Beta diversity patterns and driving mechanisms of stream bacteria and fungi on Mt. Kilimanjaro DOI Creative Commons

Beryl Ochieng,

Hao Wu, Yanan Zhou

et al.

Ecological Informatics, Journal Year: 2024, Volume and Issue: 82, P. 102747 - 102747

Published: July 29, 2024

Aquatic microbes, which are highly diverse in freshwater biofilms, play a vital role shaping the structure and functioning of ecosystems. Nevertheless, influence environmental factors on aquatic microbes different biogeographic ecosystems, especially tropical mountains, is relatively unexplored. Here, we examined relative climate physicochemical beta diversity stream bacteria fungi using 16S rRNA amplicon sequencing at 40 sites Mt. Kilimanjaro. Towards larger elevational distance, total turnover increased significantly, while nestedness decreased. The showed greater spatial variation than that bacteria, local contribution to (LCBD) U-shaped pattern for both taxa. Bacteria had strong positive relationships regarding diversity, LCBD their components, except nestedness, indicating similar preferences resource overlap. Among variables, mean annual temperature, water depth elevation predicted microbial depth, temperature precipitation primarily affected LCBD. Overall, climate-related variables were dominant effects more pronounced bacteria. contrasting patterns bacterial fungal ecological interactions ecosystems provide rare dataset assessing functions understudied predicting impact global change microorganisms.

Language: Английский

Microbial functional assemblages predicted by the FAPROTAX analysis are impacted by physicochemical properties, but C, N and S cycling genes are not in mangrove soil in the Beibu Gulf, China DOI Creative Commons

Zicong Yang,

Chunyan Peng,

Hongming Cao

et al.

Ecological Indicators, Journal Year: 2022, Volume and Issue: 139, P. 108887 - 108887

Published: April 22, 2022

Mangroves and their global significance in climate change based on the storage of blue carbon release CO2, CH4, N2O have attracted great attention. Microbial communities functions are essential for biogeochemical cycle (C), nitrogen (N), sulfur (S). Therefore, assessment microbial mangrove habitats, which suffer from different external environments, is necessary to evaluate unique ecological service functions. In present work, functional profiles four reserves (one sandy three muddy, Beibu Gulf, China) were detected by 16 s RNA gene amplicon-based Functional Annotation Procaryotic Taxa (FAPROTAX) analysis as well C, N, S q-PCR chips. The FAPROTAX showed that metabolic potential cycling indicated a difference regions. Moreover, some groups involved significantly correlated with physicochemical characteristics. However, genes chip did not same changes following variation environmental conditions those predicted analysis. Most equally distributed mangroves. Several N (amoA, amoB, nirK, nosZ), degradation (amyA, apu, mnp, lig, chiA), fixation (aclB), oxidation H2S (SoxY) higher sampling site Beihai City (Bei), was only soils. We hypothesized soil texture (sandy or muddy) rather than elements contributes more abundance S, C sediment. Our study demonstrated property easier modify genes. should pay attention redundancy microbiota when using microorganisms indicator access activity habitats.

Language: Английский

Citations

54

Analysis and Interpretation of metagenomics data: an approach DOI Creative Commons

Gauri S. Navgire,

Neha Goel, Gifty Sawhney

et al.

Biological Procedures Online, Journal Year: 2022, Volume and Issue: 24(1)

Published: Nov. 19, 2022

Abstract Advances in next-generation sequencing technologies have accelerated the momentum of metagenomic studies, which is increasing yearly. The metagenomics field one versatile applications microbiology, where any interaction environment involving microorganisms can be topic study. Due to this versatility, number omics technology reached its horizons. Agriculture a crucial sector crop plants and interacting together. Hence, studying these interactions through lenses would completely disclose new meaning health development. rhizosphere an essential reservoir microbial community for agricultural soil. we focus on R&D studies crops such as rice, wheat, legumes, chickpea, sorghum. These recent developments are impossible without continuous advancement seen platforms; thus, brief introduction analysis available platforms presented here clear picture workflow. Concluding discussion about different pipelines applied analyze data produced by techniques significant role interpreting outcome particular experiment. A plethora software tools incorporated automated or individually perform manual analysis. Here describe 8–10 advanced, efficient used that explain their respective workflows simplify whole process.

Language: Английский

Citations

44

Zonation of bulk and rhizosphere soil bacterial communities and their covariation patterns along the elevation gradient in riparian zones of three Gorges reservoir, China DOI

Weitao Jia,

Ping Huang, Kai Zhu

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 249, P. 118383 - 118383

Published: Feb. 7, 2024

Language: Английский

Citations

6

Spartina alterniflora invasion and mangrove restoration alter diversity and composition of sediment diazotrophic community DOI
Xiaofang Huang, Jianxiang Feng, Junde Dong

et al.

Applied Soil Ecology, Journal Year: 2022, Volume and Issue: 177, P. 104519 - 104519

Published: May 7, 2022

Language: Английский

Citations

23

Anthropogenic activities and geographic locations regulate microbial diversity, community assembly and species sorting in Canadian and Indian freshwater lakes DOI
Chinedu C. Obieze, Gowher A. Wani, Manzoor A. Shah

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 826, P. 154292 - 154292

Published: March 4, 2022

Language: Английский

Citations

20

A Tight Interaction between the Native Seagrass Cymodocea nodosa and the Exotic Halophila stipulacea in the Aegean Sea Highlights Seagrass Holobiont Variations DOI Creative Commons
Chiara Conte, Eugenia T. Apostolaki, Salvatrice Vizzini

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(2), P. 350 - 350

Published: Jan. 11, 2023

Seagrasses harbour bacterial communities with which they constitute a functional unit called holobiont that responds as whole to environmental changes. Epiphytic rapidly respond both biotic and abiotic factors, potentially contributing the host fitness. The Lessepsian migrant Halophila stipulacea has high phenotypical plasticity harbours highly diverse epiphytic community, could support its invasiveness in Mediterranean Sea. current study aimed evaluate Halophila/Cymodocea competition Aegean Sea by analysing each of two seagrasses meadow zone where these intermingled, well their monospecific zones, at depths. Differences holobionts were evaluated using seagrass descriptors (morphometric, biochemical, elemental, isotopic composition) assess changes, 16S rRNA gene identify community structure composition. An Indicator Species Index was used bacteria significantly associated host. In mixed meadows, native C. nodosa shown be affected presence exotic H. stipulacea, terms plant communities, while responded only factors rather than proximity. This provided evidence competitive advantage on suggests possible use an ecological descriptor.

Language: Английский

Citations

12

Assembly processes underlying bacterial community differentiation among geographically close mangrove forests DOI Creative Commons
Lu Liu, Nan Wang, Min Liu

et al.

mLife, Journal Year: 2023, Volume and Issue: 2(1), P. 73 - 88

Published: March 1, 2023

Abstract Bacterial communities play pivotal roles in nutrient cycling mangrove forests. The assembly of microbial has been found to be influenced by complex factors, such as geographic distance, physicochemical conditions, and plant identity, but the relative importance these factors how shape assembling process remain elusive. We analyzed bacterial sampled from three species ( Aegiceras corniculatum , Bruguiera sexangula Kandelia obovata ) at locations along estuarine Dongzhai Harbor Hainan, China. revealed larger differences rhizosphere among geographical than species, indicated diversity, composition, interaction networks. that dispersal limitation homogeneous selection have substantial contributions all locations. Following phylogenetic‐bin‐based null model analysis (iCAMP) framework, we also showing dominance some bins. greater may mainly attributed proportions even a short distance. beta diversity was positively correlated with environmental distances, implying more similar conditions (such rich carbon nitrogen contents) shaped communities. concluded which are associated limitation, played key role communities, while identity contributed less.

Language: Английский

Citations

12

Chemoautotrophic sulphur oxidizers dominate microbial necromass carbon formation in coastal blue carbon ecosystems DOI
Xiaoli Yu, Lu Qian, Qichao Tu

et al.

Functional Ecology, Journal Year: 2023, Volume and Issue: 37(10), P. 2634 - 2651

Published: Aug. 24, 2023

Abstract Coastal blue carbon (C) ecosystems are recognized as efficient natural C sinks and play key roles in mitigating global climate change. Microbially driven C, nitrogen (N) sulphur (S) cycles crucial for ecosystem functioning, but how microorganisms drive sink formation sequestration coastal sediments remains unclear. In this study, we conducted a comprehensive analysis of amino sugars, N S cycling genes/pathways their associated taxa native ( Cyperus malaccensis Kandelia obovata ) alien Spartina alterniflora Sonneratia apetala vegetation. Compared to the alien‐vegetated sediment, native‐vegetated sediment had significantly p < 0.05) higher microbial necromass functional potentials chemoautotrophic fixation, degradation, methane cycling, 2 oxidation sulphate reduction. Also, our microbiomes showed that could be coupled with fixation and/or nitrate/nitrite oxidation, degradation were found pathways predicting C. Additionally, sulphur‐oxidizing Burkholderiales metagenome‐assembled genomes (MAGs) group dominated sediments. These results suggested oxidizers, particular novel lineage, might dominates through anabolism (C fixation);the coupling microbially processes; deposition derived This study provides insights into importance oxidizers shed new light on mechanism ecosystems, which also has important implications enhancing wetlands. Read free Plain Language Summary article Journal blog.

Language: Английский

Citations

11

Uncovering potential mangrove microbial bioindicators to assess urban and agricultural pressures on Martinique island in the eastern Caribbean Sea DOI Creative Commons

Maud Fiard,

Cécile Militon,

Léa Sylvi

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 928, P. 172217 - 172217

Published: April 6, 2024

Language: Английский

Citations

4

Evaluation of soil fungal communities using the ITS2 sublocus and 18S gene primers under different amplification methods DOI
Godar Sene, Chinedu C. Obieze, Obinna T. Ezeokoli

et al.

Fungal ecology, Journal Year: 2025, Volume and Issue: 76, P. 101425 - 101425

Published: April 10, 2025

Language: Английский

Citations

0