The Potential Role of Ocular and Otolaryngological Mucus Proteins in Myalgic Encephalomyelitis/ Chronic Fatigue Syndrome DOI Creative Commons

Kaylin Huitsing,

Tara Tritsch, Francisco J. Carrera Arias

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 24, 2023

Abstract Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating illness associated with constellation of other symptoms. While the most common symptom unrelenting fatigue, many individuals also report suffering from rhinitis, dry eyes and sore throat. Mucin proteins are responsible for contributing to formation mucosal membranes throughout body. These pathways contribute body’s defense mechanisms involving pathogenic onset. When compromised by pathogens epithelium releases numerous cytokines enters prolonged state inflammation eradicate any particular infection. Based on genetic analysis, computational theory modeling we hypothesize that mucin protein dysfunction may ME/CFS symptoms due inability form adequate layers body, especially in ocular otolaryngological leading low grade chronic exacerbation

Language: Английский

Differential Responses of Pediatric and Adult Primary Epithelial Cells to Human Metapneumovirus and Respiratory Syncytial Virus Infection DOI Creative Commons

Pius I. Babawale,

Antonieta Guerrero-Plata

Viruses, Journal Year: 2025, Volume and Issue: 17(3), P. 380 - 380

Published: March 6, 2025

Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are pneumoviruses causing lower tract infections, primarily in infants children rather than healthy adults. bronchial epithelial cells serve as a viral replication target source of the innate immune response to these viruses. To better understand responses induced by RSV HMPV pediatric airway epithelium, we comparatively studied adult responses. We used normal human (NHBE) cultured an air–liquid interface culture system (ALI), which helps mimic architecture epithelium. Our results demonstrate differential patterns reduced interferons; inflammatory cytokines’ expression compared cells. However, expressed increased mucus stronger pro-inflammatory monocyte-derived dendritic These findings reveal age-dependent that may contribute more severe infections RSV.

Language: Английский

Citations

1
Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner DOI Open Access

Yoshihisa Kaizuka,

Rika Machida

Published: Jan. 17, 2025

Various types of glycoproteins have been suggested to inhibit viral infection cells via steric repulsion. However, it is difficult evaluate such physical actions genetically, simply because they are nonspecific and can be caused by any molecule. Therefore, we investigated a method compare this action among with diverse membrane protein profiles. We found that wide range individually had strong inhibitory effect on infection, while the other hand, total amount glycans was negatively correlated level in each cell. Thus, infection-inhibitory molecularly nonspecific, but additively enhanced according cell surface. In correlation, function as fundamental factor. Further investigating mechanism which factor control, conclude repulsion between proteins created branched forms kinetic energy barrier against packing virus into region interstitial space. As result, formation adhesive interface membrane, necessary for inhibited. This study attempted link cell’s properties intracellular biochemicals. A similar approach may applied quantify biological phenomena.

Language: Английский

Citations

0
Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner DOI Open Access

Yoshihisa Kaizuka,

Rika Machida

Published: Jan. 17, 2025

Various types of glycoproteins have been suggested to inhibit viral infection cells via steric repulsion. However, it is difficult evaluate such physical actions genetically, simply because they are nonspecific and can be caused by any molecule. Therefore, we investigated a method compare this action among with diverse membrane protein profiles. We found that wide range individually had strong inhibitory effect on infection, while the other hand, total amount glycans was negatively correlated level in each cell. Thus, infection-inhibitory molecularly nonspecific, but additively enhanced according cell surface. In correlation, function as fundamental factor. Further investigating mechanism which factor control, conclude repulsion between proteins created branched forms kinetic energy barrier against packing virus into region interstitial space. As result, formation adhesive interface membrane, necessary for inhibited. This study attempted link cell’s properties intracellular biochemicals. A similar approach may applied quantify biological phenomena.

Language: Английский

Citations

0
Characterization and application in recombinant N-GlcNAc-protein production of a novel endo-β-N-acetylglucosaminidase from Listeria booriae DOI

Weian Mao,

Yongheng Rong,

Hongmei Zhang

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108290 - 108290

Published: Feb. 1, 2025

Language: Английский

Citations

0
Variable DPP4 expression in multiciliated cells of the human nasal epithelium as a determinant for MERS-CoV tropism DOI Creative Commons
Tim I. Breugem,

Samra Riesebosch,

Jing Shu Zhang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(11)

Published: March 6, 2025

Transmissibility of respiratory viruses is a complex viral trait that intricately linked to tropism. Several highly transmissible viruses, including severe acute syndrome coronavirus 2 and Influenza specifically target multiciliated cells in the upper tract facilitate efficient human-to-human transmission. In contrast, zoonotic Middle East (MERS-CoV) generally transmits poorly between humans, which largely attributed absence its receptor dipeptidyl peptidase 4 (DPP4) tract. At same time, MERS-CoV epidemiology characterized by occasional superspreading events, suggesting some individuals can disseminate this virus effectively. Here, we utilized well-differentiated human pulmonary nasal airway organoid-derived cultures further delineate tropism MERS-CoV. We find replicated high titers both cultures. Using single-cell messenger-RNA sequencing, immunofluorescence, immunohistochemistry, show preferentially targeted cells, leading loss ciliary coverage. cellular was dependent on differentiation cultures, replication efficiency varied considerably donors. Similarly, variable focal expression DPP4 revealed nose tissues. This study indicates may vary due differences expression, providing an explanation for unpredictable transmission pattern

Language: Английский

Citations

0
Synergistic effects of Candida albicans and Porphyromonas gingivalis biofilms on epithelial barrier function in a 3D aspiration pneumonia model DOI Creative Commons
Ewelina Wronowska, Ibeth Guevara-Lora, Aleksandra Brankiewicz

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 7, 2025

Introduction Polymicrobial infections involving Candida albicans and Porphyromonas gingivalis represent a significant challenge in maintaining epithelial barrier integrity. This study explores their synergistic effects on cells using an air-liquid interface (ALI) model. Methods Mixed-species biofilms were developed analyzed for impact permeability tight junction proteins. The of biofilm supernatants IL-8 secretion oxidative stress markers also evaluated. role P. proteases was assessed gingipain-null mutant (ΔKΔRAB) compared to the wild-type strain (W83). Survival experiments conducted Galleria mellonella larvae examine pathogenicity dual-species biofilms. Results significantly increased disrupted proteins, as evidenced by reduced levels ZO-1 E-cadherin. These changes accompanied stress, characterized decreased HO-1 expression enhanced Bax/Bcl-xL ratios, indicating pro-apoptotic activity. Supernatants from demonstrated pronounced effect cells, modulating exacerbating damage. C. identified dominant driver pro-inflammatory responses, while contributed through immune modulation enzymatic activity, primarily via gingipains. ΔKΔRAB caused less disruption wild-type, highlighting critical gingipains pathogenesis. Discussion supported these findings, survival associated with potential high-dose antimicrobial therapies mitigate this effect. results emphasize cooperative mechanisms compromising barriers underline importance combination targeting both fungal bacterial components polymicrobial infections.

Language: Английский

Citations

0
Oral SARS‐CoV‐2 Infection and Risk for Long Covid DOI Creative Commons

Joel Schwartz,

Kristelle J. Capistrano,

Heba Hussein

et al.

Reviews in Medical Virology, Journal Year: 2025, Volume and Issue: 35(2)

Published: March 1, 2025

ABSTRACT SARS‐CoV‐2 is an oral pathogen that infects and replicates in mucosal salivary epithelial cells, contributing to post‐acute sequelae COVID‐19 (PASC) other non‐oral pathologies. While pre‐existing inflammatory diseases provides a conducive environment for the virus, acute infection persistence of can also results microbiome dysbiosis further worsens poor health. Indeed, PASC includes periodontal diseases, dysgeusia, xerostomia, pharyngitis, keratoses, pulpitis suggesting significant bacterial contributions tissue tropism. Dysbiotic microbiome‐induced inflammation promote viral entry via angiotensin‐converting enzyme receptor‐2 (ACE2), serine transmembrane TMPRSS2 possibly non‐canonical pathways. Additionally, metabolites derived from dysbiotic alter physiological biochemical pathways related metabolism lipids, carbohydrates, amino acids. This may pro‐inflammatory microenvironment, leading immune exhaustion, loss tolerance, susceptibility variety pathogens, causing later chronic inflammation. Microbial release mimics host metallopeptidases furin, ADAM17 (A disintegrin metalloproteinase 17), glycoprotein aid attachment T cell immunoglobulin‐like (TIMs), enhancing while simultaneously depressing resistance clearance. Membrane reorganization characterised by neuroproteins, such as neuropilins, functionally assists with extends pathogenesis cavity brain, gut, or tissues. Thus, health, disrupted microbiomes tropism, weaken antiviral resistance, heightens infection. dysfunction increases risk additional infections, exacerbating conditions like endodontic diseases. These persistent health issues contribute systemic inflammation, creating bidirectional effects between tissues, potentially Post‐Acute Sequelae (PASC).

Language: Английский

Citations

0
Lacticaseibacillus rhamnosus CP-1 mitigates LPS-induced acute lung injury in mice via TLR/NF-κB pathway and gut microbiota modulation DOI

J.-S. Lin,

Zhonghua Wang, Jiaojiao Han

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106429 - 106429

Published: March 1, 2025

Language: Английский

Citations

0
Engineering a NanoBiT biosensor for detecting angiotensin-converting enzyme-2 (hACE2) interaction with SARS-CoV-2 spike protein and screening the inhibitors to block hACE2 and spike interaction DOI
Cheng‐Han Lin,

Xinrui Yang,

M. C. Lin

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 263, P. 116630 - 116630

Published: Aug. 3, 2024

Language: Английский

Citations

3
Milk antibody response after 3rd COVID-19 vaccine and SARS-CoV-2 infection and implications for infant protection DOI Creative Commons
Yarden Golan,

Mikias Ilala,

Lin Li

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(10), P. 107767 - 107767

Published: Aug. 30, 2023

Little is known about the persistence of human milk anti-SARS-CoV-2 antibodies after 2nd and 3rd vaccine doses infection following dose. In this study, milk, saliva, blood samples were collected from 33 lactating individuals before vaccination infection. Antibody levels measured using ELISA symptoms assessed questionnaires. We found that vaccination, persisted for up to 8 months. addition, distinct patterns IgA IgG production higher RBD-blocking activity was observed compared 3-dose vaccination. Infected mothers reported more than vaccinated mothers. examined in infant saliva breastfeeding abundant IgG. Our results emphasize importance improving secretion improve protection infants.

Language: Английский

Citations

8