Frontiers in Microbiomes, Journal Year: 2024, Volume and Issue: 3

Published: Oct. 4, 2024

Introduction Pathogen introduction and transmission at the farm, regional, or national level are associated with reduced animal welfare negative impacts on herd economics. Ongoing infectious disease surveillance, active passive, is therefore of high importance. For optimal resolution, each pig sampled individually, for example by collecting blood nasal swabs. In recent years, oral fluids have become very useful population surveillance pen level. Another alternative sampling air to capture pathogens circulating across entire barn via bioaerosols. Objective This study aimed examine potential utility bioaerosol metagenomics pathogen detection farms. Methods Bioaerosols automated sampler, fluid pen-based ropes, were collected from two Scottish indoor All samples subjected conventional routine bacterial isolation. Total genomic nucleic acids extracted PCR screening three DNA viruses, Mycoplasma species an RNA virus. Illumina shotgun metagenomic sequencing was also conducted. Results Oral contained more compared samples. integrity exhibited limited impact sequence yield. While Streptococcus suis could be cultured a single sample, reads mapped S. detectable in all Other pathogens, including hyorhinis , M. hyopneumoniae hyosynoviae detected aerosols metagenomics. One farms PRRSV positive, virus but not Antimicrobial resistance (AMR) gene profiles had less variation between bioaerosols fluids. Some identified AMR genes strikingly similar abundance overall. Conclusion Overall, these findings indicate that there farms; however, research needed technical cost optimization allow livestock

Language: Английский