Development of viral infectious clones and their applications based on yeast and bacterial artificial chromosome platforms DOI Creative Commons
Yiyi Wu,

Shangqing Gao,

G. Liu

et al.

Molecular Biomedicine, Journal Year: 2025, Volume and Issue: 6(1)

Published: April 29, 2025

Abstract Infectious Clones represent a foundational technique in the field of reverse genetics, allowing for construction and manipulation full-length viral genomes. The main methods currently used constructing infectious clones include Transformation-associated recombination (TAR), which is based on Yeast Artificial Chromosome (YAC) Bacterial (BAC). YAC BAC systems are powerful tools that enable large DNA fragments, making them well-suited These have been successfully applied to construct wide range viruses, including coronaviruses, herpesviruses, flaviviruses baculoviruses. rescued recombinant viruses from these widely various research areas, such as vaccine development, antiviral drug screening, pathogenesis virulence studies, gene therapy vector design. However, different possess unique biological characteristics, challenge remains how rapidly obtain future research. In summary, this review introduced development applications clones, with focus YAC, combined YAC-BAC technologies. We emphasize importance platforms areas aim provide deeper insights can advance platform broaden its application horizons.

Language: Английский

Development of viral infectious clones and their applications based on yeast and bacterial artificial chromosome platforms DOI Creative Commons
Yiyi Wu,

Shangqing Gao,

G. Liu

et al.

Molecular Biomedicine, Journal Year: 2025, Volume and Issue: 6(1)

Published: April 29, 2025

Abstract Infectious Clones represent a foundational technique in the field of reverse genetics, allowing for construction and manipulation full-length viral genomes. The main methods currently used constructing infectious clones include Transformation-associated recombination (TAR), which is based on Yeast Artificial Chromosome (YAC) Bacterial (BAC). YAC BAC systems are powerful tools that enable large DNA fragments, making them well-suited These have been successfully applied to construct wide range viruses, including coronaviruses, herpesviruses, flaviviruses baculoviruses. rescued recombinant viruses from these widely various research areas, such as vaccine development, antiviral drug screening, pathogenesis virulence studies, gene therapy vector design. However, different possess unique biological characteristics, challenge remains how rapidly obtain future research. In summary, this review introduced development applications clones, with focus YAC, combined YAC-BAC technologies. We emphasize importance platforms areas aim provide deeper insights can advance platform broaden its application horizons.

Language: Английский

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