Eosinophils protect against SARS-CoV-2 following a vaccine breakthrough infection DOI Open Access
Kathryn M. Moore, Stephanie L. Foster, Meenakshi Kar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Waning immunity and the emergence of immune evasive SARS-CoV-2 variants jeopardize vaccine efficacy leading to breakthrough infections. We have previously shown that innate cells play a critical role in controlling SARS-CoV-2. To investigate response during infections, we modeled infections by challenging low-dose vaccinated mice with vaccine-mismatched Beta variant. found infected had 2-log reduction lung viral burden, but increased cell infiltration parenchyma, characterized monocytes, monocyte-derived macrophages, eosinophils. Single RNA-seq revealed RNA was highly associated eosinophils corresponded unique IFN-γ biased signature. Antibody-mediated depletion resulted virus replication dissemination lungs, demonstrating lungs are protective These results highlight for mediated protection against

Language: Английский

Outcome of SARS-CoV-2 reinfection depends on genetic background in female mice DOI Creative Commons
Gagandeep Singh, Juan García-Bernalt Diego, Prajakta Warang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 23, 2024

Antigenically distinct SARS-CoV-2 variants increase the reinfection risk for vaccinated and previously exposed population due to antibody neutralization escape. COVID-19 severity depends on many variables, including host immune responses, which differ depending genetic predisposition. To address this, we perform profiling of female mice with different backgrounds –transgenic K18-hACE2 wild-type 129S1– infected severe B.1.351, 30 days after exposure milder BA.1 or H1N1. Prior infection protects against B.1.351-induced morbidity in but aggravates disease 129S1. H1N1 only Enhanced B.1.351 re-infected 129S1 is characterized by an IL-10, IL-1β, IL-18 IFN-γ, while cytokine profile resembles naïve undergoing their first viral infection. pathology during cannot be attributed weaker adaptive responses BA.1. Infection causes long-term differential remodeling transcriptional changes bronchioalveolar CD11c+ compartment. cells show a strong antiviral defense expression whereas present more pro-inflammatory response upon restimulation. In conclusion, induces cross-reactive 129S1, outcome correlates alveolar space. Genetic disposition can impact virus Here, authors used approach antigenically Omicron Beta that differences correlate mouse models background reinfection.

Language: Английский

Citations

2

Ally, adversary, or arbitrator? The context-dependent role of eosinophils in vaccination for respiratory viruses and subsequent breakthrough infections DOI Creative Commons
Lauren A. Chang, Michael Schotsaert

Journal of Leukocyte Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 30, 2024

Abstract Eosinophils are a critical type of immune cell and central players in 2 immunity. Existing literature suggests that eosinophils also can play role host antiviral responses, typically 1 events, against multiple respiratory viruses, both directly through release mediators indirectly activation other effector types. One way to prime responses toward effective is vaccination, where 1–skewed immunity desirable the context intracellular pathogens like viruses. In realm breakthrough viral infection vaccinated hosts, an event which virus still establish productive despite preexisting immunity, most prominently known for their link vaccine-associated enhanced disease upon natural syncytial infection. This was observed pediatric cohort during 1960s following vaccination with formalin-inactivated virus. More recent research has unveiled additional roles eosinophil The specific contribution quality vaccine efficacy, hosts remains largely unexplored, especially regarding potential protection. On basis current findings, we will speculate suggested function consider many ways by may exert protective pathological effects infections. We discuss how balance efficacy eosinophil-related risks, as well use products biomarkers or adverse events.

Language: Английский

Citations

1

Eosinophils protect against SARS-CoV-2 following a vaccine breakthrough infection DOI Open Access
Kathryn M. Moore, Stephanie L. Foster, Meenakshi Kar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Waning immunity and the emergence of immune evasive SARS-CoV-2 variants jeopardize vaccine efficacy leading to breakthrough infections. We have previously shown that innate cells play a critical role in controlling SARS-CoV-2. To investigate response during infections, we modeled infections by challenging low-dose vaccinated mice with vaccine-mismatched Beta variant. found infected had 2-log reduction lung viral burden, but increased cell infiltration parenchyma, characterized monocytes, monocyte-derived macrophages, eosinophils. Single RNA-seq revealed RNA was highly associated eosinophils corresponded unique IFN-γ biased signature. Antibody-mediated depletion resulted virus replication dissemination lungs, demonstrating lungs are protective These results highlight for mediated protection against

Language: Английский

Citations

0