The combination of Ephedrae herba and coixol from Coicis semen attenuate adiposity via glucocorticoid receptor regulation DOI Creative Commons
Ga‐Ram Yu, Jai‐Eun Kim, Dong‐Woo Lim

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 8, 2023

Abstract The enhanced therapeutic effects and mechanisms of certain herbal combination in various prescriptions are mostly unclear. A two herbs, namely Ephedrae herba (EH) Coicis semen (CS), has been commonly prescribed for obesity. In our previous work, the EH CS was studied using network pharmacological approach to predict its targets vitro experiments evaluate efficacy on Although we demonstrated anti-adiposity matured adipocytes, molecular contributing compounds underlying EH-CS adiposity or adipogenesis were not fully elucidated. current study adopted integrated bioinformatics analysis precisely validate potential by screening differentially expressed genes (DEGs) morbid obesity patients from NCBI Gene Expression Omnibus (GEO). Based functional cluster down-regulated DEGs, anti-adipogenesis mechanism speculated with KEGG enrichment analysis. Furthermore, investigated combinational coixol, stigmasterol, which expected have main beneficial metabolic diseases. Moreover, distinct effect transcriptional activity glucocorticoid receptor (GR) electrophoretic mobility shift assay (EMSA). predicted modulate involved pathways crucial disease morbidly obese individuals. significantly increased phosphorylation acetyl-coA carboxylase (ACC), AMP-activated protein kinase (AMPK), B (AKT) 3T3-L1 cells decreased intracellular lipid accumulation. anti-adipogenesis/lipogenesis inhibiting gene expression levels. Finally, coixol inhibited dexamethasone-induced GR translocation nucleus binding adipocytes. could be considered a strategy treating diseases, including

Language: Английский

Planta Medica DOI
Mounia Chroho, Christian Bailly, Latifa Bouissane

et al.

Planta Medica, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Treatise on Febrile Diseases" refers to the classic traditional Chinese medicine prescription Ge Gen decoction, which consists of seven herbs, including an Ephedra species [7], and a Latin textbook from roman naturalist Pliny Elder (Gaius Plinius Secundus, AD 23-79) underlined use as follows: "it is given black austere wine, crushed, for coughs, sighs, convulsions, drinking" [8].For decades, have provided herbal remedies treat human diseases [9,10].These medicinal

Language: Английский

Citations

11
The combination of Ephedrae herba and coixol from Coicis semen attenuate adiposity via glucocorticoid receptor regulation DOI Creative Commons
Ga‐Ram Yu, Jai‐Eun Kim, Dong‐Woo Lim

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 21, 2023

The enhanced therapeutic effects and mechanisms of certain herbal combination in various prescriptions are mostly unclear. A two herbs, namely Ephedrae herba (EH) Coicis semen (CS), has been commonly prescribed for obesity. In our previous work, the EH CS was studied using network pharmacological approach to predict its targets vitro experiments evaluate efficacy on Although we demonstrated anti-adiposity matured adipocytes, molecular contributing compounds underlying EH-CS adiposity or adipogenesis were not fully elucidated. current study adopted integrated bioinformatics analysis precisely validate potential by screening differentially expressed genes (DEGs) morbid obesity patients from NCBI gene expression omnibus (GEO). Based functional cluster down-regulated DEGs, anti-adipogenesis mechanism speculated with KEGG enrichment analysis. Furthermore, investigated combinational coixol, stigmasterol, which expected have main beneficial metabolic diseases. Moreover, distinct effect transcriptional activity glucocorticoid receptor (GR) electrophoretic mobility shift assay (EMSA). predicted modulate involved pathways crucial disease morbidly obese individuals. significantly increased phosphorylation acetyl-coA carboxylase (ACC), AMP-activated protein kinase (AMPK), B (AKT) 3T3-L1 cells decreased intracellular lipid accumulation. anti-adipogenesis/lipogenesis inhibiting levels. Finally, coixol inhibited dexamethasone-induced GR translocation nucleus binding adipocytes. could be considered a strategy treating diseases, including

Language: Английский

Citations

3
Ethnobotanical Uses and Pharmacological Activities of Moroccan Ephedra Species DOI Creative Commons
Mounia Chroho, Christian Bailly, Latifa Bouissane

et al.

Planta Medica, Journal Year: 2024, Volume and Issue: 90(05), P. 336 - 352

Published: Feb. 29, 2024

Abstract Ephedra species are among the most popular herbs used in traditional medicine for a long time. The ancient Chinese medical book “Treatise on Febrile Diseases” refers to classic prescription Ge Gen decoction, which consists of seven herbs, including an species. utilized all over world treat symptoms common cold and coughs, combat major human diseases, such as asthma, cancers, diabetes, cardiovascular digestive disorders, microbial infections. This study aimed at identifying specific traditionally Morocco therapeutic purposes. plant parts, their preparation process, treated pathologies were identified analyzed. results revealed five ethnobotanically important Ephedra: alata Decne, altissima Desf., distachya L., fragilis nebrodensis Tineo. These treating people with cancer, rheumatism, hypertension, influenza virus infection, respiratory ailments. In addition, they occasionally calefacient agents, regulate weight, or capillary care. Few studies have underlined antibacterial antioxidant activities some these Moroccan species, but little information is available regarding natural products origin bioactivities. Further phytochemical investigations clinical data encouraged better support use plants.

Language: Английский

Citations

0
None DOI Creative Commons
Mounia Chroho, Christian Bailly, Latifa Bouissane

et al.

Planta Medica, Journal Year: 2024, Volume and Issue: 90(05)

Published: April 16, 2024

Treatise on Febrile Diseases" refers to the classic traditional Chinese medicine prescription Ge Gen decoction, which consists of seven herbs, including an Ephedra species [7], and a Latin textbook from roman naturalist Pliny Elder (Gaius Plinius Secundus, AD 23-79) underlined use as follows: "it is given black austere wine, crushed, for coughs, sighs, convulsions, drinking" [8].For decades, have provided herbal remedies treat human diseases [9,10].These medicinal

Language: Английский

Citations

0
Graph convolutional network learning model based on new integrated data of the protein-protein interaction network and network pharmacology for the prediction of herbal medicines effective for the treatment of metabolic diseases DOI Creative Commons
Tae-Hyoung Kim, Ga‐Ram Yu, Dong‐Woo Lim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Abstract Chronic metabolic diseases constitute a group of conditions requiring long-term management and hold significant importance for national public health medical care. Currently, in Korean medicine, there are no insurance-covered herbal prescriptions designated primarily the treatment diseases. Therefore, objective this study was to identify from existing pool options that could be effective treating This research employed graph convolutional network learning model analyze PPI constructed pharmacology, aiming suitable various diseases, thus diverging literature-based approaches based on classical indications. Additionally, derived medicine candidates were subjected transfer binarily classified marketed drugs into those currently used not data-based verification. GCN, adept at capturing patterns within protein-protein interaction (PPI) networks, utilized classifying data. Moreover, gene scores related extracted GeneCards as weights. The performance pre-trained validated through 5-fold cross-validation bootstrapping with 100 iterations. Furthermore, ascertain superior our proposed model, number layers varied, each evaluated. Our structure achieved outstanding drugs, an average precision 96.68%, recall 97.18%, F1 score 96.74%. trained predicted most decoction would Jowiseunggi-tang hyperlipidemia, Saengmaegsan hypertension, Kalkunhaeki-tang type 2 diabetes. is first its kind integrate GCN weighted data classify by their potential usage certain

Language: Английский

Citations

0
A Study on the Effects and Mechanisms of the Combination Extract of Ephedrae Herba and Coicis Semen on Lipid Accumulation and Glucose Absorption in Non-Alcoholic Fatty Liver Disease DOI Open Access
Ga‐Ram Yu,

Hye-Lin Jin,

Dong‐Woo Lim

et al.

Journal of Korean Medicine for Obesity Research, Journal Year: 2024, Volume and Issue: 24(1), P. 1 - 12

Published: June 30, 2024

Objectives: Ephedrae herba (EH) and Coicis semen (CS) has been frequently prescribed for the treatment of obesity.However, effects combinational extracts these two herbs on non-alcoholic fatty liver disease are unknown.The aim present study was to investigate EH CS lipid accumulation glucose absorption in free acids (FFAs) or palmitic acid (PA)-treated HepG2 cells.Methods: Five samples were extracted by combination ratios (S1=0:100, S2=25:75, S3=50:50, S4=75:25, S5=100:0).Oil Red O staining used measure FFAs-induced steatosis cells.Intracellular triglycerides total cholesterol levels measured steatotic cells.In PA-treated cells, intracellular 2-NBDG detected using a fluorescence microplate reader flow cytometry.Phosphorylation key metabolism-related factors AMP-activated protein kinase acetyl-CoA carboxylase, expression synthesis-related carnitine palmitoyltransferase 1 alpha (CPT1α), sterol regulatory element-binding (SREBP1), peroxisome proliferator-activated receptor gamma (PPARγ) CCAAT enhancer binding (C/EBPα) confirmed western blot.Results: Treatment EH-CS cells significantly reduced accumulation.As relative ratio increased, lipid-lowering increased.However, S1 S5 did not reduce induced FFAs.However, restored PA-induced cells.Major makers SREBP1, PPARγ, C/EBPα, CPT1α tended decrease with ratio.Conclusions: The advantages over sole improving metabolism models.

Language: Английский

Citations

0
The combination of Ephedrae herba and coixol from Coicis semen attenuate adiposity via glucocorticoid receptor regulation DOI Creative Commons
Ga‐Ram Yu, Jai‐Eun Kim, Dong‐Woo Lim

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 8, 2023

Abstract The enhanced therapeutic effects and mechanisms of certain herbal combination in various prescriptions are mostly unclear. A two herbs, namely Ephedrae herba (EH) Coicis semen (CS), has been commonly prescribed for obesity. In our previous work, the EH CS was studied using network pharmacological approach to predict its targets vitro experiments evaluate efficacy on Although we demonstrated anti-adiposity matured adipocytes, molecular contributing compounds underlying EH-CS adiposity or adipogenesis were not fully elucidated. current study adopted integrated bioinformatics analysis precisely validate potential by screening differentially expressed genes (DEGs) morbid obesity patients from NCBI Gene Expression Omnibus (GEO). Based functional cluster down-regulated DEGs, anti-adipogenesis mechanism speculated with KEGG enrichment analysis. Furthermore, investigated combinational coixol, stigmasterol, which expected have main beneficial metabolic diseases. Moreover, distinct effect transcriptional activity glucocorticoid receptor (GR) electrophoretic mobility shift assay (EMSA). predicted modulate involved pathways crucial disease morbidly obese individuals. significantly increased phosphorylation acetyl-coA carboxylase (ACC), AMP-activated protein kinase (AMPK), B (AKT) 3T3-L1 cells decreased intracellular lipid accumulation. anti-adipogenesis/lipogenesis inhibiting gene expression levels. Finally, coixol inhibited dexamethasone-induced GR translocation nucleus binding adipocytes. could be considered a strategy treating diseases, including

Language: Английский

Citations

0