Enterococcus faecium MBBL3 Exhibits Promising Probiotic Potential and Antimicrobial Efficacy Against Bovine Mastitis-Associated Escherichia coli and Klebsiella pneumoniae

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Enterococcus faecium , a promising probiotic, combats pathogens, supports gut health, strengthens immunity, and provides natural approach to address the escalating global challenge of antimicrobial resistance. This study aimed investigate genome E. MBBL3, isolated from healthy cow milk, assess its probiotic potential activity against pathogens causing bovine mastitis. The strain was analyzed through whole sequencing, along with in-vitro in-silico assessments were conducted determine efficacy mastitis Klebsiella pneumoniae MBBL2 ( Kp MBBL2) Escherichia coli MBBL4 Ec MBBL4). assembly functional annotations uncovered many important traits in where comparison revealed high genetic similarity other strains. MBBL3 demonstrated ability ferment wide range carbohydrates possessed 76 carbohydrate-active enzyme-related genes, including five key CAZy families namely GH73, GH18, CBM50, CE4, AA10. It also importance genes for bile salt acid tolerance, stress resistance, surface adhesion. Additionally, contained metabolite regions involved biosynthesis compounds such as 2,4-DAPG, aborycin, enterocin NKR-5-3B, sodorifen, bacteriocin gene clusters sactipeptides Enterolysin_A UviB . Safety indicated low pathogenic potential, while assays antibiotic susceptibility suppressed growth MBBL4, respectively. Its compound exhibited strong molecular interactions virulence proteins these pathogens. Therefore, especially combined safety, position it valuable candidate therapeutic applications. Key points genosme showed species this genera. Genetic makeup survival adaptation different niches host’s gut. In-vitro results, several linked antimicrobials combat

Language: Английский

Genomic and In Vitro Analysis of Pediococcus pentosaceus MBBL4 Implicated Its Therapeutic Use Against Mastitis Pathogens and as a Potential Probiotic

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Language: Английский