bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 27, 2023
Abstract
A
myriad
of
biological
effects
can
be
stimulated
by
the
ultrasound
(US)
for
treatment
cancer.
The
objective
our
research
was
to
investigate
effect
alone
and
in
combination
with
chemotherapeutic
drugs
such
as
doxorubicin
(DOX)
temozolomide
(TMZ)
on
human
glioblastoma
(GBM)
epidermoid
carcinoma
cancer
2D
3D
cell
cultures.
Results
indicated
that
US
96-probe
device
could
induce
tumour
sphere
cytotoxicity
a
dosage-
time-dependent
manner,
multiple
treatments
augmenting
this
cytotoxic
effect.
With
enhanced
cytotoxicity,
decreased
growth
metabolic
activity,
disrupted
spheroid
integrity,
heightened
occurrence
DNA
double
strand
breaks,
resulting
damage
spheres
an
inability
rebuild
after
treatments.
TMZ
/
DOX
efficiency
GBM
enhancing
induced
compared
monolayer
cells,
also
increasing
incubation
time,
which
is
most
crucial
way
differentiate
between
effectiveness
drug
without
US.
In
conclusion,
data
demonstrate
enhances
diffusion,
uptake,
using
models
when
It
demonstrates
significance
culture
delivery
discovery
research.
Graphical
Cancers,
Journal Year:
2023,
Volume and Issue:
15(21), P. 5162 - 5162
Published: Oct. 26, 2023
Cancer
stem
cells
(CSCs)
are
considered
to
be
responsible
for
recurrence,
metastasis,
and
resistance
treatment
in
many
types
of
cancers;
therefore,
new
strategies
targeting
CSCs
attracting
attention.
In
this
study,
we
fabricated
a
polyethylene
glycol-tagged
microwell
device
that
enabled
spheroid
formation
from
human
oral
squamous
carcinoma
cells.
HSC-3
Ca9-22
cultured
the
aggregated
generated
single
per
well
within
24–48
h.
The
circular
shape
smooth
surface
spheroids
were
maintained
up
five
days,
most
comprising
Calcein
AM-positive
viable
Interestingly,
mRNA
expression
CSC
markers
(Cd44,
Oct4,
Nanog,
Sox2)
significantly
higher
than
monolayer
cultures.
marker-positive
observed
throughout
spheroids.
Moreover,
cisplatin
was
enhanced
spheroid-cultured
compared
monolayer-cultured
Furthermore,
some
marker
genes
upregulated
outgrown
xenograft
model,
tumor
growth
implantation
group
comparable
culture
group.
These
results
suggest
our
system
may
high-throughput
tool
producing
uniform
large
numbers
cancer
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 27, 2023
Abstract
A
myriad
of
biological
effects
can
be
stimulated
by
the
ultrasound
(US)
for
treatment
cancer.
The
objective
our
research
was
to
investigate
effect
alone
and
in
combination
with
chemotherapeutic
drugs
such
as
doxorubicin
(DOX)
temozolomide
(TMZ)
on
human
glioblastoma
(GBM)
epidermoid
carcinoma
cancer
2D
3D
cell
cultures.
Results
indicated
that
US
96-probe
device
could
induce
tumour
sphere
cytotoxicity
a
dosage-
time-dependent
manner,
multiple
treatments
augmenting
this
cytotoxic
effect.
With
enhanced
cytotoxicity,
decreased
growth
metabolic
activity,
disrupted
spheroid
integrity,
heightened
occurrence
DNA
double
strand
breaks,
resulting
damage
spheres
an
inability
rebuild
after
treatments.
TMZ
/
DOX
efficiency
GBM
enhancing
induced
compared
monolayer
cells,
also
increasing
incubation
time,
which
is
most
crucial
way
differentiate
between
effectiveness
drug
without
US.
In
conclusion,
data
demonstrate
enhances
diffusion,
uptake,
using
models
when
It
demonstrates
significance
culture
delivery
discovery
research.
Graphical
