Long-Read–Based Hybrid Genome Assembly and Annotation of Snow Algal Strain CCCryo 101-99 (cf. Sphaerocystis sp., Chlamydomonadales)
Genome Biology and Evolution,
Journal Year:
2024,
Volume and Issue:
16(7)
Published: June 27, 2024
Abstract
Polar
regions
harbor
a
diversity
of
cold-adapted
(cryophilic)
algae,
which
can
be
categorized
into
psychrophilic
(obligate
cryophilic)
and
cryotrophic
(nonobligate
snow
algae.
Both
accumulate
significant
biomasses
on
glacier
habitats
play
major
roles
in
global
climate
dynamics.
Despite
their
significance,
genomic
studies
these
organisms
remain
scarce,
hindering
our
understanding
evolutionary
history
adaptive
mechanisms
the
face
change.
Here,
we
present
draft
genome
assembly
annotation
algal
strain
CCCryo
101-99
(cf.
Sphaerocystis
sp.).
The
haploid
is
122.5
Mb
length
represented
by
664
contigs
with
an
N50
0.86
Mb,
Benchmarking
Universal
Single-Copy
Orthologs
(BUSCO)
completeness
92.9%
(n
=
1,519),
maximum
contig
5.3
guanine-cystosine
(GC)
content
53.1%.
In
total,
28.98%
(35.5
Mb)
contains
repetitive
elements.
We
identified
417
noncoding
RNAs
annotated
chloroplast
genome.
predicted
proteome
comprises
14,805
genes
BUSCO
97.8%.
Our
preliminary
analyses
reveal
higher
repeat
compared
mesophilic
chlorophyte
relatives,
alongside
enrichment
gene
families
associated
photosynthesis
flagella
functions.
current
data
will
facilitate
future
comparative
studies,
improving
likely
response
polar
algae
to
warming
as
well
trajectories
permanently
cold
environments.
Language: Английский
Telomerase-independent survival leads to a mosaic of complex subtelomere rearrangements inChlamydomonas reinhardtii
Genome Research,
Journal Year:
2023,
Volume and Issue:
33(9), P. 1582 - 1598
Published: Aug. 14, 2023
Telomeres
and
subtelomeres,
the
genomic
regions
located
at
chromosome
extremities,
are
essential
for
genome
stability
in
eukaryotes.
In
absence
of
canonical
maintenance
mechanism
provided
by
telomerase,
telomere
shortening
induces
instability.
The
landscape
ensuing
rearrangements
is
not
accessible
short-read
sequencing.
Here,
we
leverage
Oxford
Nanopore
Technologies
long-read
sequencing
to
survey
extensive
repertoire
telomerase
mutants
model
green
microalga
Language: Английский