Uncovering mercury accumulation and the potential for bacterial bioremediation in response to contamination in the Singalila National Park

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 29, 2025

Several recent investigations into montane regions have reported on excess mercury accumulation in high-altitude forest ecosystems. This study explored the Singalila National Park, located ridge of Eastern Himalayas, revealing substantial contamination. Particular focus was Sandakphu (3636 m), highest peak West Bengal, India. It harboured 6.77 ± 0.01 mg/kg total its topsoil. Further evidence provided by leaves (0.040 mg/kg), and roots (0.150 0.008 mg/kg) local vegetation, litterfall (0.234 0.019 mosses (0.367 0.043 surface water from lakes waterbodies (0.010 0.005 mg/l), fresh snow (0.014 0.004 sleet (0.019 0.009 mg/l). Samples other points varying elevation park also demonstrated The soil displayed a range 0.068-5.28 mg/kg, while mean concentration 0.153 0.105 0.106 0.054 leaf litter 0.240 0.112 mg/kg. Additionally, microbial consortia isolated contaminated high tolerance to mercuric chloride, presumably gained through repeated consistent exposure. Four bacterial strains, MTS2C, MTS3A, MTS4B MTS6A, were further characterized for potential use bioremediation strategies. Their removal capacities determined be 82.35%, 75.21%, 61.95%, 37.47%, respectively. Overall, findings presented provide highly environment that poses significant ecological risk flora, fauna inhabitants this biodiversity hotspot. research highlights need exploration monitoring Himalayas role as sink atmospheric mercury.

Language: Английский

---

In Vivo Study on the Salivary Kinetics of Two Probiotic Strains Delivered via Chewing Gum

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 721 - 721

Published: March 24, 2025

Probiotics are increasingly used to promote oral health, with Lacticaseibacillus rhamnosus demonstrating proven effectiveness. Additionally, Heyndrickxia coagulans shows promising potential in this field. Chewing gum has recently been proposed as an innovative delivery method for probiotics. This study aimed evaluate the kinetics saliva of SNZ1969® and GG microencapsulated non-microencapsulated forms (LGG®) following their administration via sugar-free chewing gums. A randomized cross-over trial was conducted involving 10 volunteers. Participants chewed gums containing one probiotic strains min. Saliva samples were collected at baseline (T0) six subsequent time points over 2 h (T1–T6). Colony-forming units (CFUs) identified quantified. The Tukey’s range test applied make pairwise comparisons between different probiotics every point, same probiotic, area under curve describing saliva. At T1, all exhibited peak counts, followed by a gradual decline until T6. H. achieved highest counts T2, T3 (mean log10 CFU/mL: 6.1 ± 0.5; 5.8 5.6 0.5, respectively), while form LGG® peaked T4, T5, T6 4.0 0.7; 3.8 0.9; 3.3 1.3, respectively). participants reported no adverse effects. detectable up post-administration gum, indicating its suitability vehicle. However, significant variability observed among participants.

Language: Английский

---

Comparative study of photodynamic therapy with LED and probiotics in the treatment of halitosis: protocol for a randomised controlled clinical trial

BMJ Open, Journal Year: 2025, Volume and Issue: 15(4), P. e095544 - e095544

Published: April 1, 2025

Introduction Halitosis is a term that defines any unpleasant odour smell originating from the oral cavity and may have local or systemic origin. This project aims to determine effectiveness of treatment involving antimicrobial photodynamic therapy (aPDT) combined with using probiotics at reducing halitosis. Methods analysis 92 individuals 18 60 years age diagnosis halitosis (sulfide≥112 ppb, gas chromatography) will be selected. The participants randomly allocated four groups (n=23). Group 1 (control): brushing, dental floss tongue scraper; group 2: floss, scraper aPDT blue Light Emitting Diode (LED) +annatto; 3: +annatto probiotic lozenges containing Streptococcus salivarius K12 (BLIS K12); 4: S. K12). Comparisons made respiratory results before immediately after first session, end 30-day period again days initiation. Microbiological (counts colony-forming units viable bacteria coated tongue) performed same time. microbiome conducted treatment, 30 completion initiation, following DNA extraction. All receive hygiene instructions as well brushes, toothpaste floss. Data normality checked Shapiro-Wilk test. In case normality, variance used for comparisons. non-parametric data, Kruskal-Wallis test used. Wilcoxon analyse each between two assessment times. Ethics dissemination protocol has been approved by Human Research Committee Nove de Julho University (certificate number: 82830524.6.0000.5511; approval date: 2 October 2024). Participants agree take part in study signing an informed consent form. findings published peer-reviewed journal. collected data available OSF repository. Trial registration number NCT06583720 .

Language: Английский

---

Modern aspects of individual tongue hygiene (literature review)

Medical alphabet, Journal Year: 2025, Volume and Issue: 10, P. 20 - 24

Published: May 21, 2025

There are many methods of cleaning the tongue, ranging from simple ones such as rubbing with fingers or a spoon, to specialized tools scrapers and brushes. Studies show that few patients practice daily tongue hygiene. Surveys reveal low awareness language among certain groups population. Tongue play an important role in oral hygiene by removing biofilm food residues, which helps improve health fight bad breath. Hygiene contributes prevention dental diseases. Scrapers made various materials metal, plastic wood, each has different properties. Metal durable easy sterilize, options more affordable. Wooden quite environmentally friendly. The shapes also vary: U-shaped similar shape while T-shaped provide good access surface tongue. Regular cleansing reduces breath improves Although some studies do not identify difference between using toothbrushes scrapers, others suggest effective at reducing volatile sulfur compounds (VSC). Recently, researchers have studied other types ultrasonic microcurrent generating scrapers. However, improper use may cause irritation injury. special for hygiene, when used correctly, they help remove plaque, prevent breath, taste perception.

Language: Английский

---

Efeito do raspador lingual de polietilenotereftalato na remoção da saburra lingual

Research Society and Development, Journal Year: 2024, Volume and Issue: 13(8), P. e14213846683 - e14213846683

Published: Sept. 1, 2024

O objetivo deste trabalho foi comparar um raspador de língua obtido garrafas polietilenotereftalato (PET) com modelo comercial (COM). Um desenho paralelo, controlado e randomizado usado. Dos 61 voluntários do Departamento Odontologia da Universidade Federal Sergipe, 30 atenderam aos critérios inclusão exclusão para distribuição aleatória em 2 grupos: PET (n=15) COM (n=15). Na consulta inicial, o Índice Saburra Lingual Winkel (ISL-W) medido antes (T0) imediatamente após (T1) remoção saburra lingual realizada pelo profissional, que forneceu instruções uso caseiro vezes ao dia. Após 14 dias, ISL-W mensurado T0 T1, feita participante. 30, 1 grupo não retornou dias. Os dados obtidos T1 foram submetidos teste t-Student pareado (α=0,05). As diferenças entre transformadas valores percentuais analisadas t Student das consultas inicial dias resultados indicaram os raspadores reduziram significantemente (P<0,05), redução semelhantes: (34±36%) (32±22%); houve significante dos (P<0,01). Concluiu-se capaz remover a lingual, semelhantes COM, reduzindo caseiro.

---