Screened potential dsRNA targets in the digestive system of adult flea beetle Phyllotreta striolata: psJHBP‐like and psaaNAT‐like for enhancing RNAi pest control

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract BACKGROUND The flea beetle Phyllotreta striolata is a major pest of Cruciferae crops, exhibiting strong resistance to chemical pesticides. RNA interference (RNAi) has evolved into an innovative method for managing pests. This study aims evaluate the potential spray‐induced gene silencing (SIGS) as management strategy P. by targeting genes involved in its digestive system. RESULTS Transcriptomic analysis indicated notable enrichment related nutrient metabolism, substrate synthesis, and energy metabolism within tract . Based on these findings, two genes, psJHBP‐like psaaNAT‐like , were selected target candidates because their high expression gut. Feeding beetles double‐stranded (dsRNA) solutions led effective mortality, particularly at concentration 1000 ng/μL. RNAi also induced morphological changes gut tissue cells. However, dsRNA concentrations (above ng/μL) triggered slight avoidance behavior beetles. Leaf‐coating experiments further demonstrated reduction leaf damage fecal morphology following treatment, suggesting disruption normal processes. CONCLUSION results indicate that aimed expressed system, such holds considerable promise To improve effectiveness RNAi‐based strategies, it critical optimize dsRNA. © 2025 Society Chemical Industry.

Language: Английский

Identification and Characterization of Troponin T Associated with Development, Metabolism and Reproduction in Tribolium castaneum

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2786 - 2786

Published: March 19, 2025

As a tropomyosin-binding component, troponin T (TnT) is essential for the Ca2+ regulation of striated muscles’ contraction and locomotion activity, but its impacts on growth development insects have rarely been reported. In this study, TnT was identified functionally characterized in Tribolium castaneum by RNA interference (RNAi) transcriptome analysis. The T. contained 1152 bp open reading frame encoding 383 amino acids. It displayed highest expression late pupae highly expressed integument CNS. Both larval early pupal injection dsTnT led to 100% cumulative mortality before pupal–adult transition. Late RNAi caused 26.01 ± 4.29% mortality; survivors successfully became adults, 49.71 6.51% died 10 days with dried shriveled abdomen, poorly developed reproductive system no offspring. Additionally, sequencing results indicated that key ecdysteroid juvenile hormone biosynthesis genes (CYP314A1, aldehyde dehydrogenase family 3 member B1 farnesol dehydrogenase) were affected, as well several cuticle protein, nutrition metabolism immune-related genes, suggesting may play prominent roles development, reproduction affecting these pathways. This study could provide brand-new target gene strategy pest control.

Language: Английский