Ultrasound-mediated drug diffusion, uptake, and cytotoxicity in glioblastoma 3D tumour sphere model DOI Creative Commons
Janith Wanigasekara, Julie Rose Mae Mondala, Patrick J. Cullen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 27, 2023

Abstract A myriad of biological effects can be stimulated by the ultrasound (US) for treatment cancer. The objective our research was to investigate effect alone and in combination with chemotherapeutic drugs such as doxorubicin (DOX) temozolomide (TMZ) on human glioblastoma (GBM) epidermoid carcinoma cancer 2D 3D cell cultures. Results indicated that US 96-probe device could induce tumour sphere cytotoxicity a dosage- time-dependent manner, multiple treatments augmenting this cytotoxic effect. With enhanced cytotoxicity, decreased growth metabolic activity, disrupted spheroid integrity, heightened occurrence DNA double strand breaks, resulting damage spheres an inability rebuild after treatments. TMZ / DOX efficiency GBM enhancing induced compared monolayer cells, also increasing incubation time, which is most crucial way differentiate between effectiveness drug without US. In conclusion, data demonstrate enhances diffusion, uptake, using models when It demonstrates significance culture delivery discovery research. Graphical

Language: Английский

Recent progress on the organoids: Techniques, advantages and applications DOI
Xiaofeng Liu, Zhiyuan Zhou, Yao Zhang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 185, P. 117942 - 117942

Published: March 4, 2025

Language: Английский

Citations

1
Ultrasensitive Dual Fluorophore-Conjugated Carbon Dots for Intracellular pH Sensing in 3D Tumor Models DOI
Nian Kee Tan,

Hazel Chan,

Zufu Lu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(36), P. 47303 - 47313

Published: Aug. 31, 2024

The dysregulation of pH has been linked to the onset chronic conditions, such as cancer and neurological diseases. Consequently, development a highly sensitive tool for intracellular sensing is imperative investigate interplay between biochemical changes accompanying disease pathogenesis. Here, we present ratiometric fluorescent nanoprobe, NpRhoDot, designed precisely measuring levels. We demonstrate its efficacy in sensitively reporting monolayer A549 lung cells, primary fibroblast 3D tumor spheroids derived from DLD-1 colorectal adenocarcinoma cell line. NpRhoDot leverages novel design, where stable carbon dots are functionalized with pH-responsive probe comprising naphthalimide-rhodamine moiety, NpRho1. This design confers high sensitivity characteristics organic probes, along excellent photostability up 1 h biocompatibility dots. Through one-photon two-photon fluorescence microscopy, validate reliability biosensing three-dimensional models 4 7.

Language: Английский

Citations

8
3D tumor spheroids: morphological alterations a yardstick to anti-cancer drug response DOI Creative Commons
Anna Senrung, Sakshi Lalwani, Divya Janjua

et al.

In vitro models, Journal Year: 2023, Volume and Issue: 2(6), P. 219 - 248

Published: Sept. 20, 2023

Tumor spheroids are one of the well-characterized 3D culture systems bearing close resemblance to physiological tissue organization and complexity avascular solid tumor stage with hypoxic core. They hold a wide-spread application in field pharmaceutical science anti-cancer drug research. However, difficulty determining optimal technique for generation uniform size shape, evaluation experimental outputs, or mass production often limits their usage research high-throughput screening. In recent times, several studies have demonstrated various simple techniques generating uniform-size spheroids, including hanging drop (HD), liquid overlay (LOT), microfluidic approaches. Morphological alterations apart from biochemical assays, staining suitably employed outcomes within spheroid models. response effective treatment such as reduced size, loss compactness integrity smooth surface, highly reliable. These can significantly reduce need assays techniques, resulting both time cost savings. The present article specifically covers variety available procedures generation. For practical applicability, we supplemented our review study glioblastoma U87 using HD LOT methods. Additionally, also incorporated outcome doxorubicin on morphology.

Language: Английский

Citations

14
Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research DOI Creative Commons
Claire Beckers, Lazaros Vasilikos,

Lorena Moor

et al.

Clinical and Translational Radiation Oncology, Journal Year: 2025, Volume and Issue: 51, P. 100920 - 100920

Published: Jan. 15, 2025

Language: Английский

Citations

0
Tumor Spheroid Uptake of Fluorescent Nanodiamonds Is Limited by Mass Density: A 4D Light-Sheet Assay DOI Creative Commons
Μαρία Νιώρα, Martin Dufva, Liselotte Jauffred

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Language: Английский

Citations

0
HDAC6 inhibition through WT161 synergizes with temozolomide, induces apoptosis, reduces cell motility, and decreases β-catenin levels in glioblastoma cells DOI

Leilane Sales Oliveira,

João Marcos Oliveira‐Silva, Hebréia O. Almeida-Souza

et al.

Investigational New Drugs, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Language: Английский

Citations

0
Pentagalloyl Glucose Targets the JAK1/JAK3-STAT3 Pathway to Inhibit Cancer Stem Cells and Epithelial–Mesenchymal Transition in 5-Fluorouracil-Resistant Colorectal Cancer DOI Creative Commons
Chengli Wen,

Xu Zhang,

Jiraporn Kantapan

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156773 - 156773

Published: April 1, 2025

Language: Английский

Citations

0
Novel half Salphen cobalt(iii) complexes: synthesis, DNA binding and anticancer studies DOI
Riccardo Bonsignore,

Elisa Trippodo,

Roberto Di Gesù

et al.

Dalton Transactions, Journal Year: 2024, Volume and Issue: 53(14), P. 6311 - 6322

Published: Jan. 1, 2024

Novel DNA binding half Salphen cobalt( iii )complexes with promising anticancer activity on 3D cultured models.

Language: Английский

Citations

3
Encapsulation of Human Spinal Cord Progenitor Cells in Hyaluronan-Gelatin Hydrogel for Spinal Cord Injury Treatment DOI
Christy Kwokdinata,

Vaibavi Ramanujam,

Jiahui Chen

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(44), P. 50679 - 50692

Published: Sept. 26, 2023

Transplanting human induced pluripotent stem cells (iPSCs)-derived spinal cord progenitor (SCPCs) is a promising approach to treat injuries. However, cell therapies face challenges in survival, localization the targeted site, and control of differentiation. Here, we encapsulated SCPCs thiol-modified hyaluronan-gelatin hydrogels optimized scaffold mechanical properties encapsulation density promote viability neuronal differentiation vitro vivo. Different compositions formulated by varying concentrations poly(ethylene glycol) diacrylate were mechanically characterized using atomic force microscopy. In SCPC study showed higher proliferation with lower substrate Young's modulus (200 Pa vs 580 Pa) density. Moreover, soft facilitated degree extended filament structures contrast clumped cellular morphologies obtained stiff (p < 0.01). When transplanted vivo, SCPC-encapsulated resulted survival at region as compared delivery without hydrogel 2 weeks postimplantation within rat Notably, immunostaining demonstrated that hydrogel-encapsulated differentiated along oligodendroglial lineages The lack pluripotency also supported safety transplantation approach. Overall, injectable shows promise supporting neural after into cord.

Language: Английский

Citations

8
Research Methods and New Advances in Drug–Drug Interactions Mediated by Renal Transporters DOI Creative Commons
Kexin Lin, Xiaorui Kong, Xufeng Tao

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(13), P. 5252 - 5252

Published: July 6, 2023

The kidney is critical in the human body’s excretion of drugs and their metabolites. Renal transporters participate actively secreting substances from proximal tubular cells reabsorbing them distal renal tubules. They can affect clearance rates (CLr) metabolites, eventually influence clinical efficiency side effects drugs, may produce drug–drug interactions (DDIs) significance. transporter-mediated DDIs have also been studied by many researchers. In this article, main types vitro research models used for study are membrane-based assays, cell-based slice uptake model. vivo include animal experiments, gene knockout models, positron emission tomography (PET) technology, studies on beings. addition, vitro–in extrapolation (IVIVE), ex perfusion (EVKP) and, more recently, biomarker methods silico included. This article reviews traditional DDIs, updates recent progress development methods, then classifies summarizes advantages disadvantages each method. Through sorting work conducted paper, it will be convenient researchers at different learning stages to choose best method own based subject’s situation when they going mediated transporters.

Language: Английский

Citations

6