Novel Immunoglobulin Domain Proteins Provide Insights into Evolution and Pathogenesis Mechanisms of SARS-Related Coronaviruses

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: March 7, 2020

ABSTRACT A novel coronavirus (SARS-CoV-2) is the causative agent of an emergent severe respiratory disease (COVID-19) in humans that threatening to result a global health crisis. By using genomic, sequence, structural and evolutionary analysis, we show Alpha- Beta-CoVs possess several families immunoglobulin (Ig) domain proteins, including ORF8 ORF7a from SARS-related coronaviruses two protein groups certain Alpha-CoVs. Among them, distinguished being rapidly evolving, possessing unique insert hypervariable position among SARS-CoV-2 genomes its predicted ligand-binding groove. We also uncover many Ig proteins metazoan viruses which are distinct sequence structure but share architecture comparable CoV proteins. Hence, propose deployment widely-used strategy by viruses, potential pathogenicity factor evolves counter immune response facilitate transmission between hosts.

Language: Английский

---

Infection with the Persistent Murine Norovirus Strain MNV-S99 Suppresses IFN-Beta Release and Activation of Stat1 In Vitro

PLoS ONE, Journal Year: 2016, Volume and Issue: 11(6), P. e0156898 - e0156898

Published: June 13, 2016

Norovirus infection is the main cause of epidemic non-bacterial gastroenteritis in humans. Although human norovirus (HuNoV) self-limiting, it can persist for extended periods time immune deficient patients. Due to lack robust cell culture and small animal systems, little known about HuNoV pathogenicity. However, murine (MNV) be propagated used as a model study infection. Several MNV are mice. In this study, we show that strain MNV-S99 persists wild type inbred (C57BL/6J) mice over period at least 5 weeks post Viral RNA was detectable jejunum, ileum, cecum, colon, with highest titers colon cecum. To characterize effect on innate response, Stat1 phosphorylation IFN-β production were analyzed compared non-persistent MNV-1.CW3. While MNV-1.CW3 showed comparable growth characteristics vitro, release strongly decreased after conclusion, our results unlike MNV-1.CW3, establishes persistent mice, possibly due interfering response.

Language: Английский

---

New insights into the immunomodulatory properties of poxvirus cytokine decoy receptors at the cell surface

F1000Research, Journal Year: 2018, Volume and Issue: 7, P. 719 - 719

Published: June 11, 2018

Poxviruses encode a set of secreted proteins that bind cytokines and chemokines as strategy to modulate host defense mechanisms. These viral mimic the activity cytokine decoy receptors but have unique properties may enhance their activity. Here, we describe ability poxvirus attach cell surface after secretion from infected cells, discuss advantages this property confer these immunomodulatory proteins.

Language: Английский

---

Interaction between unrelated viruses during in vivo co-infection to limit pathology and immunity

Virology, Journal Year: 2015, Volume and Issue: 484, P. 153 - 162

Published: June 20, 2015

Language: Английский

---

Innate Immune Gene Transcript Level Associated with the Infection of Macrophages with Ectromelia Virus in Two Different Mouse Strains

Viral Immunology, Journal Year: 2017, Volume and Issue: 30(5), P. 315 - 329

Published: April 28, 2017

Poxviruses have evolved numerous mechanisms to avoid the immune response of infected host, and many these not been fully described. Here, we studied transcriptional innate genes in BALB/c C57BL/6 peritoneal macrophages following infection with Moscow strain ectromelia virus (ECTV-Mos) aim delineating that contribute difference between susceptibility resistance lethal infection. We show a generalized downregulation four categories (toll-like receptor signaling, NOD-like RIG-I-like type I interferon signaling) antiviral receptors, downstream signaling pathways, responsive components. Two important observations were made. First, 14 differentially expressed fold change upregulation two above occurring mice, known be resistant ECTV-Mos infection, whereas same downregulated mice below. Second, cathepsin group was both strains but profound changes 17, 38, 62 for CtsL, CtsB, CtsS, respectively, mice. poxvirus profoundly downregulates mRNA protein expression three cathepsins this appears support replication. Based on data propose variations gene observed may resistance/susceptibility by ECTV-Mos.

Language: Английский

---