Immunogenicity of a New Recombinant Vaccine against Enterotoxemia and Infectious Necrotic Hepatitis in Ruminants DOI

Dilek Nur Ekinoğlu,

Venhar Çelik, Esra Gül

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Abstract Background Clostridial diseases are common in ruminants all over the world. The present study aimed to investigate efficacy of a new recombinant DNA vaccine developed against enterotoxemia and infectious necrotic hepatitis sheep. Results Recombinant vaccines (RV) prepared three different formulas (RV100, RV200 RV400), each containing 100, 200 400 µg unpurified proteins Clostridium perfringes type C beta toxin gene (CpCcpb), D epsilon (CpDetx) novyi B alpha (CnBtcn-alpha), were subjected potency testing rabbits. RV400 was preferred immunise sheep as antitoxin titres pooled blood serum rabbits administered with this determined be above minimum values specified European Pharmacopoeia. A total 24 Akkaraman breed no antibodies relevant toxins used for experiment. All animals groups (recombinant group, commercial polyvalent group negative control group), consisting eight sheep, vaccinated twice an interval 21 days and, measured 14 after second vaccination by mouse neutralization test. average immunised calculated 9.75 ± 1.28 IU/mL C. perfringens beta, 13.75 1.98 5.50 0.93 toxins. On other hand, detected 8.00 2.14 IU/mL, 4.25 1.67 IU/m L 6.50 C. perfringens alpha, respectively. No titre (PBS). statistically significant difference observed between terms (p = 0.0002). Conclusions first combined from results suggested that it could successfully protection these diseases.

Language: Английский

Edible Coatings: A Novel Approach to Extending the Shelf Life of Fruits and Vegetables DOI Open Access

Harshit Rajial,

Suraj Varma

Journal of Advances in Biology & Biotechnology, Journal Year: 2024, Volume and Issue: 27(7), P. 25 - 37

Published: June 3, 2024

The rapid deterioration of fruits and vegetables during pre- post-harvest conditions leads to significant losses, affecting both quality shelf life. Edible coatings have emerged as an effective solution mitigate these issues, providing a protective layer that can be safely consumed part the food. Recently, there has been growing interest in using herbal extracts such lemongrass, oregano, Aloe vera, Tulsi, mint, neem, cinnamon, clove for edible due their beneficial properties. These herbal-based shown prevent water loss, control ripening process respiration rate, delay oxidative browning, reduce microbial growth various vegetables, including grapes, papaya, oranges, cucumbers, tomatoes, apples, mangoes. Research indicates extend storage time fresh produce 15-35 days at low temperatures, compared 8-10 uncoated produce. use is promising alternative chemical treatments, offering innovative approach commercial application. not only life but also improve food appearance safety environmentally friendly nature. They derived from animal or vegetable sources may consist proteins, lipids, polysaccharides, resins, combinations thereof. Acting barriers moisture gases, enhance safety, often incorporating antimicrobial compounds. advantages include reduced packaging waste, extended life, protection environmental effects while maintaining transfer oxygen, carbon dioxide, moisture, aroma, taste. Given high market demand nutritious perishable nature, present method address challenges, benefiting consumers environment. This review discusses components, benefits, application techniques coatings, focusing on potential

Language: Английский

Citations

6

Immunogenicity of a New Recombinant Vaccine against Enterotoxemia and Infectious Necrotic Hepatitis in Ruminants DOI

Dilek Nur Ekinoğlu,

Venhar Çelik, Esra Gül

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Abstract Background Clostridial diseases are common in ruminants all over the world. The present study aimed to investigate efficacy of a new recombinant DNA vaccine developed against enterotoxemia and infectious necrotic hepatitis sheep. Results Recombinant vaccines (RV) prepared three different formulas (RV100, RV200 RV400), each containing 100, 200 400 µg unpurified proteins Clostridium perfringes type C beta toxin gene (CpCcpb), D epsilon (CpDetx) novyi B alpha (CnBtcn-alpha), were subjected potency testing rabbits. RV400 was preferred immunise sheep as antitoxin titres pooled blood serum rabbits administered with this determined be above minimum values specified European Pharmacopoeia. A total 24 Akkaraman breed no antibodies relevant toxins used for experiment. All animals groups (recombinant group, commercial polyvalent group negative control group), consisting eight sheep, vaccinated twice an interval 21 days and, measured 14 after second vaccination by mouse neutralization test. average immunised calculated 9.75 ± 1.28 IU/mL C. perfringens beta, 13.75 1.98 5.50 0.93 toxins. On other hand, detected 8.00 2.14 IU/mL, 4.25 1.67 IU/m L 6.50 C. perfringens alpha, respectively. No titre (PBS). statistically significant difference observed between terms (p = 0.0002). Conclusions first combined from results suggested that it could successfully protection these diseases.

Language: Английский

Citations

0