In Silico Design of miniACE2 Decoys with In Vitro Enhanced Neutralization Activity against SARS-CoV-2, Encompassing Omicron Subvariants DOI Open Access

Jenny Andrea Arévalo-Romero,

Gina López-Cantillo,

Sara Moreno-Jiménez

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10802 - 10802

Published: Oct. 8, 2024

The COVID-19 pandemic has overwhelmed healthcare systems and triggered global economic downturns. While vaccines have reduced the lethality rate of SARS-CoV-2 to 0.9% as October 2024, continuous evolution variants remains a significant public health challenge. Next-generation medical therapies offer hope in addressing this threat, especially for immunocompromised individuals who experience prolonged infections severe illnesses, contributing viral evolution. These cases increase risk new emerging. This study explores miniACE2 decoys novel strategy counteract variants. Using silico design molecular dynamics, blocking proteins (BPs) were developed with stronger binding affinity receptor-binding domain multiple than naturally soluble human ACE2. BPs expressed E. coli tested vitro, showing promising neutralizing effects. Notably, BP9 exhibited an average IC50 4.9 µg/mL across several variants, including Wuhan strain, Mu, Omicron BA.1, BA.2 low demonstrates potent ability BP9, indicating its efficacy at concentrations.Based on these findings, emerged therapeutic candidate combating evolving thereby positioning it potential emergency biopharmaceutical.

Language: Английский

The Contentious Origins of SARS-CoV-2: A Comprehensive Review of Current Knowledge DOI Creative Commons
José L. Domingo

Published: Feb. 11, 2025

The emergence of SARS-CoV-2 in late 2019 triggered a global pandemic (COVID-19) that has claimed millions lives and continues to impact public health systems worldwide. This review examines current scientific evidence regarding the virus's origins, focusing on two primary hypotheses: zoonotic spillover laboratory-related emergence. A comprehensive analysis peer-reviewed literature published between December 2023 January 2025 was conducted, identifying relevant studies through searches PubMed Scopus databases. revealed strong phylogenetic supporting natural origin, with several indicating close genetic relationships bat coronaviruses. Environmental sampling data from Huanan Seafood Market detected RNA high number environmental samples animal stalls. While preponderance supports spillover, absence definitively identified intermediate host maintains some uncertainty. concludes evidence-based recommendations for enhanced viral surveillance, implementation One Health approaches, strengthened international collaboration frameworks prevent future coronavirus pandemics.

Language: Английский

Citations

0
The Contentious Origins of SARS-CoV-2: A Comprehensive Review of Current Knowledge DOI Creative Commons
José L. Domingo

Published: Feb. 17, 2025

The emergence of SARS-CoV-2 in late 2019 triggered a global pandemic (COVID-19) that has claimed millions lives and continues to impact public health systems worldwide. This review examines current scientific evidence regarding the virus's origins, focusing on two primary hypotheses: zoonotic spillover laboratory-related emergence. A comprehensive analysis peer-reviewed literature primarily published between December 2023 January 2025 was conducted, identifying relevant studies through searches PubMed Scopus databases. Key earlier publications are also included provide essential background context. revealed strong phylogenetic supporting natural origin, with several indicating close genetic relationships bat coronaviruses. Environmental sampling data from Huanan Seafood Market detected RNA high number environmental samples animal stalls. While preponderance supports spillover, absence definitively identified intermediate host maintains some uncertainty. concludes evidence-based recommendations for enhanced viral surveillance, implementation One Health approaches, strengthened international collaboration frameworks prevent future coronavirus pandemics.

Language: Английский

Citations

0
The Contentious Origins of SARS-CoV-2: A Comprehensive Review of Current Knowledge DOI Creative Commons
José L. Domingo

Published: Feb. 19, 2025

The emergence of SARS-CoV-2 in late 2019 triggered a global pandemic (COVID-19) that has claimed millions lives and continues to impact public health systems worldwide. This review examines current scientific evidence regarding the virus's origins, focusing on two primary hypotheses: zoonotic spillover laboratory-related emergence. A comprehensive analysis peer-reviewed literature primarily published between December 2023 January 2025 was conducted, identifying relevant studies through searches PubMed Scopus databases. Key earlier publications are also included provide essential background context. revealed strong phylogenetic supporting natural origin, with several indicating close genetic relationships bat coronaviruses. Environmental sampling data from Huanan Seafood Market detected RNA high number environmental samples animal stalls. While preponderance supports spillover, absence definitively identified intermediate host maintains some uncertainty. concludes evidence-based recommendations for enhanced viral surveillance, implementation One Health approaches, strengthened international collaboration frameworks prevent future coronavirus pandemics.

Language: Английский

Citations

0
COVID-19: Lessons from the Past to Inform the Future of Healthcare DOI Creative Commons
Camilla Mattiuzzi, Giuseppe Lippi

COVID, Journal Year: 2024, Volume and Issue: 5(1), P. 4 - 4

Published: Dec. 26, 2024

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its global spread have left an indelible mark, disrupting multiple aspects human life. It is therefore crucial to retrospectively analyze the factors that contributed more initial inefficiency response, thus enhancing preparedness proactively addressing risk similar events occurring in future. Critical areas were identified based on our expertise. Relevant bibliographic references subsequently gathered through open search scientific databases substantiate concepts discussed this article. key issues hindered effective response disease 2019 (COVID-19) are numerous multifaceted, some these will be critically examined article, including delayed identification pathogen, inadequate public health preparedness, therapeutic management, deficiencies laboratory diagnostics. From analysis, for improvement emerge ensure efficient responses future crises, (i) strengthening information systems, (ii) improving pandemic planning, (iii) developing a resilient healthcare workforce, (iv) increasing investment research development, (v) expanding use telemedicine digital health, (vi) ensuring universal access healthcare, (vii) communication trust.

Language: Английский

Citations

2
In Silico Design of miniACE2 Decoys with In Vitro Enhanced Neutralization Activity against SARS-CoV-2, Encompassing Omicron Subvariants DOI Open Access

Jenny Andrea Arévalo-Romero,

Gina López-Cantillo,

Sara Moreno-Jiménez

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10802 - 10802

Published: Oct. 8, 2024

The COVID-19 pandemic has overwhelmed healthcare systems and triggered global economic downturns. While vaccines have reduced the lethality rate of SARS-CoV-2 to 0.9% as October 2024, continuous evolution variants remains a significant public health challenge. Next-generation medical therapies offer hope in addressing this threat, especially for immunocompromised individuals who experience prolonged infections severe illnesses, contributing viral evolution. These cases increase risk new emerging. This study explores miniACE2 decoys novel strategy counteract variants. Using silico design molecular dynamics, blocking proteins (BPs) were developed with stronger binding affinity receptor-binding domain multiple than naturally soluble human ACE2. BPs expressed E. coli tested vitro, showing promising neutralizing effects. Notably, BP9 exhibited an average IC50 4.9 µg/mL across several variants, including Wuhan strain, Mu, Omicron BA.1, BA.2 low demonstrates potent ability BP9, indicating its efficacy at concentrations.Based on these findings, emerged therapeutic candidate combating evolving thereby positioning it potential emergency biopharmaceutical.

Language: Английский

Citations

1