HP1a-mediated heterochromatin formation promotes antimicrobial responses against Pseudomonas aeruginosa infection

BMC Biology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Oct. 20, 2022

Abstract Background Pseudomonas aeruginosa is a Gram-negative bacterium that causes severe infectious disease in diverse host organisms, including humans. Effective therapeutic options for P. infection are limited due to increasing multidrug resistance and it therefore critical understand the regulation of innate immune responses guide development effective options. The epigenetic mechanisms by which hosts regulate their antimicrobial against remain unclear. Here, we used Drosophila melanogaster investigate role heterochromatin protein 1a (HP1a), key regulator, its mediation formation PA14, highly virulent strain. Results Animals with decreased levels showed less infection. In contrast, flies increased formation, either whole organism or specifically fat body—an organ important humoral response—showed greater infection, as demonstrated survival reduced bacterial load. Increased body promoted via upregulation deficiency (imd) pathway-mediated peptides (AMPs) before middle stage AMPs were required elicit HP1a-mediated Moreover, downregulated early stage, but upregulated Conclusions These data indicate promotes epigenetically upregulating imd pathway. Our study provides novel molecular, cellular, organismal insights into new strategies targeting have potential combat

Language: Английский

NADPH Oxidases in Arthropods

Springer eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 477 - 488

Published: Jan. 1, 2023

Language: Английский

Delayed host mortality and immune response upon infection with P. aeruginosa persister cells

Infection and Immunity, Journal Year: 2023, Volume and Issue: 91(10)

Published: Sept. 21, 2023

Chronic infections are a heavy burden on healthcare systems worldwide. Persister cells thought to be largely responsible for chronic infection due their tolerance antimicrobials and recalcitrance innate immunity factors. Pseudomonas aeruginosa is common clinically relevant pathogen that contains stereotypical persister cells. Despite importance in infection, there have been limited efforts study cell vivo. Drosophila melanogaster has well-described immune response similar of vertebrates good candidate the development an vivo model Similar what observed other bacterial strains, this work we found with P. resulted delayed mortality phenotype Caenorhabditis elegans, Arabidopsis thaliana, D. compared regular An in-depth characterization infected loads differed between cells' during early stages. Furthermore, hemocyte activation antimicrobial peptide expression were delayed/reduced over same time course, indicating initial suppression of, or inability elicit, fly response. Overall, our findings support use as which vivo, where subpopulation exhibits virulence attenuated

Language: Английский