DNA metabarcoding reveals unexpected diet breadth of the specialist large‐headed resin bee (Heriades truncorum) in urbanised areas across Germany DOI Creative Commons
Makaylee K. Crone, Felix Fornoff, Alexandra‐Maria Klein

et al.

Insect Conservation and Diversity, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Abstract Host plants required by specialist bee species may be limited in urban areas. We investigated the effects of urbanisation on Heriades truncorum , a solitary that is plant family Asteraceae. examined brood cell number, host and native pollen proportions (via DNA metabarcoding) nutritional composition provisions H. nests. Increasing resulted fewer cells higher genus diversity provisions. Bees collected from non‐native non‐Asteraceae genera, including new records for . overall was associated with decreased numbers. However, an increased Asteraceae genera number. The proportion were not changes Pollen provision related to or averaged P:L ratio ~1:1, similar These results suggest are equally beneficial Germany. Moreover, at more sites suggests level resources, particularly plants, could highly landscapes. While high conservation concern, our data demonstrate mechanisms which narrow less common specificity experience stress areas provides mitigation strategies.

Language: Английский

Towards holistic insect monitoring: species discovery, description, identification and traits for all insects DOI Creative Commons
Rudolf Meier, Emily Hartop, Christian Pylatiuk

et al.

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1904)

Published: May 5, 2024

Holistic insect monitoring needs scalable techniques to overcome taxon biases, determine species abundances, and gather functional traits for all species. This requires that we address taxonomic impediments the paucity of data on abundance, biomass traits. We here outline how these deficiencies could be addressed at scale. The workflow starts with large-scale barcoding (megabarcoding) specimens from mass samples obtained biomonitoring sites. barcodes are then used group into molecular operational units subsequently tested/validated as a second source (e.g. morphology). New described using barcodes, images short diagnoses, abundance collected both new specimen discovery become raw material training artificial intelligence identification algorithms collecting trait such body size, feeding modes. Additional can vouchers by genomic tools developed ecologists. Applying this pipeline few per site will lead greatly improved regardless whether composition sample is determined images, metabarcoding or megabarcoding. article part theme issue 'Towards toolkit global biodiversity monitoring'.

Language: Английский

Citations

15
Abundance estimation with DNA metabarcoding – recent advancements for terrestrial arthropods DOI Creative Commons
Wiebke Sickel, Vera Zizka, Alice Scherges

et al.

Metabarcoding and Metagenomics, Journal Year: 2023, Volume and Issue: 7

Published: Nov. 23, 2023

Biodiversity is declining at alarming rates worldwide and large-scale monitoring urgently needed to understand changes their drivers. While classical taxonomic identification of species time labour intensive, the combination with DNA-based methods could upscale activities achieve larger spatial coverage increased sampling effort. However, challenges remain for when number individuals per and/or biomass estimates are required. Several methodological advancements exist improve potential DNA metabarcoding abundance analysis, which however need further evaluation. Here, we discuss laboratory, as well some bioinformatic adjustments workflows regarding estimation from arthropod community samples. Our review includes pre-laboratory processing such specimen photography, laboratory use spike-in an internal standard like correction factors. We conclude that photography coupled currently promises greatest estimates, but approaches spike-ins factors promising pursue further.

Language: Английский

Citations

11
La reconnaissance des espèces basée sur l’ADN : applications, perspectives et défisen milieu continental terrestre DOI Open Access

H. Le Borgne,

Christophe Bouget

Naturae, Journal Year: 2024, Volume and Issue: 2024(3)

Published: Feb. 12, 2024

Face aux changements globaux actuels, l’enjeu des suivis de la dynamique biodiversité est croissant et entraîne une forte demande d’évaluations rapides détaillées biodiversité. L’identification moléculaire espèces plus en utilisée pour remplacer ou compléter les méthodes surveillance écologique classiques. Le metabarcoding considéré comme un outil d’inventaire, connaissance biologie (prédateurs proies, pollinisateurs, etc.) même découverte l’histoire d’écosystèmes. Il permet générer données sur manière rapide, précise fiable, large éventail d’organismes. Ce type méthodologie particulièrement intéressant observatoires dépourvus l’expertise nécessaire distinguer nombreuses groupes hyper diversifiés insectes ceux difficiles à inventorier. La reconnaissance partir l’ADN d’échantillons environnementaux (ADNe), tels que l’eau, sédiments, le sol, l’air diverses matières organiques, a champ d’application. Par son caractère non invasif destructif, ces approches sont importantes l’évaluation déontologique Les chercheurs intègrent l’ADNe dans leurs études biosurveillance raison sa précision facilité déploiement. Dans ce document, nous donnons aperçu champs d’application basées suivi biodiversité, d’acquisition données, traitement classification espèces, évoquons défis inhérents chacune étapes.

Citations

1
Introduction to the special issue: Pollen diversity, vegetation history and range shift in the (sub)tropics through the Cenozoic DOI
Limi Mao, Kangyou Huang, Huasheng Huang

et al.

Review of Palaeobotany and Palynology, Journal Year: 2024, Volume and Issue: unknown, P. 105277 - 105277

Published: Dec. 1, 2024

Language: Английский

Citations

1
DNA metabarcoding reveals unexpected diet breadth of the specialist large‐headed resin bee (Heriades truncorum) in urbanised areas across Germany DOI Creative Commons
Makaylee K. Crone, Felix Fornoff, Alexandra‐Maria Klein

et al.

Insect Conservation and Diversity, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Abstract Host plants required by specialist bee species may be limited in urban areas. We investigated the effects of urbanisation on Heriades truncorum , a solitary that is plant family Asteraceae. examined brood cell number, host and native pollen proportions (via DNA metabarcoding) nutritional composition provisions H. nests. Increasing resulted fewer cells higher genus diversity provisions. Bees collected from non‐native non‐Asteraceae genera, including new records for . overall was associated with decreased numbers. However, an increased Asteraceae genera number. The proportion were not changes Pollen provision related to or averaged P:L ratio ~1:1, similar These results suggest are equally beneficial Germany. Moreover, at more sites suggests level resources, particularly plants, could highly landscapes. While high conservation concern, our data demonstrate mechanisms which narrow less common specificity experience stress areas provides mitigation strategies.

Language: Английский

Citations

0