Single-cell and bulk RNA sequencing-based screening and identification of extracellular trap network-related genes in neutrophils in acute myocardial infarction DOI Creative Commons
Wei Li, Jun Yang

Medicine, Journal Year: 2024, Volume and Issue: 103(47), P. e40590 - e40590

Published: Nov. 22, 2024

Background: The neutrophil-mediated generation of neutrophil extracellular traps (NETs) results in an augmented inflammatory response and cellular tissue injury during acute myocardial infarction (AMI). Through the analysis public database information, we discovered confirmed putative critical genes involved NETs-mediated AMI. Methods: AMI dataset GSE66360 single-cell GSE163465 were downloaded from Gene Expression Omnibus database. Key screened by bioinformatics. Quantitative real-time PCR (qRT-PCR) was used to verify key genes, then a Mendelian randomization (MR) study conducted on basis genome-wide association determine causal relationship between Dimensionality reduction clustering, pseudo-time series, cell communication performed analyze bulk RNA sequencing dynamic evolution NETs process. Immunohistochemistry Western blot Results: Six IL1β, S100A12, TLR2, CXCL1, CXCL2, CCL4, out through qRT-PCR showed that compared with control group, expression 5 upregulated group. In MR study, CXCL1 CCL4 observed have Single-cell NETs-related IL1β highly expressed. Combining single cells, MR, gene selected as focus study. H9c2 cardiomyocytes simulated under hypoxia, increased. immunohistochemical Conclusions: We found 6 related damage causally may play important role Our provide some insights into

Language: Английский

Clinical significance of microRNA-328-3p and bone metabolism biomarkers in gout patients with different musculoskeletal ultrasonography imaging DOI Creative Commons
Qingqing Song,

Lifang Xue,

Jie Ren

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 1, 2025

MicroRNA (miRNA) participates in the pathophysiological processes of multiple metabolic diseases, including gout. In gout patients, there is concomitant derangement bone metabolism. The study aimed to explore correlation different ultrasonic manifestations and miR-328-3p levels with markers patients. A total 320 patients were grouped according musculoskeletal ultrasound (MSUS) imaging. Serum osteocalcin (BGP), C-terminal telopeptide type I collagen (CTX-I) osteopotin (OPN) detected evaluate via qRT-PCR. Pearson's analysis was performed relationships between variables. Patients tophi accompanied by erosion demonstrated significantly elevated BGP OPN compared those or aggregate MSU crystals absence erosions. Cases long course disease exhibited more severe destruction. without specific clinical presented highest serum miR-328-3p, whereas erosions lowest values. Significantly negative correlations also for values all associated diverse MSUS imaging are capable reflecting joint impairment

Language: Английский

Citations

0
[Protective Effect and Mechanism of miR-328-3p on Coronary Artery Endothelial Cell Injury Induced by Oxidized Low-density Lipoprotein]. DOI

Yonglan Hou,

Xia Li, Jianmei Wang

et al.

PubMed, Journal Year: 2024, Volume and Issue: 55(5), P. 1210 - 1216

Published: Sept. 20, 2024

To investigate the protective effect of miR-328-3p on oxidized low-density lipoprotein (ox-LDL)-induced coronary artery endothelial cell injury and potentially relevant mechanisms.

Language: Английский

Citations

0
Single-cell and bulk RNA sequencing-based screening and identification of extracellular trap network-related genes in neutrophils in acute myocardial infarction DOI Creative Commons
Wei Li, Jun Yang

Medicine, Journal Year: 2024, Volume and Issue: 103(47), P. e40590 - e40590

Published: Nov. 22, 2024

Background: The neutrophil-mediated generation of neutrophil extracellular traps (NETs) results in an augmented inflammatory response and cellular tissue injury during acute myocardial infarction (AMI). Through the analysis public database information, we discovered confirmed putative critical genes involved NETs-mediated AMI. Methods: AMI dataset GSE66360 single-cell GSE163465 were downloaded from Gene Expression Omnibus database. Key screened by bioinformatics. Quantitative real-time PCR (qRT-PCR) was used to verify key genes, then a Mendelian randomization (MR) study conducted on basis genome-wide association determine causal relationship between Dimensionality reduction clustering, pseudo-time series, cell communication performed analyze bulk RNA sequencing dynamic evolution NETs process. Immunohistochemistry Western blot Results: Six IL1β, S100A12, TLR2, CXCL1, CXCL2, CCL4, out through qRT-PCR showed that compared with control group, expression 5 upregulated group. In MR study, CXCL1 CCL4 observed have Single-cell NETs-related IL1β highly expressed. Combining single cells, MR, gene selected as focus study. H9c2 cardiomyocytes simulated under hypoxia, increased. immunohistochemical Conclusions: We found 6 related damage causally may play important role Our provide some insights into

Language: Английский

Citations

0