Issue: 114, P. 304 - 310

Published: Dec. 7, 2017

Language: Английский

---

The honey bee gut microbiota: strategies for study and characterization

Insect Molecular Biology, Journal Year: 2019, Volume and Issue: 28(4), P. 455 - 472

Published: Jan. 17, 2019

Abstract Gut microbiota research is an emerging field that improves our understanding of the ecological and functional dynamics gut environments. The honey bee a highly rewarding community to study, as bees are critical pollinators many crops for human consumption produce valuable commodities such wax. Most significantly, unique characteristics Apis mellifera habitat make it model system. This review discusses methods pipelines used in study Ap. closely related species four main purposes: identifying taxonomy, characterizing genomes (microbiome), microbiota–microbiota interactions functions microbial gut. purpose this contribution increase microbiota, facilitate microbiome general aid design future experiments growing field.

Language: Английский

---

Symbiont-Mediated Insecticide Detoxification as an Emerging Problem in Insect Pests

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: Sept. 30, 2020

Pesticide use is prevalent with applications from the backyard gardener to large-scale agriculture and combatting pests in homes industrial settings. More than $14 billion are spent annually US and, globally, pesticide budget estimated at almost $60 billion. Along this demand, selective pressure generated by sustained has become a concern leading environmental contamination, pest resistance, thus, reduced efficacy. Despite efforts improve impact reduce off-target effects, chemical pesticides relied on control failures costly. Though resistance mechanisms vary, one pattern that recently emerged symbiont-mediated detoxification within insect pests. The localization host, identity of symbiotic partner, stability associations across different systems vary. diversity insects ecological settings link phenomenon broad. In mini-review, we summarize recent trend insecticide modulated between bacteria insects, as well highlight implications for development, management strategies, bioremediation.

Language: Английский

---

The contribution of gut bacteria to insecticide resistance and the life histories of the major malaria vector Anopheles arabiensis (Diptera: Culicidae)

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: June 24, 2019

Abstract The gut microbiota of mosquitoes is a crucial determinant their fitness. As such, the biology Anopheles arabiensis , major malaria vector Southern Africa, was investigated. Two laboratory strains An. were used; SENN, an insecticide susceptible strain, and SENN-DDT, resistant strain. supplemented with either non-commensal bacteria or antibiotics via sucrose source to sterilize gut. fed broad-spectrum bactericidal antibiotic gentamicin, preferentially gram-positive (vancomycin), gram-negative (streptomycin) bacteriostatic (erythromycin), by sugar supplementation artificially-spiked blood-meal. effects on adult mosquito longevity resistance phenotype assessed. Bacteria from midgut both characterised MALDI-TOF mass spectroscopy. Bactericidal increased in SENN-DDT. Bacterial tolerance. Antibiotic decreased tolerance insecticides deltamethrin malathion. Blood-supplemented vancomycin resistance, while gentamicin streptomycin resistance. SENN showed greater bacterial diversity than dominated Gram-negative bacteria. This study suggests role for life history, that microflora play variable roles mosquitoes.

Language: Английский

---