A guide to current methodology and usage of reverse vaccinology towardsin silicovaccine discovery

FEMS Microbiology Reviews, Journal Year: 2023, Volume and Issue: 47(2)

Published: Feb. 16, 2023

Reverse vaccinology (RV) was described at its inception in 2000 as an silico process that starts from the genomic sequence of pathogen and ends with a list potential protein and/or peptide candidates to be experimentally validated for vaccine development. Twenty-two years later, this has evolved few steps entailing handful bioinformatics tools multitude plethora tools. Other related processes overlapping workflow have also emerged terms such subtractive proteomics, computational vaccinology, immunoinformatics. From perspective new RV practitioner, determining appropriate can time consuming overwhelming task, given number choices. This review presents current understanding usage research community determined by comprehensive survey scientific papers published last seven years. We believe mainstream presented here will valuable guideline all researchers wanting apply up-to-date discovery process.

Language: Английский

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High-Resolution Linear Epitope Mapping of the Receptor Binding Domain of SARS-CoV-2 Spike Protein in COVID-19 mRNA Vaccine Recipients

Microbiology Spectrum, Journal Year: 2021, Volume and Issue: 9(3)

Published: Nov. 10, 2021

Establishing vaccine-based population immunity has been the key factor in attaining herd protection. Thanks to expedited worldwide research efforts, potency of mRNA vaccines against coronavirus disease 2019 (COVID-19) is now incontestable.

Language: Английский

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Differential patterns of cross-reactive antibody response against SARS-CoV-2 spike protein detected for chronically ill and healthy COVID-19 naïve individuals

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Oct. 7, 2022

Immunity to previously encountered viruses can alter response unrelated pathogens. We reasoned that similar mechanism may also involve SARS-CoV-2 and thereby affect the specificity quality of immune against virus. Here, we employed high-throughput next generation phage display method explore link between antibody antigens spike (S) glycoprotein. By profiling in COVID-19 naïve individuals with a diverse clinical history (including cardiovascular, neurological, or oncological diseases), identified 15 highly antigenic epitopes on protein showed cross-reactivity seasonal, persistent, latent chronic infections from common human viruses. observed varying degrees different viral S an epitope-specific manner. The data show pre-existing S1 S2 cross-reactive serum is readily detectable pre-pandemic cohort. In severe cases, found differential defined spike. noted despite high mutation rates Omicron (B.1.1.529) variants SARS-CoV-2, some overlapped described mutations. Finally, propose resolved if targeted by re-called vaccinations function chronically ill naïve/unvaccinated as immunogenic targets boost antibodies augmenting conditions. Understanding relationships prior antigen exposure at epitope level subsequent strain paramount guiding strategies exit pandemic.

Language: Английский

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Evaluation of a Tilapia Vaccine Against Streptococcus Agalactiae Epitopes Based on Phage Display Technology

Published: Jan. 1, 2025

Language: Английский

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Longitudinal Variations in Antibody Responses against SARS-CoV-2 Spike Epitopes upon Serial Vaccinations

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7292 - 7292

Published: April 14, 2023

The COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) impacted healthcare, workforce, and worldwide socioeconomics. Multi-dose mono- or bivalent mRNA vaccine regimens have shown high efficacy in protection against SARS-CoV-2 its emerging variants with varying degrees of efficacy. Amino acid changes, primarily receptor-binding domain (RBD), result selection for viral infectivity, disease severity, immune evasion. Therefore, many studies centered around neutralizing antibodies that target RBD their generation achieved through infection vaccination. Here, we conducted a unique longitudinal study, analyzing effects three-dose regimen exclusively using monovalent BNT162b2 (Pfizer/BioNTech) vaccine, systematically administered to nine previously uninfected (naïve) individuals. We compare changes humoral antibody responses across entire spike glycoprotein (S) high-throughput phage display technique (VirScan). Our data demonstrate two doses vaccination alone can achieve broadest highest magnitudes anti-S response. Moreover, present evidence novel highly boosted non-RBD epitopes strongly correlate neutralization recapitulate independent findings. These vaccine-boosted could facilitate multi-valent development drug discovery.

Language: Английский

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Characterization and Utilization of Disulfide-Bonded SARS-CoV-2 Receptor Binding Domain of Spike Protein Synthesized by Wheat Germ Cell-Free Production System

Viruses, Journal Year: 2022, Volume and Issue: 14(7), P. 1461 - 1461

Published: July 1, 2022

The spike protein (SP) of SARS-CoV-2 is an important target for COVID-19 therapeutics and vaccines as it binds to the ACE2 receptor enables viral infection. Rapid production functional characterization properly folded SP utmost importance studying immunogenicity receptor-binding activity this considering emergence highly infectious variants. In study, we attempted express region (RBD) containing disulfide bonds using wheat germ cell-free synthesis system. By adding isomerase (PDI) endoplasmic reticulum oxidase (ERO1α) translational reaction mixture, succeeded in synthesizing a functionally intact RBD that can interact with ACE2. Using protein, have developed high-throughput AlphaScreen assay evaluate RBD–ACE2 interaction, which be applied drug screening mutation analysis. Thus, our method sheds new light on structural properties has potential contribute development therapeutics.

Language: Английский

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Evaluation of epitope vaccine based on phage display technology for largemouth bass virus

Aquaculture, Journal Year: 2023, Volume and Issue: 580, P. 740288 - 740288

Published: Oct. 29, 2023

Language: Английский

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Comparative analysis of eleven SARS-CoV-2 immunoassays and neutralisation data: time to enhance standardisation and correlation of protection

Infectious Diseases, Journal Year: 2024, Volume and Issue: 56(12), P. 1067 - 1079

Published: July 24, 2024

To infer a reliable SARS-CoV-2 antibody protection level from serological test, an appropriate quantitative threshold and solid equivalence across tests are needed. Additionally, should show correlation with neutralising assays the observed in large population cohorts even against emerging variants.

Language: Английский

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Application of Organoids in Carcinogenesis Modeling and Tumor Vaccination

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: March 17, 2022

Organoids well recapitulate organ-specific functions from their tissue of origin and remain fundamental aspects organogenesis. are widely applied in biomedical research, drug discovery, regenerative medicine. There various cultivated organoid systems induced by adult stem cells pluripotent cells, or directly derived primary tissues. Researchers have drawn inspiration combination technology engineering to produce organoids with more physiological relevance suitable for translational This review describes the value applying tumorigenesis modeling tumor vaccination. We summarize application precision Extant challenges that need be conquered make this feasible precise discussed.

Language: Английский

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Modular nanoarray vaccine for SARS-CoV-2

Nanomedicine Nanotechnology Biology and Medicine, Journal Year: 2022, Volume and Issue: 46, P. 102604 - 102604

Published: Sept. 13, 2022

Language: Английский

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