Evaluation of interleukin-4 and some serological factors in children infected with cryptosporidiosis at Ramadi Teaching Hospital for women and children DOI Creative Commons

Mohannad Hamid Jasim,

Othman maen AlKubaisi,

Lima Tariq Youash Lazar

et al.

Journal of Applied and Natural Science, Journal Year: 2024, Volume and Issue: 16(4), P. 1485 - 1491

Published: Dec. 20, 2024

Cryptosporidiosis is a zoonotic disease caused by protozoan parasite of the genus Cryptosporidium. It widespread worldwide and among four main diarrhoea pathogens in children adults. The present study aimed to investigate infection with cryptosporidiosis under age eight years both sexes who suffered from diarrhoea, evaluate relationship Interlukin-4 parasitic changes some serological parameters that included lipid profile total protein. Two hundred fecal samples were collected Ramadi Teaching Hospital for Women Children. Microscopic examination stained Ziehl–Neelsen stain ELISA test indicated presence egg cysts 23 samples, percentage (11.5%). shape was spherical, tending oval, size (4.8 5.7 micrometres). results showed there significant increase cholesterol (Ch.), triglycerides (TG.), low density lipoprotein (LDL), very low-density (vLDL), high-density lipoproteins (HDL) patient's group, no difference between control group infected at (P ≤0.05). There level blood proteins (Total Protein, Albumin, Globulin) (P≤0.05). also differences interleukin-4 patients healthy people, which 0.0444 ± 0.01141 pg/ml Due increasing spread, seriousness, epidemiology parasite, considered environmental pollution, because its lack diagnosis health departments (such as hospitals), entry significantly stimulates immune system, causes dehydration death weak immunity, affects absorption fats, proteins, vitamins significantly, new factor IL-4 related identified. known how effect injury on amount fats are involved structure living cell membranes.

Language: Английский

Cryptosporidium Genomics — Current Understanding, Advances, and Applications DOI Creative Commons
Fiifi Agyabeng‐Dadzie, R. Xiao, Jessica C. Kissinger

et al.

Current Tropical Medicine Reports, Journal Year: 2024, Volume and Issue: 11(2), P. 92 - 103

Published: March 23, 2024

Here we highlight the significant contribution that genomics-based approaches have had on field of Cryptosporidium research and insights these generated into biology transmission. There are advances in genomics, genetic manipulation, gene expression, single-cell technologies. New better genome sequences revealed variable sub-telomeric families genes under selection. RNA expression data now include post-infection time points. These provided life cycle host-pathogen interactions. Antisense ncRNA transcripts abundant. The critical role dsRNA virus is becoming apparent. community's ability to identify genomic targets abundant, yet still lacking, collection data, combined with their increased assess function via knock-out, revolutionizing field. Advances detection virulence genes, surveillance, population recombination studies, epigenetics upon us.

Language: Английский

Citations

5
IL-27 limits HSPC differentiation during infection and protects from stem cell exhaustion DOI Creative Commons
Daniel L. Aldridge, Zachary Lanzar, Anthony T. Phan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Abstract Many inflammatory stimuli can induce progenitor cells in the bone marrow to produce increased numbers of myeloid as part process emergency myelopoiesis. These events are associated with innate training and have long-term impacts on hematopoietic stem cell (HSPC) development but also compromise their function. While many cytokines support myelopoiesis, less is known about mechanisms that temper these events. When mice lack cytokine IL-27 were infected Toxoplasma gondii , there was enhanced generation monocyte progenitors monocytes. In production localized HSPCs a survey receptor expression highlighted uniquely poised respond IL-27. Furthermore, use vitro differentiation assays mixed chimeras revealed from deficient pre-disposed towards lineage. Additional studies after infection loss IL-27R resulted reduced HSPC fitness manifested proliferative responses decreased ability reconstitute system. Thus, act provides regulatory brake limit induction preserve stemness.

Language: Английский

Citations

0
IL-27 limits HSPC differentiation during infection and protects from stem cell exhaustion DOI Open Access
Daniel L. Aldridge, Zachary Lanzar, Anthony T. Phan

et al.

Published: April 23, 2025

Many inflammatory stimuli can induce progenitor cells in the bone marrow to produce increased numbers of myeloid as part process emergency myelopoiesis. These events are associated with innate training and have long-term impacts on hematopoietic stem cell (HSPC) development but also compromise their function. While many cytokines support myelopoiesis, less is known about mechanisms that temper these events. When mice lack cytokine IL-27 were infected Toxoplasma gondii , there was enhanced generation monocyte progenitors monocytes. In production localized HSPCs a survey receptor expression highlighted uniquely poised respond IL-27. Furthermore, use vitro differentiation assays mixed chimeras revealed from deficient pre-disposed towards lineage. Additional studies after infection loss IL-27R resulted reduced HSPC fitness manifested proliferative responses decreased ability reconstitute system. Thus, act provides regulatory brake limit induction preserve stemness.

Language: Английский

Citations

0
IL-27 limits HSPC differentiation during infection and protects from stem cell exhaustion DOI Open Access
Daniel L. Aldridge, Zachary Lanzar, Anthony T. Phan

et al.

Published: April 23, 2025

Many inflammatory stimuli can induce progenitor cells in the bone marrow to produce increased numbers of myeloid as part process emergency myelopoiesis. These events are associated with innate training and have long-term impacts on hematopoietic stem cell (HSPC) development but also compromise their function. While many cytokines support myelopoiesis, less is known about mechanisms that temper these events. When mice lack cytokine IL-27 were infected Toxoplasma gondii , there was enhanced generation monocyte progenitors monocytes. In production localized HSPCs a survey receptor expression highlighted uniquely poised respond IL-27. Furthermore, use vitro differentiation assays mixed chimeras revealed from deficient pre-disposed towards lineage. Additional studies after infection loss IL-27R resulted reduced HSPC fitness manifested proliferative responses decreased ability reconstitute system. Thus, act provides regulatory brake limit induction preserve stemness.

Language: Английский

Citations

0
Parasite and host immune factors that impact the development of a mucosal vaccine for Cryptosporidium DOI Creative Commons
Maria Merolle, Boris Striepen, Christopher A. Hunter

et al.

Mucosal Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Intestinal cDC1s provide cues required for CD4+ T cell–mediated resistance to Cryptosporidium DOI Creative Commons
Ian S. Cohn, Bethan A. Wallbank, Breanne E. Haskins

et al.

The Journal of Experimental Medicine, Journal Year: 2024, Volume and Issue: 221(7)

Published: June 3, 2024

Cryptosporidium is an enteric pathogen and a prominent cause of diarrheal disease worldwide. Control requires CD4+ T cells, but how protective cell responses are generated poorly understood. Here, parasites that express MHCII-restricted model antigens were to understand the basis for priming effector function. These studies revealed parasite-specific cells primed in draining mesenteric lymph node differentiate into Th1 gut provide local parasite control. Although type 1 conventional dendritic (cDC1s) dispensable priming, they required homing source IL-12 at site infection promoted production IFN-γ. Thus, cDC1s have distinct roles shaping infection: first, promote from mesLN, second, drive intestine.

Language: Английский

Citations

2
Cryptosporidium impacts epithelial turnover and is resistant to induced death of the host cell DOI Creative Commons
Bethan A. Wallbank, Ryan D. Pardy, Igor E. Brodsky

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(8)

Published: July 12, 2024

Infection with the apicomplexan parasite

Language: Английский

Citations

2
Evaluation of interleukin-4 and some serological factors in children infected with cryptosporidiosis at Ramadi Teaching Hospital for women and children DOI Creative Commons

Mohannad Hamid Jasim,

Othman maen AlKubaisi,

Lima Tariq Youash Lazar

et al.

Journal of Applied and Natural Science, Journal Year: 2024, Volume and Issue: 16(4), P. 1485 - 1491

Published: Dec. 20, 2024

Cryptosporidiosis is a zoonotic disease caused by protozoan parasite of the genus Cryptosporidium. It widespread worldwide and among four main diarrhoea pathogens in children adults. The present study aimed to investigate infection with cryptosporidiosis under age eight years both sexes who suffered from diarrhoea, evaluate relationship Interlukin-4 parasitic changes some serological parameters that included lipid profile total protein. Two hundred fecal samples were collected Ramadi Teaching Hospital for Women Children. Microscopic examination stained Ziehl–Neelsen stain ELISA test indicated presence egg cysts 23 samples, percentage (11.5%). shape was spherical, tending oval, size (4.8 5.7 micrometres). results showed there significant increase cholesterol (Ch.), triglycerides (TG.), low density lipoprotein (LDL), very low-density (vLDL), high-density lipoproteins (HDL) patient's group, no difference between control group infected at (P ≤0.05). There level blood proteins (Total Protein, Albumin, Globulin) (P≤0.05). also differences interleukin-4 patients healthy people, which 0.0444 ± 0.01141 pg/ml Due increasing spread, seriousness, epidemiology parasite, considered environmental pollution, because its lack diagnosis health departments (such as hospitals), entry significantly stimulates immune system, causes dehydration death weak immunity, affects absorption fats, proteins, vitamins significantly, new factor IL-4 related identified. known how effect injury on amount fats are involved structure living cell membranes.

Language: Английский

Citations

0