Environmental DNA analysis as an emerging non-destructive method for plant biodiversity monitoring: a review DOI Creative Commons
Pritam Banerjee, Kathryn A. Stewart, Gobinda Dey

et al.

AoB Plants, Journal Year: 2022, Volume and Issue: 14(4)

Published: July 2, 2022

Abstract Environmental DNA (eDNA) analysis has recently transformed and modernized biodiversity monitoring. The accurate detection, to some extent quantification, of organisms (individuals/populations/communities) in environmental samples is galvanizing eDNA as a successful cost time-efficient biomonitoring technique. Currently, eDNA’s application plants remains more limited implementation scope compared animals microorganisms. This review evaluates the development eDNA-based methods for (vascular) plants, comparing its performance power detection with that traditional methods, critically evaluate advise best-practices needed innovate plant biomonitoring. Recent advancements, standardization field applications have provided enough utilize it conservation biology numerous organisms. Despite our demonstrating only 13% all studies focus on taxa date, considerable potential where invasive, endangered rare species, community-level interpretations proof-of-concept. Monitoring using were found be equal or effective than methods; however, species increased when both coupled. Additionally, studying interactions, community dynamics even effects anthropogenic pressure. elimination obstacles (e.g. lack relevant reference libraries plants) user-friendly protocols would greatly contribute comprehensive monitoring programs. particularly data-depauperate tropics groups (e.g., Bryophytes Pteridophytes). We further advocate coupling approaches, former often cheaper methodologically straightforward, while latter offers non-destructive approaches discrimination ability. Furthermore, make global platform eDNA, governmental academic-industrial collaborations are essential surveys broadly adopted implemented, rapid, cost-effective non-invasive approach.

Language: Английский

Ancient sedimentary DNA shows rapid post-glacial colonisation of Iceland followed by relatively stable vegetation until the Norse settlement (Landnám) AD 870 DOI Creative Commons
Inger Greve Alsos, Youri Lammers, Sofia E. Kjellman

et al.

Quaternary Science Reviews, Journal Year: 2021, Volume and Issue: 259, P. 106903 - 106903

Published: April 8, 2021

Understanding patterns of colonisation is important for explaining both the distribution single species and anticipating how ecosystems may respond to global warming. Insular flora be especially vulnerable because oceans represent severe dispersal barriers. Here we analyse two lake sediment cores from Iceland ancient sedimentary DNA infer Holocene vegetation development. Our lakes Torfdalsvatn Nykurvatn span last c. 12,000 cal yr BP 8600 BP, respectively. With near-centennial resolution, identified a total 191 plant taxa, with 152 taxa in record 172 Nykurvatn. The terrestrial at was initially dominated by bryophytes, arctic herbs such as Saxifraga spp. grasses. Around 10,100 massive immigration new observed, shrubs dwarf became common whereas aquatic macrophytes dominant. At Nykurvatn, dominant were all present earliest samples; more abundant this site than Torfdalsvatn. There an overall steep increase local accumulated richness regional pool until 8000 which time ¾ had arrived. period 4500-1000 witnessed appearance small number graminoids forbs that not recorded earlier samples. millennium, after human settlement island (Landnám), characterised sudden disappearance Juniperus communis, but also reappearance some high shrubs. Notable during coincides periods increased incidence sea ice, hypothesise have acted vector. Thus, although ongoing climate change might provide suitable habitat large range only found neighbouring regions today, reduction ice fact limit natural species.

Language: Английский

Citations

51
The Future of DNA Barcoding: Reflections from Early Career Researchers DOI Creative Commons
Danielle Grant, Ole Brodnicke, Ann Evankow

et al.

Diversity, Journal Year: 2021, Volume and Issue: 13(7), P. 313 - 313

Published: July 9, 2021

Over the last two decades, use of DNA barcodes has transformed our ability to identify and assess life on planet. Both strengths weaknesses method have been exemplified through thousands peer-reviewed scientific articles. Given novel sequencing approaches, currently capable generating millions reads at low cost, we reflect questions: What will future bring for barcoding? Will identification species using short, standardized fragments stand test time? We present reflected opinions early career biodiversity researchers in form a SWOT analysis discuss answers these questions.

Language: Английский

Citations

49
Sedimentary ancient DNA shows terrestrial plant richness continuously increased over the Holocene in northern Fennoscandia DOI Creative Commons
Dilli P. Rijal, Peter D. Heintzman, Youri Lammers

et al.

Science Advances, Journal Year: 2021, Volume and Issue: 7(31)

Published: July 30, 2021

SedaDNA reveals regional climate and bedrock nutrients as major drivers of terrestrial plant diversity in northern Fennoscandia.

Language: Английский

Citations

47
Pleistocene glacial and interglacial ecosystems inferred from ancient DNA analyses of permafrost sediments from Batagay megaslump, East Siberia DOI Creative Commons
Jérémy Courtin, Amedea Perfumo, Andrei Andreev

et al.

Environmental DNA, Journal Year: 2022, Volume and Issue: 4(6), P. 1265 - 1283

Published: July 8, 2022

Abstract Pronounced glacial and interglacial climate cycles characterized northern ecosystems during the Pleistocene. Our understanding of resultant community transformations past ecological interactions strongly depends on taxa found in fossil assemblages. Here, we present a shotgun metagenomic analysis sedimentary ancient DNA ( seda DNA) to infer ecosystem‐wide biotic composition (from viruses megaherbivores) from Middle Late Pleistocene at Batagay megaslump, East Siberia. The records vegetation largely agree with pollen plant metabarcoding data same samples. Interglacial attributed Marine Isotope Stage (MIS) 17 MIS 7 were by forested Pinus , Betula Alnus ) open grassland. microbial fungal communities indicate strong activity related soil decomposition, especially MIS17. local landscape likely featured more open, herb‐dominated areas, mosaic supported birds small omnivorous mammals. Parts area intermittently/partially flooded as suggested presence water‐dependent taxa. During 3, sampled are identified cold‐temperate, periodically Diverse megafauna Mammuthus Equus Coelodonta coexisted mammals (rodents). 2 existed under harsher conditions, cold‐adapted herbaceous Typical still inhabited area. new approach, which sequencing is data, enables investigation changes across broad range taxonomic groups inferences about trophic aspects ecology.

Language: Английский

Citations

32
Environmental DNA analysis as an emerging non-destructive method for plant biodiversity monitoring: a review DOI Creative Commons
Pritam Banerjee, Kathryn A. Stewart, Gobinda Dey

et al.

AoB Plants, Journal Year: 2022, Volume and Issue: 14(4)

Published: July 2, 2022

Abstract Environmental DNA (eDNA) analysis has recently transformed and modernized biodiversity monitoring. The accurate detection, to some extent quantification, of organisms (individuals/populations/communities) in environmental samples is galvanizing eDNA as a successful cost time-efficient biomonitoring technique. Currently, eDNA’s application plants remains more limited implementation scope compared animals microorganisms. This review evaluates the development eDNA-based methods for (vascular) plants, comparing its performance power detection with that traditional methods, critically evaluate advise best-practices needed innovate plant biomonitoring. Recent advancements, standardization field applications have provided enough utilize it conservation biology numerous organisms. Despite our demonstrating only 13% all studies focus on taxa date, considerable potential where invasive, endangered rare species, community-level interpretations proof-of-concept. Monitoring using were found be equal or effective than methods; however, species increased when both coupled. Additionally, studying interactions, community dynamics even effects anthropogenic pressure. elimination obstacles (e.g. lack relevant reference libraries plants) user-friendly protocols would greatly contribute comprehensive monitoring programs. particularly data-depauperate tropics groups (e.g., Bryophytes Pteridophytes). We further advocate coupling approaches, former often cheaper methodologically straightforward, while latter offers non-destructive approaches discrimination ability. Furthermore, make global platform eDNA, governmental academic-industrial collaborations are essential surveys broadly adopted implemented, rapid, cost-effective non-invasive approach.

Language: Английский

Citations

31