Effect of silencing Thrips palmi Btk29A and COL3A1 on fitness and virus acquisition

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 19, 2023

Thrips palmi (Thysanoptera: Thripidae) is a major agricultural pest infesting over 200 plant species. Along with direct injury caused by feeding, T. spreads several orthotospoviruses. Groundnut bud necrosis orthotospovirus (GBNV, family Tospoviridae , genus Orthotospovirus ) the predominant in Asia, vectored . It responsible for almost 89 million USD losses Asia annually. Several transcripts of related to innate immune response, receptor binding, cell signaling, cellular trafficking, viral replication, and apoptosis are responsive infection orthotospoviruses thrips. Expression tyrosine kinase Btk29A isoform X1 ( collagen alpha-1(III) chain-like COL3A1 significantly regulated post-GBNV capsicum chlorosis infection. In present study, were silenced effect on virus titer fitness was assessed. The expression reduced 3.62 3.15-fold, respectively, 24 h post-dsRNA exposure. Oral administration dsRNAs induced 60 50.9% mortality GBNV concentration dropped post-silencing contrast, silencing led an increase compared untreated control. To best our knowledge, this first report palmi.

Language: Английский

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Why Sound? Diversity and Evolution of Sonic Defences in Caterpillars

Published: Jan. 1, 2024

Defensive sound production is widespread and highly diverse among insects, yet the specific functions factors affecting its evolution are poorly understood. In this thesis, I have two overarching objectives. 1) conduct a literature review on insect defence sounds show that been reported in 12 orders with an enormous variety of sound-producing mechanisms, acoustic features, proposed functions. Based my review, pose hypotheses predictions to explain diversity sonic defences. 2) study caterpillars Superfamily Bombycoidea (silk hawk moths) test some these using experimental phylogenetic comparative methods. do three stages. First, document defensive responses 44 species (33 previously untested), bringing total number tested our database 97. 34/97 produce sounds. Second, characterize different mechanisms (clicking, chirping, whistling, vocalizing), contributing new data for 13 unreported producers. Third, use information develop how evolved function by mapping onto phylogeny constructed. Interestingly, occur multiple distantly related groups, suggesting convergent evolution; yet, closely can differ their abilities or mechanisms. These results suggest there important selective pressures evolving sounds, could vary types Using methods, explore possible "why sound". Two stand out: body size (based head capsules) trade-offs secretions. also found support hypothesis short (i.e., clicking chirping) aposematic because they correlate presence chemical Overall, research offers valuable contributions sounds: provide first comprehensive topic, establish model system characterizing defences (and other responses) caterpillars, key evolutionary origins, pressures,

Language: Английский

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Streamlined boiling lysis DNA extraction for Gram-positive aquaculture pathogen Streptococcus agalactiae

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(11)

Published: Oct. 17, 2024

Language: Английский

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Rapid and Accurate Detection of Chrysomya megacephala (Diptera: Calliphoridae) Using Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 1008 - 1008

Published: Dec. 20, 2024

Estimating the postmortem interval (PMI) is critical in field of forensic science, and necrophagous insects play a significant role this process. Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) common insect species, making its rapid accurate identification essential. However, commonly used molecular biology methods, such as DNA barcode, still have some limitations identifying they are often complex, time-consuming, reliant on laboratory instruments. Therefore, study, we developed an innovative detection system for C. based Cytochrome b gene using recombinase polymerase amplification (RPA) lateral flow dipstick (LFD) combination. The RPA-LFD achieved complete just 15 min at 37 °C with good sensitivity specificity. Only 7.8 × 10−4 ng or more target fragments were required, positive rate 100% was 18 samples from actual cases. In addition, ability combination extraction methods to enable on-site preliminarily explored. results suggested that when combined grinding ddH2O method (a method), process species acquisition visualization could be completed less than 20 min. conclusion, outperforms terms speed, convenience, it suitable direct application crime scenes, promising provide important assistance estimating PMI expanding impact entomological evidence.

Language: Английский

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Evaluation of Protocols for DNA Extraction from Individual Culex pipiens to Assess Pyrethroid Resistance Using Genotyping Real-Time Polymerase Chain Reaction

Methods and Protocols, Journal Year: 2024, Volume and Issue: 7(6), P. 106 - 106

Published: Dec. 23, 2024

Culex pipiens is a major vector of pathogens, including West Nile and Usutu viruses, that poses significant public health risk. Monitoring pyrethroid resistance in mosquito populations essential for effective control. This study aims to evaluate four DNA extraction protocols—QIAsymphony, DNAzol® Direct reagent, PrepMan® Ultra Sample Preparation Reagent (USPR), Chelex® 100—to identify an optimal method extract from individual pipiens, as part high-throughput surveillance using Real-Time Genotyping PCR. The target the L1014F mutation voltage-sensitive sodium channel (VSSC) gene, which confers knockdown (kdr) pyrethroids. Mosquitoes were collected wintering summer habitats Lazio Tuscany, Italy, was extracted methods. quality, quantity, time, cost compared among various USPR protocol most efficient, providing high-quality with 260/280 purity ratio within range at lowest short time. also demonstrated highest amplification success rate (77%) subsequent real-time PCR assays, making it preferred large-scale genotyping studies.

Language: Английский

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Linear Vector Quantization for the Diagnosis of Ground Bud Necrosis Virus in Tomato

International Journal of Electrical and Electronics Research, Journal Year: 2022, Volume and Issue: 10(4), P. 906 - 914

Published: Dec. 3, 2022

In this varying environment, a correct and appropriate disease diagnosis including early preclusion has never been more significant. Our study on identification of groundnut originated by Groundnut Bud Necrosis Virus will cover the way to effective use image processing approach in agriculture. The difficulty capable plant protection is very much linked problems sustainable agriculture climate change. Due fast advancement Artificial Intelligence, work paper primarily focused applying Pattern Recognition based techniques. purpose determine grade control developing model for selection bud blight caused GBNV tomatoes. images are classified according disease. Different methods have applied make proper bringing clarity diagnostic results. Linear Vector Quantization works well than, Radial Basis Function, Back Propagation Neural Network Support Machine.

Language: Английский

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Rapid assay using recombinase polymerase amplification and lateral flow dipstick for identifying Agrilus mali, a serious wood-boring beetle of the western Tianshan Mountains in China

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: April 3, 2023

Abstract Agrilus mali is a major wood-boring pest of Northeast Asia. Recently, it destroyed Malus sieversii and caused the decline wild fruit forests Tianshan Mountains in Xinjiang Uygur Autonomous Region, China. The beetle often not identified due to absence affective rapid discrimination methods beyond adult morphological examination. This study reports simple, rapid, specific sensitive method based on recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD), which able quickly define even when young. RPA primers were designed internal transcribed spacer 1 region (ITS1). introduction probe reaction eliminated false-positive signals. (Probe1-b) was specificity sensitivity as little 10 -3 ng purified DNA needed detect A. . Moreover, carried out using crude extracted DNA, only by immersion sterile distilled water. LFD used visualize detection results. results show that whole process RPA-LFD assay can be completed at 38 °C approximately 30 min, including min for gDNA extraction 15 detection, an individual’s hand; thus, this has very good application prospects field quarantine management.

Language: Английский

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Genomic Characterization of Endosymbiotic Bacteria Associated With Helicoverpa armigera in Iran Using Next-Generation Sequencing

Bioinformatics and Biology Insights, Journal Year: 2023, Volume and Issue: 17

Published: Jan. 1, 2023

Several species of the Helicoverpa genus have been recognized as major agricultural pests from different regions world, among which armigera has reported most destructive and cosmopolitan in including Iran. This pest is a polyphagous can cause damage to more than 120 plant species. Studying internal microbiome very important identifying species' weaknesses natural enemies potential biological control agents. For genomic characterization microbial community associated with H armigera, whole genome insect larvae collected vegetable fields northwest Iran was sequenced using next-generation sequencing Illumina platform. Finally, about 2 GB raw data were obtained. Using MetaPhlAn2 pipeline, it predicted that endosymbiont bacterial Buchnera aphidicola Serratia symbiotica armigera. Alignment reference strains sequences related both endosymbiotic bacteria subsequently, assembly analyses resulted genomes 657 623 bp length GC content 27.4% for B 1 595 135 42.90% S symbiotica. research first report on association worldwide.

Language: Английский

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